Nucleic acid sensing by regenerable surface-associated isothermal rolling circle amplification

McCarthy, Erik L.; Bickerstaff, Lee E.; Cunha, M. Pereira da; Millard, Paul J.

doi:10.1016/j.bios.2006.05.001

Cited by 12 publications

(4 citation statements)

References 39 publications

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“…When combined with microarray technology isothermal amplification can potentially open up new horizons as a low-cost, highly sensitive diagnostic tool for home use and/or personalized medicine. In particular, rolling circle amplification (RCA) [54], loop mediated isothermal amplification (LAMP) [55], helicase dependent amplification (HDA) [56] and strand displacement amplification [57][58][59]) are the most widely used techniques for isothermal DNA/RNA amplification. RCA is one of the simplest isothermal amplification processes in which one single-strand primer is extended by DNA polymerase with strand displacement activity using a circular DNA as a template.…”

Section: Isothermal Amplification On Microarraysmentioning

confidence: 99%

Recent developments in nucleic acid identification using solid-phase enzymatic assays

Khodakov

Ellis

2014

Microchim Acta

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Section: Isothermal Amplification On Microarraysmentioning

confidence: 99%

Recent developments in nucleic acid identification using solid-phase enzymatic assays

Khodakov

Ellis

2014

Microchim Acta

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“…Hence, various kinds of nucleic acid amplication methods have been developed to improve the sensitivity. [3][4][5][6][7][8][9] The uorescent labeling approach has been widely used in these methods for signal readout due to its sensitivity and easy labeling process. Nevertheless, when it comes to multiplex analysis, the uorescent labeling method oen suffers from photobleaching, spectral overlap and the lack of ability for highlevel multiplex target analysis.…”

Section: Introductionmentioning

confidence: 99%

ICP-MS DNA assay based on lanthanide labels and hybridization chain reaction amplification

et al. 2015

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A novel ICP MS DNA assay based on lanthanide labels and hybridization chain reaction amplification has been developed. This approach utilizes an enzyme-free amplification and can be operated under mild conditions. The developed assay exhibits desirable sensitivity and is suitable for multiple DNA target analysis because a general DNA hybridization approach is applied to capture target DNA. Considering lanthanide labels and ICP-MS have excellent ability in high-level multiplexing analysis, this approach holds great potential for practical use in quantitative determination of DNA targets for future clinical applications.

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“…As an alternative to the traditional controlled radical polymerization techniques, we have developed an enzymatic approach for the in situ synthesis of thick, dense polymer brushes. We utilize DNA polymerases to synthesize linear DNA molecules by solid‐phase rolling‐circle amplification (RCA) 27–31. First, the oligonucleotide primers are covalently attached to the surface.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling‐Circle Amplification

Barbee

Chandrangsu

Huang

2011

Macromolecular Bioscience

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A method for fabricating DNA polymer brush arrays using photolithography and plasma etching followed by solid-phase enzymatic DNA amplification is reported. After attaching oligonucleotide primers to the surface of a glass coverslip, a thin layer of photoresist is spin-coated on the glass and patterned via photolithography to generate an array of posts in the resist. An oxygen-based plasma is then used to destroy the exposed oligonucleotide primers. The glass coverslip with the primer array is assembled into a microfluidic chip and DNA polymer brushes are synthesized on the oligonucleotide array by rolling-circle DNA amplification. We have demonstrated that the linear polymers can be rapidly synthesized in situ with a high degree of control over their density and length.

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Nucleic acid sensing by regenerable surface-associated isothermal rolling circle amplification

Cited by 12 publications

References 39 publications

Recent developments in nucleic acid identification using solid-phase enzymatic assays

Recent developments in nucleic acid identification using solid-phase enzymatic assays

ICP-MS DNA assay based on lanthanide labels and hybridization chain reaction amplification

Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling‐Circle Amplification

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