Nucleic acid probes as potential tools in oral microbial epidemiology

Albandar, Jasim M.; Olsen, Ingar

doi:10.1111/j.1600-0528.1990.tb00025.x

Cited by 4 publications

(1 citation statement)

References 58 publications

(70 reference statements)

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“…They are the periodontopathogens Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola, and caries agents-namely, Streptococcus mutans-group species. In fact, because of the high prevalence of periodontal diseases and caries, oral infections are a main indication for DNA probe application [1][2][3][4][5].…”

mentioning

confidence: 99%

DNA Probes and Primers in Dental Practice

Conrads

2002

CLIN INFECT DIS

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In clinical microbiology, molecular genetic techniques are increasingly being used to detect and/or differentiate uncultivable, anaerobic, or fastidious microorganisms. During the past decade, DNA probe hybridization and in vitro amplification by polymerase chain reaction have also been introduced to detect oral pathogens. The present review describes experiences with methods and commercial test systems for the detection of pathogens in periodontitis and caries.

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confidence: 99%

DNA Probes and Primers in Dental Practice

Conrads

2002

CLIN INFECT DIS

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Nucleic Acid Hybridization for Identification and Detection of Gram-Negative Anaerobes

Göbel¹,

Pelz²

1993

Brock/Springer Series in Contemporary Bioscience

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Associations between six DNA probe-detected periodontal bacteria and alveolar bone loss and other clinical signs of periodontitis

Albandar

Olsen

Gjermo

1990

Acta Odontologica Scandinavica

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The purpose of the present study was to assess the associations between the presence and amounts of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, B. intermedius, Eikenella corrodens, Wolinella recta, and Fusobacterium nucleatum in the periodontal pocket and the degree of alveolar bone loss and other clinical signs of periodonitis, such as probing pocket depth, attachment level, and presence of bleeding on probing at the same site. The study material comprised 16 subjects with or without approximal sites showing longitudinal alveolar bone loss who were selected from a group of 142 subjects monitored radiographically over the past 4 years. In this group 105 sites were examined, of which 58 showed recent alveolar bone loss greater than or equal to 1 mm. Subgingival plaque was collected with absorbent paper points and hybridized with 32P-labeled DNA probes specific for the above-mentioned bacteria. The amount of each bacterial species was correlated with the degree of bone loss over time and the three clinical measurements by means of Spearman rank correlation. A. actinomycetemcomitans showed poor correlations with all three clinical signs of periodontal inflammation, whereas B. gingivalis and W. recta demonstrated significant positive correlations with the three clinical measurements and with attachment level and pocket depth, respectively. In addition, the amount of A. actinomycetemcomitans, B. gingivalis and W. recta showed significant positive correlation with the extent of alveolar bone loss at the site. In contrast, the amounts of B. intermedius, E. corrodens, and F. nucleatum showed negative correlations with all four measurements.(ABSTRACT TRUNCATED AT 250 WORDS)

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Nucleic acid probes as potential tools in oral microbial epidemiology

Cited by 4 publications

References 58 publications

DNA Probes and Primers in Dental Practice

DNA Probes and Primers in Dental Practice

Nucleic Acid Hybridization for Identification and Detection of Gram-Negative Anaerobes

Associations between six DNA probe-detected periodontal bacteria and alveolar bone loss and other clinical signs of periodontitis

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