Nutrient management and medium reuse for cultivation of a cyanobacterial consortium at high pH and alkalinity

Paquette, Alexandre; Vadlamani, Agasteswar; Demirkaya, Cigdem; Strous, Marc; Siegler, Hector De la Hoz

doi:10.3389/fbioe.2022.942771

Cited by 6 publications

(5 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The carbon content of the biomass was calculated using the formula CH 1.81 N 0.17 O 0.20 P 0.013 S 0.009 (ref. 57 ) . The total protein content (Extended Data Fig.…”

Section: Methodsmentioning

confidence: 99%

An ATP-sensitive phosphoketolase regulates carbon fixation in cyanobacteria

Chang

Wang

et al. 2023

Nat Metab

View full text Add to dashboard Cite

Regulation of CO2 fixation in cyanobacteria is important both for the organism and global carbon balance. Here we show that phosphoketolase in Synechococcus elongatus PCC7942 (SeXPK) possesses a distinct ATP-sensing mechanism, where a drop in ATP level allows SeXPK to divert precursors of the RuBisCO substrate away from the Calvin–Benson–Bassham cycle. Deleting the SeXPK gene increased CO2 fixation particularly during light–dark transitions. In high-density cultures, the Δxpk strain showed a 60% increase in carbon fixation and unexpectedly resulted in sucrose secretion without any pathway engineering. Using cryo-EM analysis, we discovered that these functions were enabled by a unique allosteric regulatory site involving two subunits jointly binding two ATP, which constantly suppresses the activity of SeXPK until the ATP level drops. This magnesium-independent ATP allosteric site is present in many species across all three domains of life, where it may also play important regulatory functions.

show abstract

“…The carbon content of the biomass was calculated using the formula CH 1.81 N 0.17 O 0.20 P 0.013 S 0.009 (ref. 57 ) . The total protein content (Extended Data Fig.…”

Section: Methodsmentioning

confidence: 99%

An ATP-sensitive phosphoketolase regulates carbon fixation in cyanobacteria

Chang

Wang

et al. 2023

Nat Metab

View full text Add to dashboard Cite

show abstract

“…In previous studies, the pH increase was attributed to a reduction in bicarbonates in the form of CO 2 fixation in the cultures, enabled by photosynthesis [46,47]. This invariably leads to a reduction in total alkalinity concentration [46][47][48][49], which serves as a source of inorganic carbon for growth. A similar occurrence was also observed in this experiment.…”

Section: Operational and Environmental Conditionsmentioning

confidence: 94%

Exploration of Microalgae-Activated Sludge Growth Performance in Lab-Scale Photobioreactors under Outdoor Environmental Conditions for Wastewater Biotreatment

James,

Bankole,

Pompei

et al. 2023

Phycology

View full text Add to dashboard Cite

Increasing the volume of untreated and inadequately treated municipal wastewater undermines the circular economy potential of wastewater resources, particularly in low-income regions. This present study focused on and evaluated the performance of native microalgae-activated sludge (MAS) growth for tertiary treatment of anaerobically digested wastewater from an up-flow anaerobic sludge blanket (UASB) in an outdoor lab-scale photobioreactor (2.2 L). Three conditions with distinct MAS inoculum concentrations alongside three controls were operated in batch mode for 5 days hydraulic retention time (HRT) at 11.5:12.5 photo-hours. The MAS inoculum concentration influenced the treatment outcome. The best performance was observed when the MAS concentration was 0.10/0.20 g L−1, and the cell density was 1.60 × 107 cells mL−1, total biomass productivity of 0.10 g TSS L−1 d−1, total phosphorus uptake of 85.1%, and total nitrogen uptake of 66.1%. Logarithmic removal (Log-Re) of bacterial pathogens (water quality indicators) showed Log-Re 3.4 for total coliforms (1.37 × 102 CFU 100 mL−1) and 4.7 for Escherichia coli (0.00 × 100 CFU 100 mL−1). The results revealed optimum remediation performance and nutrient recovery potential with appropriate inoculum concentration, in admiration to advancing the science of circular economy.

show abstract

“…Pore water concentrations of sodium (Na), calcium (Ca), magnesium (Mg), potassium (K), and iron (Fe) were measured using Inductively Coupled Plasma Mass Spectrometry (ICP-QQQ, Agilent Technologies, Tokyo, Japan) [47]. Brie y, pore water samples were diluted 400x with 5% nitric acid to reach a pH of ~ 3.0 and then ltered using 0.2 µm Acrodisc® PSF Syringe Filters (Pall Corporation, East Hills, NY).…”

Section: Anions and Cationsmentioning

confidence: 99%

“…Calibration curves were then used to determine the concentration of each element in the sediment samples. Concentrations of sulfate, phosphate, nitrate, and chloride were measured using an ion chromatography system equipped with an IonPac AS18 anion-exchange column and a conductivity detector (DIONEX ICS-5000+, Thermo Fisher Scienti c, USA) [47,51]. Pore water samples were diluted 100x using Mili-Q water and ltered using 0.2 µm mixed cellulose esters (MCE) membrane lters.…”

Section: Anions and Cationsmentioning

confidence: 99%

“…Frozen sediment was then transferred to a benchtop freeze dryer, lyophilized at -50°C, and at a pressure of 1 mPa (Labconco, Kansas City, MO). After 72 hours, the freeze-dried biomass was removed, and the carbon, nitrogen, and hydrogen content was determined using a CHN Elemental Analyzer (Perkin Elmer 2400 Series II CHNS/O, Massachusetts, USA) [47].…”

Section: Chn Analysis Pb-210 Dating and Xrfmentioning

confidence: 99%

See 1 more Smart Citation

Ecology and biogeochemistry of the microbial underworld in two sister soda lakes

Paquette,

Bhatnagar,

Vadlamani

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Approximately 3.7 billion years ago, microbial life may have emerged in phosphate-rich salty ponds. Surprisingly, analogs of these environments are present in alkaline lake systems, recognized as highly productive biological ecosystems. Investigating the microbial ecology of two Canadian soda lake sediment systems characterized by naturally high phosphate levels. Using a comprehensive approach involving geochemistry, metagenomics, and amplicon sequencing, we discovered that groundwater infiltration into Lake Goodenough sediments supported stratified layers of microbial metabolisms fueled by decaying mats. Effective degradation of microbial mats resulted in unexpectedly low net productivity. Evaporation of water from Last Chance Lake and its sediments led to saturation of brines and a habitat dominated by inorganic precipitation reactions, with low productivity, low organic matter turnover and little biological uptake of phosphorus, leading to high phosphate concentrations. Our research highlights that modern analogs for origin-of-life conditions might be better represented by soda lakes with low phosphate concentrations. Highly alkaline brines were found to be dominated by potentially dormant spore-forming bacteria. These saturated brines also hosted potential symbioses between Halobacteria and Nanoarchaeaota, as well as Lokiarchaea and bacterial sulfate reducers. Metagenome-assembled genomes of Nanoarchaeaota lacked strategies for coping with salty brines and were minimal for Lokiarchaea. Thus, highly alkaline brine environments could be too extreme to support origin of life scenarios. These findings shed light on the complex interplay of microbial life in extreme environments and contribute to our understanding of early Earth environments.

show abstract

Nutrient management and medium reuse for cultivation of a cyanobacterial consortium at high pH and alkalinity

Cited by 6 publications

References 72 publications

An ATP-sensitive phosphoketolase regulates carbon fixation in cyanobacteria

An ATP-sensitive phosphoketolase regulates carbon fixation in cyanobacteria

Exploration of Microalgae-Activated Sludge Growth Performance in Lab-Scale Photobioreactors under Outdoor Environmental Conditions for Wastewater Biotreatment

Ecology and biogeochemistry of the microbial underworld in two sister soda lakes

Contact Info

Product

Resources

About