Nucleotide sequence and phylogeny of the tet(L) tetracycline resistance determinant encoded by plasmid pSTE1 from Staphylococcus hyicus

Schwarz, Štefan; Cardoso, Marisa; Wegener, Henrik Caspar

doi:10.1128/aac.36.3.580

Cited by 68 publications

(57 citation statements)

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“…A number of small plasmids carrying antibiotic resistance genes were detected (see Chapter 3). In particular, a plasmid of 11.5 kbp, mediating tetracycline and macrolide/lincosamide resistance [87], was found more often in isolates from diseased pigs, but genes coding for virulence factors were not found to be located on this plasmid. The investigation of 100 S. hyicus isolates for their MIC values of 13 different antimicrobial agents identified novobiocin as the most active compound tested, followed by enrofloxacin, ampicillin and ceftiofur [102].…”

Section: Staphylococcus Hyicusmentioning

confidence: 95%

Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

et al. 2001

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-Besides their role as commensals on the skin and mucosal surfaces, staphylococci may be involved in a wide variety of diseases in animals. Staphylococcal infections in animals are mainly treated with antimicrobial agents and as a consequence, staphylococci from animal sources have developed and/or acquired resistance to the respective antimicrobial agents. Resistance statistics obtained from national monitoring programmes on staphylococci from cattle and pigs, but also from surveillance studies on staphylococci involved in diseases in dogs are reported and reviewed with regard to their comparability. This review mainly focusses on the genetic basis of antimicrobial resistance in staphylococci of animal origin. Particular attention is paid to resistance to those antimicrobial agents which are most frequently used in veterinary medicine, but also to antimicrobial agents, such as chloramphenicol and mupirocin, which are used in specific cases for the control of staphylococcal infections in pets and companion animals. In addition, plasmids and transposons associated with the respective resistance properties and their ways of spreading between members of the same or different staphylococcal species, but also between staphylococci and other gram-positive bacteria, are described.antimicrobial resistance / Staphylococcus / animal origin / epidemiology / horizontal gene transfer Vet. Res. 32 (2001) 341

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Section: Staphylococcus Hyicusmentioning

confidence: 95%

Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

et al. 2001

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“…topology of the Tet(K) protein There are 9 acidic residues conserved throughout the sequences of Tet(K) and Tet(L) proteins isolated from six different bacterial species [26]. The distribution of these acidic residues is shown in Fig.…”

Section: Distribution Of Conserved Acidic Residues and Putativementioning

confidence: 99%

“…1. Among them, Asp-200 is conserved except for Tet(L) encoded by pSTE1, in which Asp is replaced by Gly [26]. The Tet(L) protein encoded by a chromosome of B. subtilis has Asp-345 in place of Glu-345 [26].…”

Section: Distribution Of Conserved Acidic Residues and Putativementioning

confidence: 99%

“…Among them, Asp-200 is conserved except for Tet(L) encoded by pSTE1, in which Asp is replaced by Gly [26]. The Tet(L) protein encoded by a chromosome of B. subtilis has Asp-345 in place of Glu-345 [26]. The other 7 acidic residues are completely conserved in all of the Tet(K) and Tet(L) proteins [26].…”

Section: Distribution Of Conserved Acidic Residues and Putativementioning

confidence: 99%

“…The Tet(L) protein encoded by a chromosome of B. subtilis has Asp-345 in place of Glu-345 [26]. The other 7 acidic residues are completely conserved in all of the Tet(K) and Tet(L) proteins [26]. The conserved aspartic acid residues are Asp-39, Asp-74, Asp-200, and Asp-318.…”

Section: Distribution Of Conserved Acidic Residues and Putativementioning

confidence: 99%

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Transmembrane glutamic acid residues play essential roles in the metal‐tetracycline/H⁺ antiporter of Staphylococcus aureus

Fujihira¹,

Kimura²,

Shiina³

et al. 1996

FEBS Letters

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; however, it has no transmembrane aspartyl residue. On the other hand, Tet(K) has three glutamyl residues, Glu-30, Glu-152 and Glu-397, in the putative transmembrane regions. In the present work, tet(K) gene was expressed in Escherichia coli and the transport activity was measured in everted membrane vesicles. When these glutamyl residues were replaced with Gin, the tetracycline transport activity was almost completely lost, indicating the important roles of these residues in Tet(K). In the case of Glu-397, even the charge-conserved mutation to Asp caused complete loss of the activity. On the other hand, the mutation of Glu-30 and Glu-152 to Asp resulted in significant retention of transport activity. These results are similar to those on the mutation of the three transmemhrane aspartyl residues in Tet(B), indicating that the transmembrane glutamyl residues in Tet(K) play roles similar to those of the transmemhrane aspartyl residues in Tet(B).

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Resistance to Other Agents

Strahilevitz

Hooper

2009

Staphylococci in Human Disease

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Nucleotide sequence and phylogeny of the tet(L) tetracycline resistance determinant encoded by plasmid pSTE1 from Staphylococcus hyicus

Abstract: The nucleotide sequence of the tetracycline resistance (tet) gene and its regulatory region, encoded by the plasmid pSTE1 from Staphylococcus hyicus, was determined.

Cited by 68 publications

References 50 publications

Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

Transmembrane glutamic acid residues play essential roles in the metal‐tetracycline/H⁺ antiporter of Staphylococcus aureus

Resistance to Other Agents

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Nucleotide sequence and phylogeny of the tet(L) tetracycline resistance determinant encoded by plasmid pSTE1 from Staphylococcus hyicus

Abstract: The nucleotide sequence of the tetracycline resistance (tet) gene and its regulatory region, encoded by the plasmid pSTE1 from Staphylococcus hyicus, was determined.

Cited by 68 publications

References 50 publications

Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

Transmembrane glutamic acid residues play essential roles in the metal‐tetracycline/H+ antiporter of Staphylococcus aureus

Resistance to Other Agents

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Transmembrane glutamic acid residues play essential roles in the metal‐tetracycline/H⁺ antiporter of Staphylococcus aureus