Nutrient Starvation Exposure Induced the Down-Regulation of Stress Responsive Genes and Selected Bioactive Metabolic Pathways in Phaeodactylum tricornutum

Abstract

The microalga Phaeodactylum tricornutum is considered a model diatom. It is the second diatom whose genome was sequenced and the first one genetically engineered. This permits its use as a cell factory for the production of high-value compounds for nutraceutical, cosmeceutical, pharmaceutical, biodiesel, and bioplastic applications. This study is focused on analyzing expression levels of enzymes involved in the synthesis of sulfoglycolipids and monogalactosyldiacylglycerols, compounds known to have anticancer and immunomodulatory activities, and genes coding antioxidant, heat shock and stress-responsive proteins, in various culturing conditions. Our data showed that both nutrient starvation and senescence induced the down-regulation of both sulfoglycolipid and monogalactosyldiacylglycerol synthesis-related genes and stress-responsive genes (compared to the replete condition), suggesting that the control condition, consisting of cells in the exponential phase in replete medium, is the condition with the highest expression of the genes of interest and worth of further bioactivity screening and chemical analyses for drug discovery and biotechnological applications.