Nucleic acid testing and molecular characterization of HIV infections

Zhao, Junpeng; Chang, Le; Wang, Lunan

doi:10.1007/s10096-019-03515-0

Cited by 25 publications

(18 citation statements)

References 130 publications

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“…Molecular testing is more costly due to the need to utilize delicate nucleic acid polymerases that are heat-labile enzymes that drive the amplification reactions needed for sample detection [ 53 ]. At this time, there are no genotyping tests needed due to lack of variation in SARS-CoV-2 that is encountered with other viruses including HIV and HCV and it does not appear that antivirals select for resistant mutants either at this time [ 54 , 55 ].…”

Section: General Virus Testing Approachesmentioning

confidence: 99%

Diagnostic Testing for SARS-CoV-2 Infection

Thomas

Delabat

Andrews

2021

Curr Hepatology Rep

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Purpose of Review Given the rapid development of diagnostic approaches to test for and diagnose infection with SARS-CoV-2, many options are available to assess infection. Multiple established diagnostic companies are now providing testing platforms whereas initially, testing was being performed with simple PCR-based tests using standard laboratory reagents. Recent Findings. Additional testing platforms continue to be developed but challenges with testing, including obtaining testing reagents and other related supplies, are frequently encountered. With time, the testing supply chain will improve and more companies will be providing materials to support these testing efforts. In the USA, the need for rapid assay development and subsequent approval through attainment of emergency use authorization (EUA) has superseded the traditional arduous diagnostic testing approval workflow mandated by the FDA. It is anticipated that the USA will be able to continue to significantly increase its testing capabilities to address this pandemic; however, challenges remain due to the diversity of the performance characteristics of tests being utilized. Summary This review provides an overview of the current diagnostic testing landscape, with pertinent information related to SARS-CoV-2 virology and antibody responses, that is available to diagnose infection.

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Section: General Virus Testing Approachesmentioning

confidence: 99%

Diagnostic Testing for SARS-CoV-2 Infection

Thomas

Delabat

Andrews

2021

Curr Hepatology Rep

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“…Nucleic acid testing (NAT) identifies HIV genetic material and can detect disease earlier than other methods. 17 HIV NAT is now widely available but remains expensive and does not provide rapid results. Combining multiple patient samples ("pooling") enables NAT to be used for mass screening, 18 but this is contingent on a low disease prevalence and the ability to combine many samples without loss of test sensitivity or increased contamination.…”

Section: Assay Technologiesmentioning

confidence: 99%

COVID‐19 and beyond: Lessons learned from emergency department HIV screening for population‐based screening in healthcare settings

et al. 2021

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“…KIYOSHI YASUKAWA 1 , ITARU YANAGIHARA 2 and SHINSUKE FUJIWARA 3 Thermus aquaticus (Taq) in 1976 (10), has become widely used since the discovery of PcR. concerning performances of Taq polymerase, it was initially reported that the activity decreased to 50% at incubation at 95˚C for 1.6 h; the rate of processing was 60-150 nucleotides/sec; and the error rate was 0.38-1.32x10 4 errors/base (11).…”

Section: Alteration Of Enzymes and Their Application To Nucleic Acid mentioning

confidence: 99%

“…This advantage is key to appropriate treatment, prevention, and control of transmission of tuberculosis. In HIV detection, the nucleic acid amplification test is more sensitive and quantitative than other methods based on HIV-1-specific antibody or viral antigens, enabling the detection of HIV-1 at the initial stage of infection and the monitoring of disease progression (1,2).…”

Section: Introductionmentioning

confidence: 99%

Alteration of enzymes and their application to nucleic acid amplification (Review)

2020

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Since the discovery of polymerase chain reaction (PcR) in 1985, several methods have been developed to achieve nucleic acid amplification, and are currently used in various fields including clinical diagnosis and life science research. Thus, a wealth of information has accumulated regarding nucleic acid-related enzymes. In this review, some nucleic acid-related enzymes were selected and the recent advances in their modification along with their application to nucleic acid amplification were described. The discussion also focused on optimization of the corresponding reaction conditions. Using newly developed enzymes under well-optimized reaction conditions, the sensitivity, specificity, and fidelity of nucleic acid tests can be improved successfully. Contents 1. Introduction 2. Thermostabilization of reverse transcriptase 3. creation of the reverse transcriptase activity in thermostable dNA polymerase 4. Use of helicase to increase specificity 5. Fidelity evaluation with NGS 6. Use of recombinase and single-strand binding protein for isothermal DNA amplification 7. Other considerable factors involved in nucleic acid amplification 8. conclusions and future perspectives

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Nucleic acid testing and molecular characterization of HIV infections

Cited by 25 publications

References 130 publications

Diagnostic Testing for SARS-CoV-2 Infection

Diagnostic Testing for SARS-CoV-2 Infection

COVID‐19 and beyond: Lessons learned from emergency department HIV screening for population‐based screening in healthcare settings

Alteration of enzymes and their application to nucleic acid amplification (Review)

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