Nutrient Removal from Mainstream Anaerobic Processes using a Membrane Biofilm Reactor and a Granular Sludge Sequencing Batch Reactor

Delgado-Vela, Jeseth; Martin, Kelly J.; Beaton, Nigel; McFarland, Andrea; Stadler, Lauren B.; Bott, Charles; Skerlos, Steven J.; Raskin, Lutgarde; Love, Nancy G.

doi:10.2175/193864715819539795

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“…Details of reactor startup conditions and inocula are provided in the Supporting Information Section 1. Briefly, the reactor was inoculated with a mixture of a previously established community (Delgado Vela et al ., 2015a) and activated sludge from an Anaerobic–Anoxic–Aerobic activated sludge process (Ann Arbor, MI).…”

Section: Methodsmentioning

confidence: 99%

Sulfide alters microbial functional potential in a methane and nitrogen cycling biofilm reactor

Vela

Bristow

Marchant

et al. 2020

Environmental Microbiology

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Cross-feeding of metabolites between coexisting cells leads to complex and interconnected elemental cycling and microbial interactions. These relationships influence overall community function and can be altered by changes in substrate availability. Here, we used isotopic rate measurements and metagenomic sequencing to study how cross-feeding relationships changed in response to stepwise increases of sulfide concentrations in a membraneaerated biofilm reactor that was fed with methane and ammonium. Results showed that sulfide: (i) decreased nitrite oxidation rates but increased ammonia oxidation rates; (ii) changed the denitrifying community and increased nitrous oxide production; and (iii) induced dissimilatory nitrite reduction to ammonium (DNRA). We infer that inhibition of nitrite oxidation resulted in higher nitrite availability for DNRA, anammox, and nitrite-dependent anaerobic methane oxidation. In other words, sulfide likely disrupted microbial cross-feeding between AOB and NOB and induced cross-feeding between AOB and nitrite reducing organisms. Furthermore, these cross-feeding relationships were spatially distributed between biofilm and planktonic phases of the reactor. These results indicate that using sulfide as an electron donor will promote N 2 O and ammonium production, which is generally not desirable in engineered systems.

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Section: Methodsmentioning

confidence: 99%