Objective: To investigate the status of occupational well-being of medical staff in tuberculosis department and analyze its influencing factors, so as to provide a basis for improving the occupational well-being of medical staff in tuberculosis department. Methods: In May 2020, we adopted the method of cluster sampling to select staff members from the tuberculosis departments of the Affiliated Hospital of Hebei University and infectious disease hospital. A total of 139 medical staff were recruited as the research subjects, and were investigated using medical staff occupational well-being scale. Results: The total score of occupational well-being was 76.46±8.97 points, There were statistically significant differences (P<0.05) in occupational well-being score among tuberculosis medical staff with different age, years of work, job title, night shift, marital status, and occupational type. The in?uencing factors of occupational well-being were the night shift, years of work, occupational type, and marital status (P<0.05). Conclusion: The overall level of occupational well-being of tuberculosis medical staffs is moderate, and occupational well-being is affected by night shift, years of work, occupational type and marital status. It is recommended that managers take targeted measures to improve the occupational well-being of tuberculosis medical staff.