This study investigates the influence of tooth layer orientation on the bending fatigue behaviour of Nylon 12 spur gears manufactured by a selective laser sintering process in an ‘on-edge’ configuration. The test gears were submitted to single tooth bending fatigue tests, and the thermal response of gears was recorded using Infrared thermography. The surface morphology of failed teeth was examined using microscopy. The effect of tooth layer orientation on the fatigue strength of selective laser sintering gears was evaluated by testing each tooth configuration. The tooth configurations with the highest and lowest strength were 40° and 160°, respectively. The performance of selective laser sintering gears was compared with that of injection moulded Nylon 66 gears. The injection moulded, selective laser sintering 40°, and selective laser sintering 160° gear teeth were tested at multiple loads to investigate the high cycle fatigue and low cycle fatigue behaviour. The bending fatigue life was highest for the injection moulded gears in both low cycle fatigue and high cycle fatigue regimes. The surface temperature was higher in selective laser sintering gears, with 160° configuration exhibiting the highest temperature. The difference between the temperatures of selective laser sintering 40° configuration and injection moulded gear magnified as the applied load increased. Consequently, the strength of the selective laser sintering 40° configuration with respect to injection moulded gear deteriorated in the low cycle fatigue region. Fractography indicated that crack propagation in selective laser sintering 40° and selective laser sintering 160° configurations were cross-laminar and inter-laminar, respectively. In the cross-laminar mode of propagation, the crack propagation was impeded by the layer structure. However, the crack propagation was expedited in the inter-laminar mode, as the crack path was along the interlayer region, which presents a path of least resistance.