Nucleotide clusters in deoxyribonucleic acids. X. Sequences of the pyrimidine oligonucleotides of mouse L-cell satellite DNA

Harbers, Klaus; Harbers, Barbara; Spencer, John H.

doi:10.1016/s0006-291x(74)80490-0

Cited by 10 publications

(3 citation statements)

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“…Information on the fine structure of the satellite repeat units came from an analysis of oligopyrimidine isostichs and oligonucleotides from satellite transcripts (10)(11)(12). The data support the view that the DNA is composed of a basic repeating oligonucleotide and variants thereof.…”

Section: Introductionmentioning

confidence: 74%

“…Still it is clear from the sequence analysis that the Sau96 monomer is not a unique sequence. Comparison of the pyrimidine oligonucleotides in the prototype sequence with those previously determined (10)(11)(12) shows that most of the major tracts that were found at the time are contained in the prototype sequence but that there are a number of minor ones occurring every 300 to 1000 bp which must be derived from diverged variants.…”

Section: G Tg a A A A A T G A G A A A Amentioning

confidence: 80%

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Nudeotide sequence of mouse satellite DNA

1981

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The nucleotide sequence of uncloned mouse satellite DNA has been determined by analyzing Sau96I restriction fragments that correspond to the repeat unit of the satellite DNA. An unambiguous sequence of 234 bp has been obtained. The sequence of the first 250 bases from dimeric satellite fragments present in Sau96I limit digests corresponds almost exactly to two tandemly arranged monomer sequences including a complete Sau96I site in the center. This is in agreement with the hypothesis that a low level of divergence which cannot be detected in sequence analyses of uncloned DNA is responsible for the appearance of dimeric fragments. Most of the sequence of the 5% fraction of Sau96 monomers that are susceptible to TaqI has also been determined and has been found to agree completely with the prototype sequence. The monomer sequence is internally repetitious being composed of eight diverged subrepeats. The divergence pattern has interesting implications for theories on the evolution of mouse satellite DNA.

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