Nucleic Acid Based Testing (NABing): A Game Changer Technology for Public Health

Khera, Harvinder Kour; Mishra, Rakesh

doi:10.1007/s12033-023-00870-4

Mol Biotechnol

2023

DOI: 10.1007/s12033-023-00870-4

|View full text |Cite

Nucleic Acid Based Testing (NABing): A Game Changer Technology for Public Health

Harvinder Kour Khera,

Rakesh Mishra

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

2025

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 137 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Duplex in-one-tube detection of two important shrimp farming pathogens with color indication using a method combining RPA and PfAgo

Chen,

Yu,

Kang

et al. 2025

Aquaculture

View full text Add to dashboard Cite

Duplex in-one-tube detection of two important shrimp farming pathogens with color indication using a method combining RPA and PfAgo

Chen,

Yu,

Kang

et al. 2025

Aquaculture

View full text Add to dashboard Cite

A comprehensive review of dengue with a focus on emerging solutions for precision and timely detection

Stanley,

Khera,

Chandrasingh

et al. 2024

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Split Probe-Induced Protein Translational Amplification for Nucleic Acid Detection

Min,

Hong,

Kim

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Nucleic acid detection is important in a wide range of applications, including disease diagnosis, genetic testing, biotechnological research, environmental monitoring, and forensic science. However, the application of nucleic acid detection in various fields is hindered by the lack of sensitive, accurate, and inexpensive methods. This study introduces a simple approach to enhance the sensitivity for the accurate detection of nucleic acids. Our approach combined a split-probe strategy with in vitro translational amplification of reporter protein for signal generation to detect nucleic acids with high sensitivity and selectivity. This approach enables target-mediated translational amplification of reporter proteins by linking split probes in the presence of a target microRNA (miRNA). In particular, the fluorescence split-probe sensor adopts a reporter protein with various fluorescence wavelength regions, enabling the simultaneous detection of multiple target miRNAs. Moreover, luminescence detection by merely altering the reporter protein sequence can substantially enhance the sensitivity of detection of target miRNAs. Using this system, we analyzed and quantified target miRNAs in the total RNA extracted from cell lines and cell-derived extracellular vesicles with high specificity and accuracy. This split-probe sensor has potential as a powerful tool for the simple, sensitive, and specific detection of various target nucleic acids.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Nucleic Acid Based Testing (NABing): A Game Changer Technology for Public Health

Cited by 3 publications

References 137 publications

Duplex in-one-tube detection of two important shrimp farming pathogens with color indication using a method combining RPA and PfAgo

Duplex in-one-tube detection of two important shrimp farming pathogens with color indication using a method combining RPA and PfAgo

A comprehensive review of dengue with a focus on emerging solutions for precision and timely detection

Split Probe-Induced Protein Translational Amplification for Nucleic Acid Detection

Contact Info

Product

Resources

About