O-Antigen Serotypes and Type III Secretory Toxins in Clinical Isolates of
            <i>Pseudomonas aeruginosa</i>

Faure, Karine; Shimabukuro, David; Ajayi, Temitayo; Allmond, Leonard R.; Sawa, Teiji; Wiener-Kronish, Jeanine P.

doi:10.1128/jcm.41.5.2158-2160.2003

Cited by 46 publications

(49 citation statements)

References 37 publications

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“…exoS was more frequent in isolates from urinary tract and wound infections from non-ICU patients, a finding that is in accordance with those by other investigators (18). exoU was associated with serotype O11, as reported also by Faure et al (4), and detected mainly among ICU isolates (P ϭ 0.032). Expression of exoU correlates with acute cytotoxicity and accelerated lung injury playing a role in the development of septic shock in ICU high-risk patients (18,19).…”

supporting

confidence: 82%

“…The large number of secreted and cell-associated virulence factors is implicated in the pathogenesis of severe infections. The type III secretion system (TTSS), a complex of three proteins, is associated with lung injury, sepsis, and a 6-fold-greater risk of mortality, constituting an important virulence determinant (4,5). Results on emergence and spread of MDRPA isolates in the University Hospital of Patras (UHP) and their phenotypic and genotypic characteristics are presented in this study.…”

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confidence: 99%

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Spread of Multidrug-Resistant Pseudomonas aeruginosa Clones in a University Hospital

et al. 2013

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An outbreak of multidrug-resistant Pseudomonas aeruginosa (MDRPA) infections in a university hospital is described. Phenotypic and genotypic analysis of 240 isolates revealed that 152 patients, mainly in the intensive care unit (ICU), were colonized or infected with MDRPA, the majority with O11. All metallo-␤-lactamase (MBL)-positive isolates carried the bla VIM-2 or bla VIM-1 gene. One or more type III secretion system toxin genes were detected in most isolates. Five dominant pulsed-field gel electrophoresis (PFGE) types were characterized, associated with ST235, ST111, ST253, ST309, and ST639.

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confidence: 82%

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confidence: 99%

Spread of Multidrug-Resistant Pseudomonas aeruginosa Clones in a University Hospital

et al. 2013

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“…With this in mind, we searched the published genome of 2192 (13), and we recognized the serotype O1 O-antigen locus. Serotype O1 is a clinically relevant serotype (14), and its O-antigen subunit structure is N-acetylgalactosamine, 2-acetamido-3-amino-2, 3…”

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confidence: 99%

Identification of the Mutation Responsible for the Temperature-Sensitive Lipopolysaccharide O-Antigen Defect in the Pseudomonas aeruginosa Cystic Fibrosis Isolate 2192

et al. 2013

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cPseudomonas aeruginosa in the lungs of cystic fibrosis (CF) patients is characterized by a series of genotypic and phenotypic changes that reflect the transition from acute to chronic infection. These include the overproduction of the exopolysaccharide alginate and the loss of complete lipopolysaccharide (LPS). LPS is a major component of the Gram-negative outer membrane and is composed of lipid A, core oligosaccharide, and O antigen. In this report, we show that the LPS defect of the P. aeruginosa chronic infection isolate 2192 is temperature sensitive. When grown at 25°C, 2192 expresses serotype O1 LPS with a moderate chain length and in reduced amounts relative to those of a wild-type serotype O1 laboratory strain (stO1). In contrast, 2192 expresses no LPS O antigen when grown at 37°C. This is the first time that a temperature-sensitive defect in O-antigen production has been reported. Using complementation analyses with a constructed wbpM deletion mutant of stO1, we demonstrate that the temperature-sensitive O-antigen production defect in 2192 is due to a mutation in wbpM, which encodes a UDP-4,6-GlcNAc dehydratase involved in O-antigen synthesis. The mutation, a deletion of a single amino acid (V636) from the extreme C terminus of WbpM, renders the protein less stable than its wild-type counterpart. This residue of WbpM, which is critical for stability and function, is located outside of the recognized domains of the protein and may provide insight into the structure-function relationship of this enzyme, which is found in all 20 serotypes of P. aeruginosa. We also identify a promoter of wbpM, map a transcriptional start site of wbpM, and show that mucoidy plays a role in the loss of expression of high-molecular-weight LPS in this CF isolate.

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confidence: 99%

“…This serogroup is one of the most prevalent (1) and has been associated with acute infections (2), burns (3), and ulcerative keratitis (4). In addition, P. aeruginosa serogroup O11 strains are among the most pathogenic and lethal because of the expression of the cytotoxic phospholipase ExoU (2). When the P. aeruginosa serogroup O11 O antigen is expressed in Escherichia coli from the cosmid pLPS2, it is attached to the E. coli lipid A-core (5); when Salmonella contains pLPS2, P. aeruginosa serogroup O11 expression does not exclude expression of Salmonella O antigen (6).…”

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confidence: 99%

Intranasal immunization with heterologously expressed polysaccharide protects against multiple Pseudomonas aeruginosa infections

DiGiandomenico

Rao

Harcher

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Surface-expressed bacterial polysaccharides are often immunodominant, protective antigens. However, these antigens are chemically and serologically highly heterogeneous, and conjugation to protein carriers is often necessary to enhance their immunogenicity. Here we show the efficacy of intranasal immunization of mice with attenuated Salmonella enterica serovar Typhimurium expressing the O antigen portion of Pseudomonas aeruginosa lipopolysaccharide. P. aeruginosa is an ideal model system because it can cause a myriad of localized and systemic infections. In particular, this bacterium is a leading cause of hospital-acquired pneumonia and is responsible for infections after burns and after eye injury. In addition, there are mouse models of infection that mimic the clinical manifestations of P. aeruginosa infections. Immunized mice were highly protected against infection, with long-lasting immunity to acute P. aeruginosa pneumonia, whereas mice immunized with Salmonella containing only the cloning vector or PBS were not. Prophylactic and therapeutic administration of sera from vaccinated animals protected naive mice. Intranasal vaccination also provided complete protection from infections after burns and reduced pathology after corneal abrasions. These results indicate that intranasal delivery of heterologously expressed polysaccharide antigens provides protection at distinct sites of infection. This approach for the expression and delivery of polysaccharide antigens as recombinant immunogens could be easily adapted to develop vaccines for many infectious agents, without the need for complicated purification and conjugation procedures.antibody ͉ Salmonella ͉ vaccine B acterial surface polysaccharides make effective components for vaccines against serious infections because they are generally immunodominant. However, their utility is limited by significant chemical, and hence serologic, variability that requires a large number of components for comprehensive coverage against pathogens capable of expressing different polysaccharide serotypes. Also, purified polysaccharides are often poorly immunogenic, necessitating synthesis of protein-polysaccharide conjugate vaccines. These problems could potentially be overcome by use of recombinant DNA to induce synthesis of the polysaccharides in a heterologous antigen-delivery system. We evaluated this potential by expressing the Pseudomonas aeruginosa LPS O antigen in attenuated Salmonella enterica serovar Typhimurium SL3261 and used this construct to immunize mice intranasally (IN) to evaluate protective immunity against P. aeruginosa infection in the lung and eye, as well as systemic infection emanating from burn wounds.The model polysaccharide that we have used for this study is the P. aeruginosa serogroup O11 O antigen. This serogroup is one of the most prevalent (1) and has been associated with acute infections (2), burns (3), and ulcerative keratitis (4). In addition, P. aeruginosa serogroup O11 strains are among the most pathogenic and lethal because of the expression ...

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O-Antigen Serotypes and Type III Secretory Toxins in Clinical Isolates of Pseudomonas aeruginosa

Cited by 46 publications

References 37 publications

Spread of Multidrug-Resistant Pseudomonas aeruginosa Clones in a University Hospital

Spread of Multidrug-Resistant Pseudomonas aeruginosa Clones in a University Hospital

Identification of the Mutation Responsible for the Temperature-Sensitive Lipopolysaccharide O-Antigen Defect in the Pseudomonas aeruginosa Cystic Fibrosis Isolate 2192

Intranasal immunization with heterologously expressed polysaccharide protects against multiple Pseudomonas aeruginosa infections

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