Nucleoside Diphosphate Kinase Escalates A-to-C Mutations in MutT-Deficient Strains of Escherichia coli

Kapoor, Indu; Emam, Elhassan Ali Fathi; Shaw, Abhirup; Varshney, Umesh

doi:10.1128/jb.00567-19

Cited by 9 publications

(5 citation statements)

References 45 publications

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“…A total of 32 Kan R mutants were collected, 25 that arose from S-P setup, and 7 from the P-P setup. To determine the mutation frequency (MF) accurately, we measured resistance to the antibiotics Rifampicin (Rif) and Kan (Fowler et al, 1994; Kapoor et al, 2019; Miller et al, 1977) in both populations. Compared to P cells, S cells of E. coli exhibited a ~5-fold higher MF (Figure 1E).…”

Section: Resultsmentioning

confidence: 99%

Efflux-linked Accelerated Evolution of Antibiotic Resistance at a Population Edge

Harshey¹,

Bhattacharyya²,

Bhattacharyya

et al. 2022

Preprint

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How antibiotic-resistance mutations survive Darwinian forces in the absence of antibiotics is a long-standing question. We report an unexpected evolutionary phenomenon we call phenotype surfing wherein twin phenotypes - high mutation frequencies and high efflux - segregate at the advancing edge of moving E. coli swarms. These phenotypes are linked: high efflux causes downregulation of specific DNA repair pathways, elevating mutations. We uncovered a genetic network connecting efflux and DNA repair, which we corroborated in a clinical 'resistome' database: genomes with mutations that increase efflux exhibit a significant increase in antibiotic-resistance mutations. Consequently, inhibitors that target efflux decreased evolvability to antibiotic resistance. Modeling shows how a mutator phenotype delivered to the edge by faster motility increases genetic drift, enabling survival and range expansion of mutants.

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Section: Resultsmentioning

confidence: 99%

Efflux-linked Accelerated Evolution of Antibiotic Resistance at a Population Edge

Harshey¹,

Bhattacharyya²,

Bhattacharyya

et al. 2022

Preprint

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“…This process prevents the incorporation of abnormal nucleotides caused by oxidative stress into DNA, which can lead to DNA damage. The deletion of MutT results in a greatly increased mutation rate of AT-CG in E. coli [13]. MazG in Mycobacterium tuberculosis hydrolyzes dUTP, 2-hydroxy-dATP and 8-oxo-dGTP.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of the Expression and Prognosis for DCTPP1 gene in Breast Cancer

Hoang

Bui

Nguyễn³

2021

Asian Pac J Cancer Biol

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Background: Breast cancer is a common malignancy in women. DCTPP1 is a potential target for the development of antitumor drugs, and plays an important role in the process of DNA replication. Aims: To investigate the biological role of DCTPP1 gene, as well as its expression in breast cancer and its relation to patient prognosis. Materials and Methods: Breast cancer data was derived from the TCGA database. Using the UALCAN database, the expression level of DCTPP1 mRNA in breast cancer tissues was investigated. The expression of DCTPP1 in various pathological types of breast cancer was studied using the Human Protein Atlas. UALCAN was also used to investigate the relationship between DCTPP1 gene expression and breast cancer patient prognosis. Bioinformatics studied the proteins related to DCTPP1 expression and their roles in the GeneMANIA and WebGestalt databases. Results: DCTPP1 mRNA was significantly expressed in breast cancer compared to normal breast tissue (P<0.001). DCTPP1 was shown to be highly expressed in breast cancer tissues from different pathological types and stages (P<0.001). The DCTPP1 protein was expressed at a higher frequency in breast cancer than in normal breast tissue. When compared to patients with low DCTPP1 expression, patients with high DCTPP1 expression had a considerably shorter overall survival time. The 20 proteins related to DCTPP1 expression were mostly located in the nucleus and membrane, and were involved in biological regulation, stimulus response, metabolic process, and other processes, according to gene ontology analysis. It plays an important role in protein binding, ion binding, and nucleic acid binding. Conclusion: DCTPP1 is highly expressed in breast cancer, and is associated to a poor prognosis for patients with breast cancer. DCTPP1 may be a potential therapy and intervention target for breast cancer.

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“…All of the enzymes and reaction components needed for GMP manufacture of mRNA are available from commercial providers as synthesized chemicals or bacterially produced, animal componentfree reagents [271]. GMP-grade traceable plasmid DNA, phage polymerases, capping enzymes, and NTPs are available [272]. Other ingredients are pricey or unavailable [272].…”

Section: Therapeutic Considerations and Challengesmentioning

confidence: 99%

“…GMP-grade traceable plasmid DNA, phage polymerases, capping enzymes, and NTPs are available [272]. Other ingredients are pricey or unavailable [272]. As mRNA therapy production increases up, more cost-effective GMP source materials may become available [273].…”

Section: Therapeutic Considerations and Challengesmentioning

confidence: 99%

The use of RNA-based treatments in the field of cancer immunotherapy

Chehelgerdi

2023

Mol Cancer

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Over the past several decades, mRNA vaccines have evolved from a theoretical concept to a clinical reality. These vaccines offer several advantages over traditional vaccine techniques, including their high potency, rapid development, low-cost manufacturing, and safe administration. However, until recently, concerns over the instability and inefficient distribution of mRNA in vivo have limited their utility. Fortunately, recent technological advancements have mostly resolved these concerns, resulting in the development of numerous mRNA vaccination platforms for infectious diseases and various types of cancer. These platforms have shown promising outcomes in both animal models and humans. This study highlights the potential of mRNA vaccines as a promising alternative approach to conventional vaccine techniques and cancer treatment. This review article aims to provide a thorough and detailed examination of mRNA vaccines, including their mechanisms of action and potential applications in cancer immunotherapy. Additionally, the article will analyze the current state of mRNA vaccine technology and highlight future directions for the development and implementation of this promising vaccine platform as a mainstream therapeutic option. The review will also discuss potential challenges and limitations of mRNA vaccines, such as their stability and in vivo distribution, and suggest ways to overcome these issues. By providing a comprehensive overview and critical analysis of mRNA vaccines, this review aims to contribute to the advancement of this innovative approach to cancer treatment.

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Nucleoside Diphosphate Kinase Escalates A-to-C Mutations in MutT-Deficient Strains of Escherichia coli

Cited by 9 publications

References 45 publications

Efflux-linked Accelerated Evolution of Antibiotic Resistance at a Population Edge

Efflux-linked Accelerated Evolution of Antibiotic Resistance at a Population Edge

Comprehensive Analysis of the Expression and Prognosis for DCTPP1 gene in Breast Cancer

The use of RNA-based treatments in the field of cancer immunotherapy

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