Numerical calculation of light scattering from metal and dielectric randomly rough Gaussian surfaces using microfacet slope probability density function based method

Dual view transport of intensity phase microscopy is adopted to quantitatively study the regulation of adenosine triphosphate (ATP) on cellular mechanics. It extracts cell phases in real time from simultaneously captured under-and over-focus images. By computing the root-mean-square phase and correlation time, it is found that the cellular fluctuation amplitude and speed increased with ATP compared to those with ATP depletion. Besides, when adenylyl-imidodiphosphate (AMP-PNP) was introduced, it competed with ATP to bind to the ATP binding site, and the cellular fluctuation amplitude and speed decreased. The results prove that ATP is a factor in the regulation of cellular mechanics. To our best knowledge, it is the first time that the dual view transport of intensity phase microscopy was used for live cell phase imaging and analysis. Our work not only provides direct measurements on cellular fluctuations to study ATP regulation on cellular mechanics, but it also proves that our proposed dual view transport of intensity phase microscopy can be well used, especially in quantitative phase imaging of live cells in biological and medical applications.

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Degree of Polarization Calculation for Laser Backscattering from Typical Geometric Rough Surfaces at Long Distance

Shen

Zhang

Liu

et al. 2022

Remote Sensing

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Measurement of the degree of polarization of backscattering light from rough surfaces plays an important role in targets-detection applications. The polarization bidirectional reflectance function is the key to establish the relation between the polarization states of incidence and backscattering light. For the purpose of obtaining a polarized bidirectional reflectance distribution function (pBRDF) of a realistic, complicated target, it is decomposed as typical geometric surfaces and analytically calculated as the degree of polarization of the backscattering light, using a microfacet model, under conditions in which the scale of the target is far less than the target distance. In an experiment testing several typical geometric models, the results coincided with the theoretical calculation. The degree of polarization varied substantially as the rotation angle of the target changed, but showed little dependence on the size of target. The results have potential in applications discriminating between targets at different spatial orientations.

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Numerical calculation of light scattering from metal and dielectric randomly rough Gaussian surfaces using microfacet slope probability density function based method

Cited by 3 publications

References 59 publications

Deep learning for light scattering computation: Reconstructing light scattering fields from 1-D randomly rough surfaces as an example

Deep learning for light scattering computation: Reconstructing light scattering fields from 1-D randomly rough surfaces as an example

Measurements on ATP induced cellular fluctuations using real-time dual view transport of intensity phase microscopy

Degree of Polarization Calculation for Laser Backscattering from Typical Geometric Rough Surfaces at Long Distance

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