Nucleotide sequence of the p39-capsid gene region of the Lymantria dispar nuclear polyhedrosis virus

Bjornson, Rebecca M.; Rohrmann, George F.

doi:10.1099/0022-1317-73-6-1505

Cited by 15 publications

(7 citation statements)

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“…Recently, the complete nucleotide sequence of the AcMNPV has been reported (Ayres et al, 1994). Although several genes that are present in both LdMNPV and AcMNPV have been identified [polyhedrin (Smith et al, 1988), p39 (Bjornson & Rohrmann, 1992b), vPK (Bischoff & Slavicek, 1994), egt (Riegel et aL, 1994) and D N A polymerase (Bjornson et al, 1992)], there is no G22 gene homologue present in AcMNPV.…”

Section: T a T T A T A A C T T T T A T T T C C T C A T T G T A A A A mentioning

confidence: 99%

“…A number of LdMNPV genes that are also present in D. S. Bischoff and J. M. Slavicek AcMNPV have been identified and characterized, including ecdysteroid UDP-glucosyl transferase (Riegel et al, 1994), DNA polymerase (Bjornson et al, 1992), polyhedral envelope (Bjornson & Rohrmann, 1992a), polyhedrin (Smith et al, 1988), p39 nucleocapsid (Bjornson & Rohrmann, 1992b) and viral protein kinase (Bischoff & Slavicek, 1994).…”

Section: Fswa/s-jslavicek/ou-s24lo5a@mhsattmailcommentioning

confidence: 99%

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Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus

Bischoff

Slavicek

1995

Journal of General Virology

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The Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) gene encoding G22 was cloned and sequenced. The G22 gene codes for a 191 amino acid protein with a predicted M r of 22000. Expression of G22 in a rabbit reticulocyte system generated a protein with an M r of 24000, in close agreement with the molecular mass predicted from the nucleotide sequence. G22 is not significantly homologous to any known protein, nor is a G22 homologue present in the Autographa californica MNPV (AcMNPV). Temporal expression studies indicated that the G22 gene was transcribed at readily detectable levels in the presence of cycloheximide. Transcripts were detected immediately after the virus adsorption period and throughout the infection cycle. The early transcriptional start sites of G22 map to a sequence that resembles a subset of RNA polymerase II promoters/start sites that are found upstream of Drosophila melanogaster developmental and retrotransposon genes which lack TATA box motifs. Several consensus late baculovirus promoter/ mRNA start site sequences (ATAAG) were identified upstream of the G22 gene start codon.

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Section: T a T T A T A A C T T T T A T T T C C T C A T T G T A A A A mentioning

confidence: 99%

Section: Fswa/s-jslavicek/ou-s24lo5a@mhsattmailcommentioning

confidence: 99%

Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus

Bischoff

Slavicek

1995

Journal of General Virology

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“…These probes were individually hybridized to the Northern blots and washed according to the phosphate buffer procedure of Mahmoudi and Lin (1989). To investigate temporal transcription of the DNA polymerase gene, a similar time course was set up using only LdMNPV isolate A21-2 and RNA was harvested at 12,14,16,18,20,22, and 24 h p.i.. DNA polymerase gene transcripts were detected using a NdeI to BgllI 3.0 kb fragment from the genomic region of 87 to 90 kb that contains most of the LdMNPV DNA polymerase gene (Bjornson and Rohrmann, 1992b) as a probe that was labeled by nick-translation with [e_32p] dCTP (NEN). DNA polymerase gene transcripts were also detected by reverse transcription and subsequent polymerase chain reaction (PCR) amplification according to the manufacturers protocol (Perkin Elmer).…”

Section: Measuring Rna Expressionmentioning

confidence: 99%

“…RNA was separated on a 1.2% agarose gel containing formaldehyde, and the RNA was then transferred to nitrocellulose. Blots were probed with three different LdMNPV probes: G22, an immediate early gene (Bischoff and Slavicek, 1995); p39 capsid, a late gene (Thiem and Miller, 1989;Bjornson and Rohrmann, 1992b); and polyhedrin, a hyper-expressed late gene (Smith et al, 1988;Friesen and Miller, 1985). Strand-specific oligonucleotides were end-labeled with [~-32p]ATP (NEN), and used as probes to detect G22, p39 capsid, and polyhedrin gene transcripts.…”

Section: Measuring Rna Expressionmentioning

confidence: 99%

“…The viral genome has been mapped with restriction endonucleases (Smith et al, 1988;Riegel et al, 1994), and transcription and translation maps have been generated (Slavicek, 1991). A number of genes that have been previously identified in the AcMNPV including the ecdysteroid UDP-glucosyl transferase (Riegel et al, 1994), DNA polymerase (Bjornson et al, 1992), polyhedral envelope protein (Bjornson and Rohrmann, 1992a), polyhedrin (Smith et al, 1988), p39 nucleocapsid (Bjornson and Rohrmann, 1992b), and viral protein kinase (Bischoff and Slavicek, 1994), have been located and characterized in the LdMNPV. Even though several LdMNPV genes have been characterized at the structural level the life cycle of this virus had not been detailed.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the replication cycle of the Lymantria dispar nuclear polyhedrosis virus

Riegel

Slavicek

1997

Virus Research

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The life cycle of the Lymantria dispar nuclear polyhedrosis virus (LdMNPV) was characterized through analysis of budded virus (BV) release, the temporal formation of polyhedra, the temporal transcription pattern of representative early, late, and hyper-expressed late genes, and the onset of DNA replication in the Ld652Y cell line. Transcripts from the LdMNPV immediate early gene G22 were detected 4 h post infection (h p.i.). The late and hyper-expressed late p39 capsid and polyhedrin genes were initially transcribed at approximately 20 and 24 h p.i., respectively. Viral DNA replication initiated at approximately 18-20 h p.i. Budded virus was released from infected cells between 24 and 36 h p.i., and polyhedra were first detected at approximately 48 h p.i. © 1997 Elsevier Science B.V.

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Sequence and Analysis of the Genome of a Baculovirus Pathogenic forLymantria dispar

et al. 1999

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The genome of the Lymantria dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV) was sequenced and analyzed. It is composed of 161,046 bases with a G + C content of 57.5% and contains 163 putative open reading frames (ORFs) of >/=150 nucleotides. Homologs were found to 95 of the 155 genes predicted for the Autographa californica MNPV (AcMNPV) genome. More than 9% of the LdMNPV genome was occupied by 16 repeated genes related to AcMNPV ORF2. Readily identifiable homologs of several genes that have been reported to play important roles in the AcMNPV life cycle are not present; these include ie-2, a transcriptional transactivator, and gp64, a major envelope glycoprotein of the nonoccluded form of the virus. A number of genes lacking in AcMNPV but present in other baculoviruses were identified; these include two viral enhancing factor homologs, a second copy of a conotoxin-like gene, and a dutpase homolog. Although a single gene predicted to encode a large subunit of ribonucleotide reductase was found, two different copies of the small subunit gene were present. In addition, homologs of genes not previously reported for baculoviruses were identified, including a predicted protein with homology to DNA ligases and another that has motifs most closely related to a yeast mitochondrial helicase. Thirteen homologous regions (hrs) containing 54 repeated sequences that include 30-bp imperfect palindromes were identified. The imperfect palindromes are related to those from other baculoviruses.

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Nucleotide sequence of the p39-capsid gene region of the Lymantria dispar nuclear polyhedrosis virus

Cited by 15 publications

References 2 publications

Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus

Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus

Characterization of the replication cycle of the Lymantria dispar nuclear polyhedrosis virus

Sequence and Analysis of the Genome of a Baculovirus Pathogenic forLymantria dispar

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