Nutritional impact on gene expression and competence of oocytes used to support embryo development and livebirth by cloning procedures in goats

Fernandes, César Carneiro Linhares; Aguiar, L. H.; Calderón, C. E. M.; Silva, A.M.; Alves, Juliana Paula Martins; Rossetto, R.; Bertolini, Luciana Relly; Bertolini, Marcelo; Rondina, D.

doi:10.1016/j.anireprosci.2017.10.012

Cited by 9 publications

(7 citation statements)

References 66 publications

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“…Thus, the exponential proliferation of granulosa cells from gonadotrophin‐mediated hormone stimulation for antrum acquisition and improvements in oocyte competence becomes essential (Menchaca, Barrera, Santos‐Neto, Cuadro, & Crispo, ). Therefore, regardless of the type of treatment used, oocyte recovery rates observed in this study (72%) are consistent with the data obtained by other researchers (50%–91%) (Fernandes et al, ; Menchaca et al, ; Sanchez‐Lazo et al, ).…”

Section: Discussionsupporting

confidence: 93%

“…This mechanism leads to an increase in circulating concentrations of non‐esterified fatty acids (NEFA) during the end of gestation and the post‐partum period (Magistrelli & Rosi, ). Recent researches have shown that the use of fat reserves in goats is influenced by energy diets, with limitations in lipolysis (Invernizzi et al, ) and increase in sternal subcutaneous fat thickness (Fernandes et al, ), with maximum values of 11.4, which are similar to that found in the present study (10.5).…”

Section: Discussionsupporting

confidence: 89%

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Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

Alves

Fernandes

Rossetto

et al. 2019

Reprod Domestic Animals

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The aim of this study was to verify the effect of the energy source for a short‐term diet supplementation on follicular dynamics, ovarian response and oocyte recovery in goats. Thirty Anglo Nubian crossbred does received a diet for 4 weeks to satisfy the nutritional requirements of breeding for adult non‐dairy goats. Seven days prior to oocyte recovery (OR), a group of does (n = 10) was supplemented with ground full‐fat linseed in the diet (Diet A), whereas a second group of does (n = 10) received crude glycerine in the diet (Diet B). The total mixed ration (TMR) diet was maintained as the Control Diet (n = 10). All animals were oestrous‐synchronized by the use of a progesterone insert for 12 days prior to OR. Follicles were stimulated by using pFSH (five 40‐mg/ml doses) during the supplementation time. At OR, follicles were counted and recovered oocytes were classified as viable or degenerated. Follicular dynamics was monitored by ultrasonography, and plasma glucose, cholesterol and triglyceride levels were measured during supplementation. Glucose was higher in Diet B and cholesterol in Diet A. Diet B had a lower proportion of small (<3 mm) and large follicles (≥3 mm; p = 0.01). The follicular growth rate was higher in Diet A (p < 0.01), with follicles emerging in the 5th day of supplementation. No differences were observed for follicles counted and oocytes recovered. Thus, the type of energy source supplemented for a short term was capable to alter the follicular dynamics, without affecting the proportion of morphologically viable oocytes upon recovery.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 89%

Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

Alves

Fernandes

Rossetto

et al. 2019

Reprod Domestic Animals

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“…Thus, the use of high levels of wheat and corn glutens is another important aspect of the present study. Some active substances found in feedstuff are known to either induce or inhibit the secretion of ovarian hormones [20][21][22][23][24][25]. It has been reported that, in the event of a negative energy balance, plasma insulin and glucose levels decrease, GnRH secretion and LH release are reduced, and in result, both follicular development and estrogenic activities are adversely affected and ovulation is prolonged [20,22].…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the histopathological analyses performed in the ovarian tissue samples of the animals at the end of the trial showed that primordial follicle, primary follicle, secondary follicle, graafian follicle, atrophic follicle and, corpora lutea parameters were no statistically significant difference between the study groups (Table 3). Although literature reports are available on the effects of nutrients on fertility [21][22][23][24][25], to the authors' knowledge, there is no previous study specific to the effects of glutens on follicular development. Therefore, a comparison of the findings obtained in the present study was not able to be made.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Dietary Wheat and Corn Glutens on the Histopathological and Immunohistochemical Structure of the Ovarian Tissue and Serum and Ovarian Tissue LH and FSH Levels and Lipid Profiles in Rats

İmik

Kapakin

Karabulutlu

et al. 2023

Braz. arch. biol. technol.

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This study was aimed at determining the effects of corn and wheat glutens on the histopathological and immunohistochemical structure of the ovarian tissue, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels, and lipid profile in rats. Twenty-day-old 24 female Sprague-Dawley rats were assigned to three groups, and were raised until 185 days of age. Three study groups, named as Wheat, Corn and Soybean Groups, were established and fed on wheat gluten, corn gluten and soybean meal, respectively, as a protein source. At the end of the trial, ovarian tissue specimens and serum samples were taken from the animals, and analyzed. Compared to Soybean Group, in Wheat Group, of the ovarian histopathological parameters investigated, values pertaining to the primordial, primary, secondary, and Graafian follicles and corpora lutea (CL) were numerically smaller, and for the immunohistochemical parameters investigated, in the transglutaminase 2 (TGM2), gliadin, IgA, IgM, CD4 and CD8 were immunopositivity higher (P>0.05). It was determined that, in Wheat Group, ovarian tissue LH levels had significantly decreased, whilst serum HIGHLIGHTS• The cereals are a significant food source for both humans and animals.• The balanced nutrition is critical to the development of the reproductive system.• The serum lipid profile is critical to the development of multiple diseases.• Dietary gluten has effects on the immunohistochemical structure of the ovarian tissue.

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“…The in vitro maturation of oocytes was considered to be an important technical link of embryo engineering technology, which aims to further explore the potential of animal reproduction and provide effective solutions for large numbers of breeding high‐quality livestock, such as cattle (Zoheir et al., 2017) and goats (Ren et al., 2018; Yin et al., 2017). In vitro production (IVP) can improve the original genetic characteristics and reproductive capacity of oocytes through artificial factors, which has great potential for the research of improving livestock, such as goats (Fernandes et al., 2018). Although some achievements have been made in goat oocyte maturation in vitro and embryo production technology in vitro, oocytes matured in vivo have much better quality and post‐fertilization than those in vitro (Thomas et al., 2004 ) , which should that there were still many problems with the in vitro maturation (IVM) of oocytes (Saeedabadi et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

Alpha‐lipoic acid improves the maturation and the developmental potential of goat oocytes in vitro

Wang

Zhang

et al. 2021

Reprod Domestic Animals

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Oxidative stress inevitably occurs during oocyte maturation in vitro. α‐lipoic acid (α‐LA) has a strong antioxidant capacity, but the effect of α‐LA on parthenogenetic activation of oocytes was rarely reported. This study aims to investigate the effect of supplementing α‐LA to in vitro maturation medium on the subsequent developmental ability of goat parthenogenetic embryos during oocytes maturation. In the study, the goat cumulus‐oocyte complex was divided into the experimental (with 25 μmol/L α‐LA) and the control (without α‐LA) groups. Oxidase expression was measured using RT‐qPCR. After 18–22 hr of maturation, the oocytes were then parthenogenetic activated. The total antioxidant capacity of embryos was measured after 0, 24, 48, 72 and 96 hr of culture. Rates of oocyte maturation and the rates of development for parthenogenetic embryos in the α‐LA group were significantly improved by 7.88% (p < .05) and 5.41% (p < .05) compared with those in the control group, respectively. After 24 hr, the difference in total antioxidant capacity was extremely significant in both groups. An evident decrease in the control group and a minor decrease in the α‐LA group were observed (p < .01). The ratio of inner cell mass cells to the total cell number of blastocysts in the α‐LA group increased compared with that in the control group (p < .05) on day 8. α‐LA significantly promoted the expression of SOD and GPX4 of parthenogenetic blastocysts and maturated oocytes. α‐LA (25 μmol/L) improved the maturation rate and the developmental competence of the parthenogenetic activation of oocytes, which might be mediated by maintaining the total antioxidant ability of oocytes during the culture period.

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Nutritional impact on gene expression and competence of oocytes used to support embryo development and livebirth by cloning procedures in goats

Cited by 9 publications

References 66 publications

Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

The Effects of Dietary Wheat and Corn Glutens on the Histopathological and Immunohistochemical Structure of the Ovarian Tissue and Serum and Ovarian Tissue LH and FSH Levels and Lipid Profiles in Rats

Alpha‐lipoic acid improves the maturation and the developmental potential of goat oocytes in vitro

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