Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis

Moran, Charles P.; Lang, Naomi; LeGrice, Stuart F. J.; Lee, Gloria; Stephens, M. A.; Sonenshein, Abraham L.; Pero, Janice; Losick, Richard

doi:10.1007/bf00729452

Cited by 776 publications

(461 citation statements)

References 37 publications

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“…The mfd22 mutation did not affect the regulation of the HUT643 lacZ fusion in response to carbon availability ( Table 2). The HUT924 lacZ fusion, which contains the hut cre centred 161.5 bp downstream of tms promoter transcriptional start site (Moran et al, 1982), was used to examine CCR mediated solely at the hut cre. ␤-Galactosidase expression from the HUT924 lacZ fusion was repressed 14-fold by glucose in the wild-type strain, but only 5.3-fold in the mfd mutant (Table 2).…”

Section: Resultsmentioning

confidence: 99%

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Transcription–repair coupling factor is involved in carbon catabolite repression of the Bacillus subtilis hut and gnt operons

Zalieckas

Wray

Ferson

et al. 1998

Molecular Microbiology

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SummaryA Bacillus subtilis mutant that partially relieves carbon catabolite repression (CCR) of the hut operon was isolated by transposon mutagenesis. Characterization of this mutant revealed that the transposon had inserted into the gene, mfd, that encodes transcription-repair coupling factor. The Mfd protein is known to promote strand-specific DNA repair by displacing RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the DNA damage site. A set of transcriptional lacZ fusions was used to demonstrate that the mfd mutation relieves CCR of hut and gnt expression at the cis-acting cre sequences located downstream of the transcriptional start site but does not affect CCR at sites located at the promoters. CCR of the amyE and bglPH genes, which contain cre sequences that overlap their promoters, is not altered by the mfd mutation. These results support a model in which the Mfd protein displaces RNA polymerase stalled at downstream cre sites that function as transcriptional roadblocks and reveal a new role for Mfd in cellular physiology.

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Section: Resultsmentioning

confidence: 99%

“…Plasmid pHUT902 contains the tms promoter (Moran et al, 1982) cloned into the polylinker region of pALTER-1 (Promega Corp.). The TMS922 lacZ fusion contains the tms promoter fragment from pHUT902 cloned into pSFL7.…”

Section: Dna Cloning and Plasmid Constructionsmentioning

confidence: 99%

Transcription–repair coupling factor is involved in carbon catabolite repression of the Bacillus subtilis hut and gnt operons

Zalieckas

Wray

Ferson

et al. 1998

Molecular Microbiology

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“…The transcription start sites of the cry35-like operon mapped to 36 and 37 nt upstream from the putative start codon of the cry35-like gene (Figure 3c; Supplementary Figure S5B). The À 10 region of the two promoters resembles that recognized by the vegetative sigma A factor (Moran et al, 1982). In sharp contrast, the À 35 regions did not fit the consensus region recognized by this sigma factor (TTGCATA).…”

Section: Phenotypic and Genetic Characterization Of Lm1212mentioning

confidence: 83%

Division of labour and terminal differentiation in a novel Bacillus thuringiensis strain

et al. 2014

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A major challenge in bacterial developmental biology has been to understand the mechanisms underlying cell fate decisions. Some differentiated cell types display cooperative behaviour. Cooperation is one of the greatest mysteries of evolutionary biology and microbes have been considered as an excellent system for experimentally testing evolution theories. Bacillus thuringiensis (Bt) is a spore-forming bacterium, which is genetically closely related to B. anthracis, the agent of anthrax, and to B. cereus, an opportunistic human pathogen. The defining feature that distinguishes Bt from its relatives is its ability to produce crystal inclusions in the sporulating cells. These toxins are solubilized after ingestion and are cooperative public goods in insect hosts. In this study, we describe a Bt strain LM1212 that presents the unique ability to terminally differentiate into crystal producers and spore formers. Transcriptional analysis based on lacZ and gfp reporter genes suggested that this phenotype is the consequence of a new type of cell differentiation associated with a novel regulation mode of cry gene expression. The differentiating crystal-producer phenotype has higher spore productivity than a typical Bt strain and is better able to compete with Cry toxin null 'cheaters'. Potentially, this division of labour provides additional fitness benefits in terms of spore viability or durability of Cry toxin.

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“…--------- probably contains the spoIIA promoter together with an upstream AT-rich region like that found 5' of other Bacillus operons (Moran et al, 1982). Sequences corresponding to canonical -35 and -10 regions are, however, not immediately obvious.…”

Section: Gcgttccgtttttttgt Ttttttgtmentioning

confidence: 82%

Nucleotide Sequence of the Bacillus licheniformis Homologue of the Sporulation Locus spoIIA of Bacillus subtilis

Yudkin

Appleby²,

Smith³

1989

Microbiology

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The spoIIA locus of Bacillus licheniformis was cloned into the phage vector 4105J9; selection was based on the ability of the clone to complement mutations in both the first and the last of the spoIIA genes of B. subtilis. The B . licheniformis DNA was subcloned into M13 and sequenced; it includes three genes of identical lengths to those of B. subtilis. The average interspecies difference in nucleotide sequence is 24%; C occurs at the third position of codons 55% more often in the B. fichenvormis than in the B . subtilis sequence. The average difference in predicted amino acid sequence is 11 %, but the distribution of these differences is far from random, and there are several long stretches of amino acid sequence that are identical in the two organisms. The distribution of non-conservative amino acid changes between the species is also strikingly non-random; no such changes are found in those regions in which missense mutations are known in B. subtilis. Each species has an open reading frame 5' of the spoIIAA gene, but the predicted amino acid sequence, and the distribution of differences between the two species in both nucleotides and predicted amino acids, suggest that these open reading frames are not expressed. Both species have regions 3' of the open reading frames which resemble rhoindependent terminators of transcription, but that in B. licheniformis is longer and could form a more elaborate secondary structure than that in B. subtifis.

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Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis

Cited by 776 publications

References 37 publications

Transcription–repair coupling factor is involved in carbon catabolite repression of the Bacillus subtilis hut and gnt operons

Transcription–repair coupling factor is involved in carbon catabolite repression of the Bacillus subtilis hut and gnt operons

Division of labour and terminal differentiation in a novel Bacillus thuringiensis strain

Nucleotide Sequence of the Bacillus licheniformis Homologue of the Sporulation Locus spoIIA of Bacillus subtilis

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