Nucleic acid-based electrochemical biosensors for rapid clinical diagnosis: advances, challenges, and opportunities

Hashem, Abu; Hossain, M. A. Motalib; Marlinda, Ab Rahman; Mamun, Mohammad Al; Sagadevan, Suresh; Shahnavaz, Zohreh; Simarani, Khanom; Johan, Mohd Rafie

doi:10.1080/10408363.2021.1997898

Cited by 33 publications

(19 citation statements)

References 166 publications

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“…Avoiding the complexity of the Western blot technique, we resorted to Dot blot analysis using PVDF membrane strips [10,20,21]. The PVDF membrane strips (0.45 µm) were activated by dipping into 100% Methanol for 15 sec, soaked in distilled water for 2 min, and directly equilibrated for 5 min by TBST buffer.…”

Section: Dot Blot Techniquementioning

confidence: 99%

“…The essences on which the different kits were built can be grouped into 1) Plate counting methods, 2) gold or similar nanoparticles methods, 3) solid phase flow cytometry methods, 4) PCR-based methods and 5) immunological methods [6][7][8]. All these kits rely on the use of expensive instrumentation which is complicated to operate, needs time, and were relatively expensive, [9][10][11]. Therefore the present study is designed to explore a simple, accurate, and easy tool for the detection of E.coli in food samples.…”

Section: Introductionmentioning

confidence: 99%

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Sensitivity and Reliability of Two Antibodies in Detecting E. coli in Meat and Water

Mashat¹,

Awad²,

Amin³

et al. 2022

Arch. Pharm. Pract

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Detection of E. coli in water and food constitutes a major challenge. However, the outer membrane proteins of this Gram-negative bacterium represent an excellent choice for their detection and diagnosis. Two outer membrane proteins: A and C, play important roles as drug modulators and in cellular permeability for this bacterium. Here we employed the antibodies raised against these two outer membrane proteins for the development of dot-blot immunoassays to detect this bacterium in water and meat samples. This immune-based assay was not only sensitive and reliable but rapid and cheap to perform. Sensitivities and specificities were demonstrated at the lowest detection limits of just two to three cells per milliliter, while the upper limit reached 107 cells per milliliter. In conclusion, the simplicity and extremely high resolution of this colored-based immunoassay test would be of great importance for untrained food and/or water inspectors or laboratory personnel to use with immediate visual results.

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Section: Dot Blot Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sensitivity and Reliability of Two Antibodies in Detecting E. coli in Meat and Water

Mashat¹,

Awad²,

Amin³

et al. 2022

Arch. Pharm. Pract

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“…Gold is utilised in a wide range of products, from high-end accessories to carefully guarded bars to tiny amounts in technological devices. Heterogeneous catalysis using gold nanoparticles has been a "hot issue" since it may be employed in a number of industrially and environmentally crucial oxidation reactions, such as sensor technologies and hydrogen fuel production [2][3][4][5][6][7][8], with new discoveries being made on a regular basis. In the last decade, the use of functionalized gold nanoparticles (AuNPs) for targeted sensing of particular biomolecules has become a popular research topic [4,9].…”

Section: Introductionmentioning

confidence: 99%

“…AuNP-based sensors are expected to change the principles of sensing and recognising biomolecules. In addition to sensing [3,10], gold nanoparticles are intriguing candidates for photothermal therapy, diagnostic, and drug delivery applications [10][11][12][13]. Due to their excellent properties such as highly resonant particle plasmons, conductivity, direct visualisation of single nanoclusters by light scattering, electrochemical properties, and colloidal AuNPs are appealing materials for many biotechnology applications.…”

Section: Introductionmentioning

confidence: 99%

Scrap Gold Recovery: Recycling, Fabrication and Electrochemical Characterization of Low-Cost Gold Electrode

Mamun

Wahab²,

Hossain³

et al. 2022

MCIJ

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As a noble metal, gold is considered the most popular material in electrocatalysis. In spite of that, this metal is a highly expensive material in the manufacture of low-cost appliances. In the present work, scrap gold, produced in jewellery as waste, is introduced as a low-cost resource of gold to be recycled. A gold electrode was fabricated by connecting the tablet shaped recycled gold with copper wire inserting into a polytetrafluoroethylene (PTFE) tube. The manufactured electrodes are easily renewable, modifiable with nanomaterials and show excellent electrochemical characteristics in presence of redox species. The CV (cyclic voltammetry), DPV (differential pulse voltammetry), and EIS (electrochemical impedance spectroscopic) analysis data also infer that the developed recycled gold-based electrodes would be suitable for the development of low-cost electrocatalytic sensors devices.

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“…Nanomaterials may significantly enhance biosensor performance, stability, repeatability, and sensitivity [35][36][37][38][39]. Among various nanomaterials, graphene (Gr) [40] and gold nanoparticles (AuNPs) [32] based nanocomposites are well established [41] due to their excellent performance.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

Hashem¹,

Hossain²,

Marlinda³

et al. 2022

Beilstein J. Nanotechnol.

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The Southeast Asian box turtle, Cuora amboinensis, is an ecologically important endangered species which needs an onsite monitoring device to protect it from extinction. An electrochemical DNA biosensor was developed to detect the C. amboinensis mitochondrial cytochrome b gene based on an in silico designed probe using bioinformatics tools, and it was also validated in wet-lab experiments. As a detection platform, a screen-printed carbon electrode (SPCE) enhanced with a nanocomposite containing gold nanoparticles and graphene was used. The morphology of the nanoparticles was analysed by field-emission scanning electron microscopy and structural characteristics were analysed by using energy-dispersive X-ray, UV–vis, and Fourier-transform infrared spectroscopy. The electrochemical characteristics of the modified electrodes were studied by cyclic voltammetry, differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy. The thiol-modified synthetic DNA probe was immobilised on modified SPCEs to facilitate hybridisation with the reverse complementary DNA. The turtle DNA was distinguished based on hybridisation-induced electrochemical change in the presence of methylene blue compared to their mismatches, noncomplementary, and nontarget species DNA measured by DPV. The developed biosensor exhibited a selective response towards reverse complementary DNAs and was able to discriminate turtles from other species. The modified electrode displayed good linearity for reverse complementary DNAs in the range of 1 × 10−11–5 × 10−6 M with a limit of detection of 0.85 × 10−12 M. This indicates that the proposed biosensor has the potential to be applied for the detection of real turtle species.

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Nucleic acid-based electrochemical biosensors for rapid clinical diagnosis: advances, challenges, and opportunities

Cited by 33 publications

References 166 publications

Sensitivity and Reliability of Two Antibodies in Detecting E. coli in Meat and Water

Sensitivity and Reliability of Two Antibodies in Detecting E. coli in Meat and Water

Scrap Gold Recovery: Recycling, Fabrication and Electrochemical Characterization of Low-Cost Gold Electrode

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

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