Nucleic acid testing for blood banks: An experience from a tertiary care centre in New Delhi, India

Agarwal, Nitin; Chatterjee, Kabita; Coshic, Poonam; Borgohain, Mayuri

doi:10.1016/j.transci.2013.03.004

Cited by 43 publications

(37 citation statements)

References 14 publications

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“…and Chaurasia et al . have reported a higher NAT yield of one in 610 and one in 628, respectively . These variabilities in NAT yield rates could be attributed to much larger sample size taken into account in the current study.…”

Section: Discussionmentioning

confidence: 54%

Five years of experience with ID‐NAT at a tertiary care centre in North India: An interdictory step in preventing the transfusion‐transmitted Infections

Kabita

Poonam

Rahul

et al. 2016

ISBT Science Series

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Introduction Technological advancements in transfusion medicine have led to safer blood supply to the community like never before. However, blood safety is a daunting task in India with ~1.25 billion population and high prevalence rate of HIV (0.29%), HBV (2–8%) and HCV (2%) in donor population. This study aims at evaluating the impact of ID‐NAT as an add‐on test on safe blood supply in the current Indian scenario. Materials and Method A retrospective observational study was conducted to analyse the data of 5 years of add‐on NAT at Main Blood Bank, AIIMS, New Delhi. Results 1.84% (3556 of 193 167) units were initially NAT reactive. A total of 3495 units were further tested, of which 79.25% were discriminated (193 HIV, 421 HCV, 2060 HBV and 96 co‐infections). The remaining 13% (456) were repeat non‐reactive and 7.7% (269) could not be discriminated. Overall, NAT yield rate was one in 847, whereas virus‐specific NAT yield rates were one in 96 584, one in 2683, one in 1370 and one in 14 859 for HIV, HCV, HBV and HBV/HCV Co‐infections, respectively. Conclusion Five‐years of ID‐NAT screening interdicted 228 probable TTIs to 684 (assuming 100% component separation) transfusion recipients. Implementation of NAT along with routine serological tests for screening of the blood donations definitely improves the transfusion safety. However, interpretation of NAT reactive results should be adjudged cautiously keeping in mind, the chance occurrence of false‐positive reactivity.

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Section: Discussionmentioning

confidence: 54%

Five years of experience with ID‐NAT at a tertiary care centre in North India: An interdictory step in preventing the transfusion‐transmitted Infections

Kabita

Poonam

Rahul

et al. 2016

ISBT Science Series

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“…The ability to successfully implement ID NAT in India and its impact on interdicting release of infectious units has been demonstrated in a multicenter study with centralized testing[55] and on-site screening studies. [56] Although the argument of affordability of ID NAT has been the premise for promotion of pool testing, the residual risk of the resulting TTIs places a serious burden on the health-care, finances, society and human life. The cost of ART treatment substantially borne by the government could be decreased by improved blood screening to prevent potential HIV TTIs.…”

Section: Comparative Evaluation Of Id Versus Mp Nat Performance With mentioning

confidence: 99%

Factors in enhancing blood safety by nucleic acid technology testing for human immunodeficiency virus, hepatitis C virus and hepatitis B virus

Shyamala

2014

Asian J Transfus Sci

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In the last few decades through an awareness of transfusion transmitted infections (TTI), a majority of countries have mandated serology based blood screening assays for Human immunodeficiency virus (HIV), Hepatitis C virus (HCV), and Hepatitis B virus (HBV). However, despite improved serology assays, the transfusion transmission of HIV, HCV, and HBV continues, primarily due to release of serology negative units that are infectious because of the window period (WP) and occult HBV infections (OBI). Effective mode of nucleic acid technology (NAT) testing of the viruses can be used to minimize the risk of TTIs. This review compiles the examples of NAT testing failures for all three viruses; analyzes the causes for failure, and the suggestions from retrospective studies to minimize such failures. The results suggest the safest path to be individual donation testing (ID) format for highest sensitivity, and detection of multiple regions for rapidly mutating and recombining viruses. The role of blood screening in the context of the donation and transfusion practices in India, the donor population, and the epidemiology is also discussed. World wide, as the public awareness of TTIs increases, as the recipient rights for safe blood are legally upheld, as the possibility to manage diseases such as hepatitis through expensive and prolonged treatment becomes accessible, and the societal responsibility to shoulder the health costs as in the case for HIV becomes routine, there is much to gain by preventing infections than treating diseases.

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“…Also, repeatability and homogeneity of the results can be improved further. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] The nucleic acid extraction device is supplied with matching reagents to automatically extract the nucleic acid sample and, therefore, it can be divided into two categories: First one is a large automatic liquid workstation and the second one is a small automated nucleic acid * Authors to whom correspondence should be addressed. extraction device which uses packed reagents to accomplish extraction and purification process.…”

Section: Introductionmentioning

confidence: 99%

Temperature and Motion Modules of Automatic Nucleic Acid Extractor

Zhu¹,

Wan²,

Wang³

et al. 2016

j nanosci nanotechnol

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An automatic nucleic acid extraction device was developed by combining magnetic separation technology and automatic control technology. It was focused on kinematic positioning of the robotic arm and achieved separation and purification of nucleic acids through the magnet transferred technology. This paper presents a device with mechanical arm positioning system, magnetic separation system, and temperature control system. A touch screen was applied as the man-machine interaction. Also, it was featured with the functions for setting parameters and ultraviolet sterilization. Experimental results showed that the high automation device was stable and reliable with excellent extraction effect.

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Nucleic acid testing for blood banks: An experience from a tertiary care centre in New Delhi, India

Cited by 43 publications

References 14 publications

Five years of experience with ID‐NAT at a tertiary care centre in North India: An interdictory step in preventing the transfusion‐transmitted Infections

Five years of experience with ID‐NAT at a tertiary care centre in North India: An interdictory step in preventing the transfusion‐transmitted Infections

Factors in enhancing blood safety by nucleic acid technology testing for human immunodeficiency virus, hepatitis C virus and hepatitis B virus

Temperature and Motion Modules of Automatic Nucleic Acid Extractor

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