The results of field researches on the experimental fields of the Agrobiotechnopark of KSAU conducted in 2020-2022 are given. The aim of the research was to evaluate the effect of double treatment during the growing season with various biological preparations on the formation of the crop and the quality of spring barley grain. The objectives of the research included studying the nature of changes in the biometric parameters of plants, leaf surface area, yield and protein content in grain when using a biofungicide and biofertilizer. The variety of spring two-row barley Kamashevsky was the object of research. Biopreparations were studied - biofungicide Orgamica S (biological agent - Bacillus amyloliquefaciens) and biofertilizer Organit P (biological agent - Bacillus megaterium). The treatment was carried out twice during the growing season - in the tillering phase and in the earing phase with a working fluid flow rate of 200 l/ha. The preparations were used separately and as part of a tank mixture. The studies were carried out on a gray forest highly cultivated soil. The agro-climatic conditions of vegetation during the years of the study varied significantly. In 2021, soil-air drought was noted, and in 2020, and especially in 2022, the moisture conditions were favorable for the growth and development of spring barley plants. It has been established that the treatment of spring barley crops with biological preparations contributes to an increase in the length of the ear, stems and roots. This effect was especially significant when using a biofertilizer in the tillering phase, and a biofungicide during the heading of barley. The use of all treatment schemes with biological preparations showed an increase in the area of spring barley leaves and, at the same time, the development of root rots of the crop decreased by an average of 50%. The greatest increase in yield (by 0.56 t/ha), the maximum protein content in the grain and the best profitability indicators were obtained using a treatment scheme in which Organit biofertilizer was used during the tillering period, and Orgamica S biofungicide during the heading period. Especially significant positive effect from treatment with biological preparations was in the conditions of a well-moistened 2022, but even in the conditions of a drought in 2021, treatment with biological preparations showed high efficiency.