Nucleotide Sequence of Rice Dwarf Phytoreovirus Genome Segment 2: Completion of Sequence Analyses of Rice Dwarf Virus

Abstract

The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 (S2) was determined to be 3,512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363. The encoded polypeptide was predicted to have 1,116 residues with a relative molecular weight of 123 kD. Comparison of S2 of two isolates showed they had identical lengths and 97 and 98.3% nucleotide and amino acid sequence identities, respectively. A search of the Swiss-Prot data base (R 22.0) failed to find any proteins with significant homology to the S2-encoded protein. Determination of the nucleotide sequence of the S2 has completed the sequence determination of the genome of rice dwarf virus. Homology searches made for proteins encoded by each of the genomic segments showed that the polypeptide encoded by SI 1 has similarity to histone HI protein and VP6 of blue tongue virus, indicating it might possess nucleic acid binding properties.

Rice Dwarf Phytoreovirus Segment S6-Encoded Nonstructural Protein Has a Cell-to-Cell Movement Function

“…The sequences of two isolates have been determined (43,51). The genome of RDV is composed of 12 separate segments of double-stranded RNA (3).…”

Plant viruses and viroids in Japan

“…In Japan, complete nucleotide sequences of RDV genome segments were determined from 1987 to 1994 (Uyeda et al 1987(Uyeda et al , 1994. The analysis led RDV to be the first plant reovirus to be sequenced.…”

Molecular analysis of rice ragged stunt oryzavirus segment 9 and sequence conservation among isolates from Thailand and India

“…reoviruses: wound tumor virus (WTV) genome segments 4 to 12 [23], rice dwarf virus (RDV) genome segments 1 to 12 [28][29][30][31][32]35], rice gall dwarf virus (RGDV) genome segments 8 to 10 [14,22], maize rough dwarf virus (MRDV) genome segment 6 [18], and rice black-streaked dwarf virus (RBSDV) genome segments 7, 8 and 10 [1,33]. To date the only nucleotide sequence information about RRSV is confined to the 5' and 3' termini (5' G A U A A A ---and ---G U G C 3') which was obtained by direct R N A sequencing [39].…”