Nucleic Acid Aptamers Increase the Anticancer Efficiency and Reduce the Toxicity of Cisplatin-Arabinogalactan Conjugates <i>In Vivo</i>

Zamay, Tatiana N.; Starkov, A. K.; Kolovskaya, Olga S.; Zamay, Galina S.; Veprintsev, Dmitry V.; Luzan, Natalia; Nikolaeva, Elena D.; Lukyanenko, Kirill A.; Artyushenko, P. V.; Shchugoreva, Irina A.; Glazyrin, Yury E.; Koshmanova, Anastasia A.; Krat, Alexey V.; Терешина, Д. С.; Zamay, Sergey S.; Pats, Yuriy S.; Zukov, Ruslan; Tomilin, Felix N.; Berezovski, Maxim V.; Kichkailo, Anna S.

doi:10.1089/nat.2022.0024

Cited by 4 publications

(4 citation statements)

References 38 publications

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“…A thiolated DNA aptamer (AS42) to EAC cells [27] capable of in vivo binding [28] and an oligonucleotide non-specific to EAC cells were used for the MND functionalization. The spatial structure of AS42 was described earlier [29]. MND functionalization (Figure 2) was performed according to the procedure described earlier in [26].…”

Section: Mnds and Their Functionalization With The Aptamersmentioning

confidence: 99%

Magnetic Nanoscalpel for the Effective Treatment of Ascites Tumors

Zamay

Luzan

et al. 2023

JFB

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One of the promising novel methods for radical tumor resection at a single-cell level is magneto-mechanical microsurgery (MMM) with magnetic nano- or microdisks modified with cancer-recognizing molecules. A low-frequency alternating magnetic field (AMF) remotely drives and controls the procedure. Here, we present characterization and application of magnetic nanodisks (MNDs) as a surgical instrument (“smart nanoscalpel”) at a single-cell level. MNDs with a quasi-dipole three-layer structure (Au/Ni/Au) and DNA aptamer AS42 (AS42-MNDs) on the surface converted magnetic moment into mechanical and destroyed tumor cells. The effectiveness of MMM was analyzed on Ehrlich ascites carcinoma (EAC) cells in vitro and in vivo using sine and square-shaped AMF with frequencies from 1 to 50 Hz with 0.1 to 1 duty-cycle parameters. MMM with the “Nanoscalpel” in a sine-shaped 20 Hz AMF, a rectangular-shaped 10 Hz AMF, and a 0.5 duty cycle was the most effective. A sine-shaped field caused apoptosis, whereas a rectangular-shaped field caused necrosis. Four sessions of MMM with AS42-MNDs significantly reduced the number of cells in the tumor. In contrast, ascites tumors continued to grow in groups of mice and mice treated with MNDs with nonspecific oligonucleotide NO-MND. Thus, applying a “smart nanoscalpel” is practical for the microsurgery of malignant neoplasms.

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Section: Mnds and Their Functionalization With The Aptamersmentioning

confidence: 99%

Magnetic Nanoscalpel for the Effective Treatment of Ascites Tumors

Zamay

Luzan

et al. 2023

JFB

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“…Cis-AG-Ap also successfully suppressed Ehrlich’s ascites carcinoma growth and the extent of tumor metastasis in vivo. The use of arabinogalactan and aptamers improved the bioavailability and efficiency of cisplatin, making this strategy very promising for targeted cancer therapy [ 62 ].…”

Section: Overview On Polysaccharide-based Drug Delivery Systemsmentioning

confidence: 99%

“…Cisplatin [62] Drug delivery systems with hepatocyte asialoglycoprotein receptor binding targeting the liver for in vivo antitumor efficacy. Self-assembled particles.…”

Section: Polysaccharide-based Drug Delivery Systems Classificationmentioning

confidence: 99%

Polysaccharide-Based Coatings as Drug Delivery Systems

Visan,

Cristescu

2023

Pharmaceutics

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Therapeutic polysaccharide-based coatings have recently emerged as versatile strategies to transform a conventional medical implant into a drug delivery system. However, the translation of these polysaccharide-based coatings into the clinic as drug delivery systems still requires a deeper understanding of their drug degradation/release profiles. This claim is supported by little or no data. In this review paper, a comprehensive description of the benefits and challenges generated by the polysaccharide-based coatings is provided. Moreover, the latest advances made towards the application of the most important representative coatings based on polysaccharide types for drug delivery are debated. Furthermore, suggestions/recommendations for future research to speed up the transition of polysaccharide-based drug delivery systems from the laboratory testing to clinical applications are given.

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“…Another example of ApDC was developed by conjugating aptamer AS-42 specific to heat-shock proteins Hspa8 and Hsp90ab1 with Cis-dichlorodiammineplatinum and Siberian larch arabinogalactan. This cisplatin-arabinogalactan-aptamer demonstrated a cytotoxicity effect against Ehrlich’s carcinoma [ 73 ]. Similarly, aptamer-siRNA chimeras injected intratumorally effectively targeted prostate-specific membrane antigens (PSMA) in vivo [ 74 ].…”

Section: Applications Of Aptamersmentioning

confidence: 99%

Aptamers Enhance Oncolytic Viruses’ Antitumor Efficacy

et al. 2022

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Oncolytic viruses are highly promising for cancer treatment because they target and lyse tumor cells. These genetically engineered vectors introduce therapeutic or immunostimulatory genes into the tumor. However, viral therapy is not always safe and effective. Several problems are related to oncolytic viruses’ targeted delivery to the tumor and immune system neutralization in the bloodstream. Cryoprotection and preventing viral particles from aggregating during storage are other critical issues. Aptamers, short RNA, or DNA oligonucleotides may help to crawl through this bottleneck. They are not immunogenic, are easily synthesized, can be chemically modified, and are not very demanding in storage conditions. It is possible to select an aptamer that specifically binds to any target cell, oncolytic virus, or molecule using the SELEX technology. This review comprehensively highlights the most important research and methodological approaches related to oncolytic viruses and nucleic acid aptamers. Here, we also analyze possible future research directions for combining these two methodologies to improve the effectiveness of cancer virotherapy.

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Nucleic Acid Aptamers Increase the Anticancer Efficiency and Reduce the Toxicity of Cisplatin-Arabinogalactan Conjugates In Vivo

Cited by 4 publications

References 38 publications

Magnetic Nanoscalpel for the Effective Treatment of Ascites Tumors

Magnetic Nanoscalpel for the Effective Treatment of Ascites Tumors

Polysaccharide-Based Coatings as Drug Delivery Systems

Aptamers Enhance Oncolytic Viruses’ Antitumor Efficacy

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