Nucleic Acid Visualization Assay for Middle East Respiratory Syndrome Coronavirus (MERS-CoV) by Targeting the UpE and N Gene

Huang, Pei; Jin, Hongli; Zhao, Yongkun; Li, Entao; Yan, Feihu; Chi, Hang; Wang, Qi; Han, Qiuxue; Mo, Ran; Song, Yumeng; Bi, Jinhao; Cui, Jiao; Li, Wujian; He, Hongbin; Wang, Hongmei; Ma, Aimin; Feng, Na; Wang, Jianzhong; Wang, Tiecheng; Wang, Cuiling; Gao, Yuwei; Xia, Xianzhu; Wang, Hualei

doi:10.22541/au.159368943.32235370

Cited by 1 publication

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to the N gene, RT-RPA was found to be slightly less sensitive to detect other molecular targets of MERS-CoV like UpE and ORF 1a [96] , [117] , [118] . The sensitivity of RT-RPA to detect UpE was ranged from 86 to 98.06% while the sensitivity of RT-RPA to detect ORF 1a was 99% [117] , [118] .…”

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 95%

“…Besides, RT-RPA was demonstrated to have 100% specificity in detecting MER-CoV [96] , [117] . These findings suggested that RT-RPA could serve as a sensitive diagnostic tool to detect MERS-CoV by targeting the N gene [96] , [117] . Compared to the N gene, RT-RPA was found to be slightly less sensitive to detect other molecular targets of MERS-CoV like UpE and ORF 1a [96] , [117] , [118] .…”

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 98%

“…Compared to qRT-PCR, RT-RPA assay could run at a lower temperature like 42°C for 15 minutes (shorter test duration) and it can amplify the specific genetic targets after the enzyme recombinase and the oligonucleotide primers bind to the specific genetic regions [96] , [116] . When RT-RPA was used to detect the N gene of MERS-CoV, it was shown that RT-RPA could detect as low as 1.2 RNA copies in 1 μL of a sample with 100% sensitivity [117] or 10 RNA molecules per reaction with comparable sensitivitiy to qRT-PCR [96] . Besides, RT-RPA was demonstrated to have 100% specificity in detecting MER-CoV [96] , [117] .…”

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 99%

“…When RT-RPA was used to detect the N gene of MERS-CoV, it was shown that RT-RPA could detect as low as 1.2 RNA copies in 1 μL of a sample with 100% sensitivity [117] or 10 RNA molecules per reaction with comparable sensitivitiy to qRT-PCR [96] . Besides, RT-RPA was demonstrated to have 100% specificity in detecting MER-CoV [96] , [117] . These findings suggested that RT-RPA could serve as a sensitive diagnostic tool to detect MERS-CoV by targeting the N gene [96] , [117] .…”

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 99%

See 3 more Smart Citations

Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Chong

Liew

Ong

et al. 2021

Pathology - Research and Practice

View full text Add to dashboard Cite

Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are two common betacoronaviruses, which are still causing transmission among the human population worldwide. The major difference between the two coronaviruses is that MERS-CoV is now causing sporadic transmission worldwide, whereas SARS-CoV-2 is causing a pandemic outbreak globally. Currently, different guidelines and reports have highlighted several diagnostic methods and approaches which could be used to screen and confirm MERS-CoV and SARS-CoV-2 infections. These methods include clinical evaluation, laboratory diagnosis (nucleic acid-based test, protein-based test, or viral culture), and radiological diagnosis. With the presence of these different diagnostic approaches, it could cause a dilemma to the clinicians and diagnostic laboratories in selecting the best diagnostic strategies to confirm MERS-CoV and SARS-CoV-2 infections. Therefore, this review aims to provide an up-to-date comparison of the advantages and limitations of different diagnostic approaches in detecting MERS-CoV and SARS-CoV-2 infections. This review could provide insights for clinicians and scientists in detecting MERS-CoV and SARS-CoV-2 infections to help combat the transmission of these coronaviruses.

show abstract

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 95%

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 98%

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 99%

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 99%

See 2 more Smart Citations

Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Chong

Liew

Ong

et al. 2021

Pathology - Research and Practice

View full text Add to dashboard Cite

show abstract

Nucleic Acid Visualization Assay for Middle East Respiratory Syndrome Coronavirus (MERS-CoV) by Targeting the UpE and N Gene

Cited by 1 publication

References 0 publications

Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Contact Info

Product

Resources

About