Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4 kbp EcoRI-I and -R (Polyhedrin gene) region

Possee, Robert D.; Sun, Tai-ping; Howard, Stephen C.; Ayres, Martin D.; Hill-Perkins, M; Gearing, Katharine L.

doi:10.1016/0042-6822(91)90770-c

Cited by 106 publications

(57 citation statements)

References 40 publications

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“…We analyzed the 3= PAS of AcMNPV transcripts using a 3= RACE Illumina sequencing protocol, as described in Materials and Methods. The protocol for identifying poly(A) sites was validated by comparing our results with a variety of previously mapped polyadenylation sites (63)(64)(65)(66)(67)(68), and the PAS mapped by Illumina sequencing (see Table S4 in the supplemental material) corresponded closely to sites previously mapped by S1 nuclease protection and/or 3= RACE. For this analysis, we analyzed mRNAs isolated at times ranging from 6 to 48 h p.i.…”

Section: Resultsmentioning

confidence: 60%

The Transcriptome of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Cells

Chen

Zhong

Fei

et al. 2013

J Virol

166

225

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Section: Resultsmentioning

confidence: 60%

The Transcriptome of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Cells

Chen

Zhong

Fei

et al. 2013

J Virol

166

225

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“…AcNPV strain C6 (11) and AcRP5ßgal virus (Ribeiro BM, unpublished results) were used to infect Spodoptera frugiperda (IPLB-SF-21) cells grown in TC100 medium supplemented with 5% fetal calf serum. Virus was titrated by the plaque assay (12).…”

Section: Virus Cells and Insectsmentioning

confidence: 99%

Construction of occluded recombinant baculoviruses containing the full-length cry1Ab and cry1Ac genes from Bacillus thuringiensis

Ribeiro

Crook

1998

Braz J Med Biol Res

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The administration of baculoviruses to insects for bioassay purposes is carried out, in most cases, by contamination of food surfaces with a known amount of occlusion bodies (OBs). Since per os infection is the natural route of infection, occluded recombinant viruses containing crystal protein genes (cry1Ab and cry1Ac) from Bacillus thuringiensis were constructed for comparison with the baculovirus prototype Autographa californica nucleopolyhedrovirus (AcNPV). The transfer vector pAcUW2B was used for construction of occluded recombinant viruses. The transfer vector containing the crystal protein genes was cotransfected with linearized DNA from a non-occluded recombinant virus. The isolation of recombinant viruses was greatly facilitated by the reduction of background "wild type" virus and the increased proportion of recombinant viruses. Since the recombinant viruses containing full-length and truncated forms of the crystal protein genes did not seem to improve the pathogenicity of the recombinant viruses when compared with the wild type AcNPV, and in order to compare expression levels of the full-length crystal proteins produced by nonoccluded and occluded recombinant viruses the full-length cry1Ab and cry1Ac genes were chosen for construction of occluded recombinant viruses. The recombinant viruses containing full-length and truncated forms of the crystal protein genes did not seem to improve its pathogenicity but the size of the larvae infected with the recombinant viruses was significantly smaller than that of larvae infected with the wild type virus.

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“…Several additional minor proteins have also been identified to be associated with the nucleocapsid, including PCNA (2), VP1054 (31), FP25 (4,18,35,48), VLF-1 (22,27,29,42,43,50), VP80 (21,25,30), and P24 (47). PP78/83, VP1054, and VLF-1 have been reported to be required for nucleocapsid assembly (31,34,43). Recently, EXON0 (ORF141) (10,11) and AC142 (26) have also been shown to be nucleocapsid proteins, but neither EXON0 nor AC142 is required for nucleocapsid assembly.…”

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confidence: 99%

Autographa californica Multiple Nucleopolyhedrovirus 38K Is a Novel Nucleocapsid Protein That Interacts with VP1054, VP39, VP80, and Itself

Liang

Kan

et al. 2008

J Virol

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It has been shown that the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) 38K (ac98) is required for nucleocapsid assembly. However, the exact role of 38K in nucleocapsid assembly remains unknown. In the present study, we investigated the relationship between 38K and the nucleocapsid. Western blotting using polyclonal antibodies raised against 38K revealed that 38K was expressed in the late phase of infection in AcMNPV-infected Spodoptera frugiperda cells and copurified with budded virus (BV) and occlusionderived virus (ODV). Biochemical fractionation of BV and ODV into the nucleocapsid and envelope components followed by Western blotting showed that 38K was associated with the nucleocapsids. Immunoelectron microscopic analysis revealed that 38K was specifically localized to the nucleocapsids in infected cells and appeared to be distributed over the cylindrical capsid sheath of nucleocapsid. Yeast two-hybrid assays were performed to examine potential interactions between 38K and nine known nucleocapsid shell-associated proteins (PP78/83, PCNA, VP1054, FP25, VLF-1, VP39, BV/ODV-C42, VP80, and P24), three non-nucleocapsid shell-associated proteins (P6.9, PP31, and BV/ODV-E26), and itself. The results revealed that 38K interacted with the nucleocapsid proteins VP1054, VP39, VP80, and 38K itself. These interactions were confirmed by coimmunoprecipitation assays in vivo. These data demonstrate that 38K is a novel nucleocapsid protein and provide a rationale for why 38K is essential for nucleocapsid assembly.

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Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4 kbp EcoRI-I and -R (Polyhedrin gene) region

Cited by 106 publications

References 40 publications

The Transcriptome of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Cells

The Transcriptome of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Cells

Construction of occluded recombinant baculoviruses containing the full-length cry1Ab and cry1Ac genes from Bacillus thuringiensis

Autographa californica Multiple Nucleopolyhedrovirus 38K Is a Novel Nucleocapsid Protein That Interacts with VP1054, VP39, VP80, and Itself

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