Nylon mesh-based 3D scaffolds for the adherent culture of neural stem/progenitor cells

Mori, Hideki; Naka, Ryosuke; Fujita, Masanori; Hara, Masayuki

doi:10.1016/j.jbiosc.2020.12.003

Cited by 3 publications

(3 citation statements)

References 54 publications

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“…Fiber-diameter dependent effects might be derived from different interaction mechanisms between cells and scaffold. Rodent neurons cultured on aligned ultrathin nanofibers (60 nm) expressed β1-integrin focal adhesions throughout the entire cell surface (cell soma and neurites), while those growing on aligned submicron-fibres (300 nm) formed these structures only on the cell soma ( Mori et al, 2021 ). Czeisler et al showed how fibres ( Figure 5C ) with dimensions similar to radial glia (Ø = 1 μm) and small blood vessels (Ø = 10 μm), mimicking anatomical features that neural cells encounter during development, resulted in differential responses from rodent neural stem cells.…”

Section: Applications Of Engineered Microenvironments In Neuromechano...mentioning

confidence: 99%

Engineered cell culture microenvironments for mechanobiology studies of brain neural cells

Ransanz

Altena²,

Heine³

et al. 2022

Front. Bioeng. Biotechnol.

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The biomechanical properties of the brain microenvironment, which is composed of different neural cell types, the extracellular matrix, and blood vessels, are critical for normal brain development and neural functioning. Stiffness, viscoelasticity and spatial organization of brain tissue modulate proliferation, migration, differentiation, and cell function. However, the mechanical aspects of the neural microenvironment are largely ignored in current cell culture systems. Considering the high promises of human induced pluripotent stem cell- (iPSC-) based models for disease modelling and new treatment development, and in light of the physiological relevance of neuromechanobiological features, applications of in vitro engineered neuronal microenvironments should be explored thoroughly to develop more representative in vitro brain models. In this context, recently developed biomaterials in combination with micro- and nanofabrication techniques 1) allow investigating how mechanical properties affect neural cell development and functioning; 2) enable optimal cell microenvironment engineering strategies to advance neural cell models; and 3) provide a quantitative tool to assess changes in the neuromechanobiological properties of the brain microenvironment induced by pathology. In this review, we discuss the biological and engineering aspects involved in studying neuromechanobiology within scaffold-free and scaffold-based 2D and 3D iPSC-based brain models and approaches employing primary lineages (neural/glial), cell lines and other stem cells. Finally, we discuss future experimental directions of engineered microenvironments in neuroscience.

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Section: Applications Of Engineered Microenvironments In Neuromechano...mentioning

confidence: 99%

Engineered cell culture microenvironments for mechanobiology studies of brain neural cells

Ransanz

Altena²,

Heine³

et al. 2022

Front. Bioeng. Biotechnol.

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show abstract

“…3G) (see Scheme 8 in reference [42]). For example, a polyacrylic acid chain was synthesized on the surface of polyamide (nylon) fiber [65]. This technique can be applied to both synthetic polymers and biopolymers.…”

Section: Selectivity Of Amino Acids In Reaction With Radicalsmentioning

confidence: 99%

Effects of Ionizing Radiation on Biopolymers for Applications as Biomaterials

Hara¹

2022

Biomedical Materials & Devices

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Biopolymers, including extracellular matrix proteins and polysaccharides, are used as substrates for cell culture and scaffolds in tissue engineering. Ionizing radiation, for example with gamma rays or electron beams, is used to sterilize materials and to modify the physical and chemical properties of materials. The principles of the effect of ionizing radiation on biopolymers are discussed in this review article. Among the types of ionizing radiation, gamma rays and electron beams are widely used to sterilize biomaterials and construct medical devices. Water molecules are split by irradiation with ionizing radiation to give hydrated electrons, proton radicals and hydroxyl radicals. These reactive molecular species react with polymer chains to provoke scission or crosslinking reactions. Both the scission and crosslinking of the polymer chains can occur, depending on the types of polymer and irradiation conditions. Sterilization of materials is necessary if they are used in medical devices and implants. The advantages and disadvantages of ionizing radiation in comparison with other methods of sterilization are also discussed. Irradiations with strong gamma rays on the three types of biopolymers-Type I collagen, wool keratin and alginate-are described as examples of biopolymers. Ionizing radiation is a very useful tool for sterilizing materials and constructing medical devices, but it is important that we know its merits and demerits, as well as the limitations, safety and risks, of its applications.

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“…Previous studies have considered the induction behavior of cells modulated by the parameters of 3D scaffolds such as pore size [ 12 , 13 , 14 , 15 ], pore shape [ 16 , 17 , 18 ], surface roughness [ 19 , 20 , 21 ], support strength [ 22 , 23 ], and wettability [ 21 ]. A large number of studies have focused on the influence of the porosity and pore size of the 3D microscaffold on cell behaviors.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Cell Behavior by 3D Biocompatible Hydrogel Microscaffolds with Precise Configuration

et al. 2021

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Three-dimensional (3D) micronano structures have attracted much attention in tissue engineering since they can better simulate the microenvironment in vivo. Two-photon polymerization (TPP) technique provides a powerful tool for printing arbitrary 3D structures with high precision. Here, the desired 3D biocompatible hydrogel microscaffolds (3D microscaffold) with structure design referring to fibroblasts L929 have been fabricated by TPP technology, particularly considering the relative size of cell seed (cell suspension), spread cell, strut and strut spacing of scaffold. Modulation of the cell behavior has been studied by adjusting the porosity from 69.7% to 89.3%. The cell culture experiment results reveal that the obvious modulation of F-actin can be achieved by using the 3D microscaffold. Moreover, cells on 3D microscaffolds exhibit more lamellipodia than those on 2D substrates, and thus resulting in a more complicated 3D shape of single cell and increased cell surface. 3D distribution can be also achieved by employing the designed 3D microscaffold, which would effectively improve the efficiency of information exchange and material transfer. The proposed protocol enables us to better understand the cell behavior in vivo, which would provide high prospects for the further application in tissue engineering.

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Nylon mesh-based 3D scaffolds for the adherent culture of neural stem/progenitor cells

Cited by 3 publications

References 54 publications

Engineered cell culture microenvironments for mechanobiology studies of brain neural cells

Engineered cell culture microenvironments for mechanobiology studies of brain neural cells

Effects of Ionizing Radiation on Biopolymers for Applications as Biomaterials

Modulation of Cell Behavior by 3D Biocompatible Hydrogel Microscaffolds with Precise Configuration

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