Numerical Chromosomal Abnormalities in Patients with Acute Lymphoblastic and Myeloid Leukemia in Iran

Abstract

The majority of cases of ALL demonstrate an abnormal karyotype, either in chro mosome nu mber or structural changes. Abnormal chro mosome number in childhood acute lymphoblastic leukemia defines distinct biological subgroups with a different response to treatment. The tubes are cultured with three different protocols to save time if one protocol failed. Cultures are then harvested, and cells are fixed and chromosome spreads are prepared. Of 25 patient studied, one patient had psudodiploid karyotype, three patients had tetra-ploid karyotype, four patients had low hypo-diploid karyotype, four patients had high hyper-diploid karyotype, five patients had low hyper-d iploid karyotype and eight patients had normal karyotype. Some other factors like, Age, Sex, Consanguinity, Hemoglob in, W BC count, and Type of the leukemia cell also have been evaluated. We found excess number of patients having hypodiploid karyotype but still response to treatment protocols were satisfactory. By co mparison between 4 different cultures methods, we find d irect method to be more efficient for ploidy analysis.

Efficacy of Immediate, 24 Hours Versus 48 Hours Culture Method in Cytogenetic Study of Pediatric Acute Lymphoblastic Leukemia

“…Malekasgar et al in their study on 25 pediatric patients which compared four different culture methods , found that direct method was more efficient for ploidy analysis [8]. According to Gordon et al chances of finding a chromosomally abnormal clone in their study were about the same with any of three methods that is direct, 24 hour and mitotic synchronisation methods [9].…”