Nucleotide sequence of the 3' terminal region of the LEU2 gene from Saccharomyces cerevisiae

Froman, Byron E.; Tait, Robert C.; Rodriguez, Raymond L.

doi:10.1016/0378-1119(84)90218-x

Cited by 17 publications

(6 citation statements)

References 14 publications

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“…In recent years restriction endonuclease fragment pattern polymorphisms (RFLPs) of several DNA regions have proven to be very useful for differentiation of yeast strains (2,10,13,16,18,19,20,27,30,35,39,40,41,42,43,44,45,48,55). In the present study four DNA regions have been screened, namely: RDN1 (7,15,47) on chromosome XII (46), HIS4 (21,26,27) and LEU2 (3,5,14,22,30,59) genes on chromosome III (38), and the Ty element regions homologous to Tyl from S. cerevisiae genetic standard strains (19,20,48). RDN1 encodes the 18S, 5.8S, 25S, and 5S cytosolic ribosomal RNA molecules (7,47,54).…”

Section: Introductionmentioning

confidence: 97%

DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

Pedersen

1986

Carlsberg Res. Commun.

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30 bottom fermenting strains, 13 hybrid lager strains, 11 top fermenting strains. In addition, 24 other yeast species and strains have been analysed in this study. Four DNA regions have been monitored by restriction endonuclease fragment pattern polymorphisms. The regions analysed are: RDN1 (encoding cytosolic ribosomal RNA molecules) located on chromosome XII in S. cerevisiae, HIS4 (histidine 4) and LEU2 (leucine 2) both located on chromosome III and the Ty elements which are distributed at different positions in the genome ofS. cerevisiae. Seven Ty fragment patterns have been detected in the bottom fermenting strains. Patterns I, II, IIa, lib, and llc are present in lager strains while pattern III and IV are found in S. carlsbergensis bottom fermenting strain No. I and in S. monacensis, respectively. Hybridization of the Tyl p14 probe from S. cerevisiae to OFAGE separated chromosomes from bottom fermenting strains show that at least five Ty elements are present in the genomes of the lager strains, at least five in S. carlsbergensis bottom fermenting strain No. I and at least three in S. monacensis. From the restriction fragment patterns and the karyotypes of the bottom fermenting strains it is suggested that these strains are closely related and different from the S. bayanus, S. pastorianus, and S. uvarum group as well as from the S. cerevisiae strains. In spite of their differences in Ty element patterns the lager strains are so homogenous that most likely they all originate from a single strain which is related to S. carlsbergensis and S. monacensis type strains. In contrast to the bottom fermenting strains there is a large variability among the top fermenting strains, especially with regard to the LEU2 gene and Ty elements. It seems that S. bayanus, S. inusitatus, S. pastorianus, S. validus, and S. uvarum are closely related, and none of the studied strains from these taxons contained DNA which hybridized to the Tyl probe from S. cerevisiae. This is a clear difference from the DNA of bottom fermenting strains which consistently hybridized strongly to the Tyl probe.Abbreviations: kb = kilo base pairs; OFAGE = orthogonal field alternation gel electrophoresis; SSC = 0.15 M-NaCI, 15 mM-Na citrate; TBE = 0.089 M-Tris-borate, 0.089 M-boric acid, 0.002 M-EDTA; Tris = tris-(hydroxymethyl)-amino methane Springer-Verlag

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Section: Introductionmentioning

confidence: 97%

DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

Pedersen

1986

Carlsberg Res. Commun.

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“…The sequencing of one end of the clone revealed a sequence that had 69% homology to S. cerevisiae NFS1 ( SPL1 , YCL017c), a gene encoding an essential protein involved in tRNA splicing (Froman et al , 1984). In S. cerevisiae , downstream from the NFS1 gene is LEU2 gene coding for β‐isopropylmalate dehydrogenase.…”

Section: Resultsmentioning

confidence: 99%

Cloning, sequencing and application of the LEU2 gene from the sour dough yeast Candida milleri

Turakainen

Korhola

2005

Yeast

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We have cloned by complementation in Saccharomyces cerevisiae and sequenced a LEU2 gene from the sour dough yeast Candida milleri CBS 8195 and studied its chromosomal location. The LEU2 coding sequence was 1092 nt long encoding a putative β-isopropylmalate dehydrogenase protein of 363 amino acids. The nucleotide sequence in the coding region had 71.6% identity to S. cerevisiae LEU2 sequence. On the protein level, the identity of C. milleri Leu2p to S. cerevisiae Leu2p was 84.1%. The DNA sequence of the C. milleri LEU2 gene was submitted to GenBank and assigned Accession No. AY571337. The CmLEU2 DNA probe hybridized to one to three chromosomal bands and two or three BamHI restriction fragments in C. milleri but did not give any signal to chromosomes or restriction fragments of C. albicans, S. cerevisiae, S. exiguus or Torulaspora delbrueckii. Using CmLEU2 probe for DNA hybridization makes it easy to quickly identify C. milleri among other sour dough yeasts.

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“…The fragment of ZrNFS1 is 1162 bp long (nt 1469–2630), and it codes for a polypeptide of 387 aa (the complete S.cerevisiae Nfs1p is 497 aa long). The LEU2 and NFS1 orientation is conserved between both yeasts, but the intergenic region is much shorter in Z.rouxii than in S. cerevisiae (150 and 360 nt, respectively; Froman et al ., 1984). Similar orientation between adjacent LEU2 and NFS1/SPL1 genes has been found also in other yeast species, e.g.…”

Section: Resultsmentioning

confidence: 99%

Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a β‐isopropylmalate dehydrogenase

Sychrová

2001

Yeast

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A DNA fragment carrying the LEU2 gene of osmotolerant yeast Zygosaccharomyces rouxii was isolated. The sequenced DNA fragment (2630 bp) contained two ORFs; one of them (1086 bp long, predicting a protein of 362 amino acids) shared a high degree of similarity with LEU2 genes of other yeast species. The cloned DNA fragment fully complemented the leu2 mutations of Saccharomyces cerevisiae and Z. rouxii. The

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Nucleotide sequence of the 3' terminal region of the LEU2 gene from Saccharomyces cerevisiae

Cited by 17 publications

References 14 publications

DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

Cloning, sequencing and application of the LEU2 gene from the sour dough yeast Candida milleri

Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a β‐isopropylmalate dehydrogenase

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