Numerical and Experimental Millimeter-Wave Dosimetry for <i>In Vitro</i> Experiments

Sauleau, Ronan; Dréan, Yves Le; Алексеев, С. И.; Ziskin, Marvin C.

doi:10.1109/tmtt.2008.2006797

Cited by 36 publications

(24 citation statements)

References 22 publications

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“…Potential target genes of MMW radiation still remain to be identified. Since MMW are essentially absorbed by the superficial layers of skin [Zhadobov et al, 2008], the main purpose of this study was to perform a large-scale genomic analysis of primary human keratinocytes after 1, 6, or 24 h exposure to MMW at 60.4 GHz.…”

Section: Mmw At Intermediate Pd (5-15 Mw/cmmentioning

confidence: 99%

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Quément

Nicolaz

Desmots

et al. 2011

Bioelectromagnetics

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The main purpose of this study is to investigate potential responses of skin cells to millimeter wave (MMW) radiation increasingly used in the wireless technologies. Primary human skin cells were exposed for 1, 6, or 24 h to 60.4 GHz with an average incident power density of 1.8 mW/cm(2) and an average specific absorption rate of 42.4 W/kg. A large-scale analysis was performed to determine whether these exposures could affect the gene expression. Gene expression microarrays containing over 41,000 unique human transcript probe sets were used, and data obtained for sham and exposed cells were compared. No significant difference in gene expression was observed when gene expression values were subjected to a stringent statistical analysis such as the Benjamini-Hochberg procedure. However, when a t-test was employed to analyze microarray data, 130 transcripts were found to be potentially modulated after exposure. To further quantitatively analyze these preselected transcripts, real-time PCR was performed on 24 genes with the best combination of high fold change and low P-value. Five of them, namely CRIP2, PLXND1, PTX3, SERPINF1, and TRPV2, were confirmed as differentially expressed after 6 h of exposure. To the best of our knowledge, this is the first large-scale study reporting on potential gene expression modification associated with MMW radiation used in wireless communication applications.

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Section: Mmw At Intermediate Pd (5-15 Mw/cmmentioning

confidence: 99%

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Quément

Nicolaz

Desmots

et al. 2011

Bioelectromagnetics

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“…In fact this radiation is strongly absorbed by dipole-oriented molecules, such as water and phospholipids and a superficial-0.1-0.5 mm depth-heating of the tissue media can occur [16].…”

Section: Introductionmentioning

confidence: 99%

The influence of millimeter waves on the physical properties of large and giant unilamellar vesicles

et al. 2013

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Exposure of cell membranes to an electromagnetic field (EMF) in the millimeter wave band (30-300 GHz) can produce a variety of responses. Further, many of the vibrational modes in complex biomolecules fall in the 1-100 GHz range. In addition to fundamental scientific interest, this may have applications in the development of diagnostic and therapeutic medical applications. In the present work, lipid vesicles of different size were used to study the effects of exposure to radiation at 52-72 GHz, with incident power densities (IPD) of 0.0035-0.010 mW/cm 2 , on the chemical-physical properties of cell membranes. Large unilamellar vesicles (LUVs) were used to study the effect of the radiation on the physical stability of vesicles by dynamic light scattering. An inhibition of the aging processes (Ostwald ripening), which usually occur in these vesicles because of their thermodynamic instability, resulted. Giant unilamellar vesicles (GUVs) were used to study the effect of the radiation on membrane water permeability under osmotic stress by phase contrast microscopy. In this case, a decrease in the water membrane permeability of the irradiated samples was observed. We advance the hypothesis that both the above effects may be explained in terms of a change of the polarization states of water induced by the radiation, which causes a partial dehydration of the membrane and consequently a greater packing density (increased membrane rigidity). Electronic supplementary materialThe online version of this article

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“…The maximal power density at the cell level was equal to 0.14 mW/cm 2 . Additional data on the numerical and experimental MMW dosimetry for in vitro experiments are available in previous study (Zhadobov et al 2008). To monitor the temperature inside the MMW exposure system, we used a temperature data logger (HI141JH, HANNA Instruments, Lingolsheim, France) with two independent temperature sensors.…”

Section: Discussionmentioning

confidence: 99%

Absence of direct effect of low-power millimeter-wave radiation at 60.4 GHz on endoplasmic reticulum stress

et al. 2008

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Millimeter waves (MMW) at frequencies around 60 GHz will be used in the very near future in the emerging local wireless communication systems and the potential health hazards of artificially induced environmental exposures represent a major public concern. The main aim of this study was to investigate the potential effects of low-power MMW radiations on cellular physiology. To this end, the human glial cell line, U-251 MG, was exposed to 60.4 GHz radiation at a power density of 0.14 mW/cm(2) and potential effect of MMW radiations on endoplasmic reticulum (ER) stress was investigated. ER is very sensitive to environmental insults and its homeostasis is altered in various pathologies. Through several assay systems, we found that exposure to 60.4 GHz does not modify ER protein folding and secretion, nor induces XBP1 or ATF6 transcription factors maturation. Moreover, expression of ER-stress sensor, BiP/GRP78 was examined by real-time PCR, in exposed or non-exposed cells to MMW radiations. Our data demonstrated the absence of significant changes in mRNA levels for BiP/GRP78. Our results showed that ER homeostasis does not undergo any modification at molecular level after exposure to low-power MMW radiation at 60.4 GHz. This report is the first study of ER-stress induction by MMW radiations.

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Numerical and Experimental Millimeter-Wave Dosimetry for In Vitro Experiments

Cited by 36 publications

References 22 publications

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

The influence of millimeter waves on the physical properties of large and giant unilamellar vesicles

Absence of direct effect of low-power millimeter-wave radiation at 60.4 GHz on endoplasmic reticulum stress

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