Nucleolar protein 4-like has a complex expression pattern in zebrafish embryos

Borah, Supriya; Barrodia, Praveen; Swain, Roy

doi:10.1387/ijdb.150307rs

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…In humans, NOL4 is expressed specifically in the fetal and adult brain and testis, and locates to the nucleoli in cultured cells [50], but their molecular functions remained uncharacterized. Zebrafish has two homologs of NOL4, called Znol4la and Znol4b, which has high amino acid sequence homology to the human NOL4 [51]. Zebrafish embryo expresses Znol4b strongly in the central nervous system including the spinal cord and hindbrain, suggesting that it may have neuronal functions.…”

Section: Mlr1 Mlr2 Interact With Multiple Cofactors Including a Cmentioning

confidence: 99%

Mblk-1 Transcription Factor Family: Its Roles in Various Animals and Regulation by NOL4 Splice Variants in Mammals

Takayanagi-Kiya

Kiya

Kunieda

et al. 2017

IJMS

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Transcription factors play critical roles in regulation of neural development and functions. A transcription factor Mblk-1 was previously reported from a screen for factors possibly important for the higher brain functions of the honeybee. This review first summarizes how Mblk-1 was identified, and then provides an overview of the studies of Mblk-1 and their homologs. Mblk-1 family proteins are found broadly in animals and are shown to affect transcription activities. Studies have revealed that the mammalian homologs can interact with several cofactors and together regulate transcription. Interestingly, a recent study using the mouse homologs, Mlr1 and Mlr2, showed that one of their cofactor proteins, NOL4, have several splice variants with different effects on the transactivation activities of Mlr proteins. These findings suggest that there is an additional layer of the regulation of Mblk-1 family proteins by cofactor splice variants and provide novel insights into our current understanding of the roles of the conserved transcription factor family.

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Section: Mlr1 Mlr2 Interact With Multiple Cofactors Including a Cmentioning

confidence: 99%

Mblk-1 Transcription Factor Family: Its Roles in Various Animals and Regulation by NOL4 Splice Variants in Mammals

Takayanagi-Kiya

Kiya

Kunieda

et al. 2017

IJMS

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“…TWAS genes were also found in liberal loci including C2CD3 (C2 calcium dependent domain containing 3) on 11q13.4 known to play a role in ciliopathies, 28 NUMB (NUMB, endocytic adaptor protein) on 14q24.3 involved in development and cancer, 29 and NOL4L (nucleolar protein 4 like) on 20q11.21 whose function is largely unknown, but previously implicated in leukemia 30 and zebrafish development. 31 For many COPD risk loci, the most likely causal genes were not identified with the current data and will require further investigation. This is consistent with the lower than expected number of variants that colocalized between GWAS on glucose-and insulin-related traits and eQTL in human pancreatic islets and 44 different tissues in the GTEx Portal.…”

Section: Discussionmentioning

confidence: 79%

Leveraging lung tissue transcriptome to uncover candidate causal genes in COPD genetic associations

Obeidat

Lamontagne

Bérubé

et al. 2017

Preprint

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We collated 129 non-overlapping risk loci for chronic obstructive pulmonary disease (COPD) from the GWAS literature. Using recent and complementary integrative genomics approaches, combining GWAS and lung eQTL results, we identified 12 novel COPD loci and corresponding causal genes. In addition, we mapped candidate causal genes for 60 out of the 129 GWASnominated loci as well as for four sub-genome-wide significant COPD risk loci derived from the largest GWAS on COPD. Mapping causal genes in lung tissue represents an important contribution on the genetics of COPD, enriches our biological interpretation of GWAS findings, and brings us closer to clinical translation of genetic associations.All rights reserved. No reuse allowed without permission.

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“…Embryos at different stages [ 26 ] were fixed in 4% PFA and stored in 100% methanol at − 20 °C. Whole-mount in situ hybridization was performed as previously described [ 27 , 28 ]. Riboprobes against zebrafish efhc2 , slc20a1a , trpm7 , slc12a1 , slc12a3 , stc1 , odf3 and pdzk1 were synthesized and used.…”

Section: Methodsmentioning

confidence: 99%

EF-hand domain containing 2 (Efhc2) is crucial for distal segmentation of pronephros in zebrafish

2018

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BackgroundThe blood filtering organ in zebrafish embryos is the pronephros, which consists of two functional nephrons. Segmentation of a nephron into different domains is essential for its function and is well conserved among vertebrates. Zebrafish has been extensively used as a model to understand nephron segmentation during development. Here, we have identified EF-hand domain containing 2 (Efhc2) as a novel component of genetic programme regulating nephron segmentation in zebrafish. Human EFHC2 is a protein with one predicted calcium-binding EF-hand motif and three DM10 domains, whose function is unknown. EFHC2 has been implicated in several brain-related genetic diseases like Turner syndrome and juvenile myoclonic epilepsy. However, there is limited information on its normal physiological function.Resultsefhc2 mRNA is primarily expressed in the pronephros of zebrafish embryos. Other sites of expression include olfactory placode, notochord, otic vesicle, epiphysis and neuromast cells. Morpholino antisense oligonucleotide-mediated knock-down of Efhc2 resulted in defects in pronephros development and function in zebrafish embryos. Efhc2 knock-down leads to expansion of distal early segment of pronephros, whereas, the corpuscle of stannius and distal late segments were reduced. The number of multi-ciliated cells (MCC) that are present in a salt-and-pepper fashion throughout the middle of each nephron and vital for fluid flow were also reduced. It is known that retinoic acid (RA) signaling regulates pronephros segmentation in vertebrates and we show that Efhc2 function is crucial for nephron segmentation in zebrafish. Our data suggests that RA and Efhc2 function independent of each other in pronephros segmentation. However, Efhc2 and RA synergistically regulate MCC development.ConclusionIn this study, we have identified Efhc2 as a regulator of segmentation of the distal part of nephron and pronephros function during zebrafish development.Electronic supplementary materialThe online version of this article (10.1186/s13578-018-0253-z) contains supplementary material, which is available to authorized users.

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Nucleolar protein 4-like has a complex expression pattern in zebrafish embryos

Cited by 9 publications

References 14 publications

Mblk-1 Transcription Factor Family: Its Roles in Various Animals and Regulation by NOL4 Splice Variants in Mammals

Mblk-1 Transcription Factor Family: Its Roles in Various Animals and Regulation by NOL4 Splice Variants in Mammals

Leveraging lung tissue transcriptome to uncover candidate causal genes in COPD genetic associations

EF-hand domain containing 2 (Efhc2) is crucial for distal segmentation of pronephros in zebrafish

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