Oat Peptides Alleviate Dextran Sulfate Sodium Salt-Induced Colitis by Maintaining the Intestinal Barrier and Modulating the Keap1-Nrf2 Axis

Abstract

The prevalence of inflammatory bowel disease (IBD) is progressively rising each year, emphasizing the significance of implementing rational dietary interventions for disease prevention. Oats, being a staple agricultural product, are abundant in protein content. This study aimed to investigate the protective effects and underlying mechanisms of oat peptides (OPs) in a mouse model of acute colitis induced by dextran sulfate sodium salt (DSS) and a Caco-2 cell model. The findings demonstrated that intervention with OPs effectively mitigated the symptoms associated with DSS-induced colitis. The physicochemical characterization analysis demonstrated that the molecular weight of the OPs was predominantly below 5 kDa, with a predominant composition of 266 peptides. This study provides further evidence of the regulatory impact of OPs on the Keap1-Nrf2 signaling axis and elucidates the potential role of WGVGVRAERDA as the primary bioactive peptide responsible for the functional effects of OPs. Ultimately, the results of this investigation demonstrate that OPs effectively mitigate DSS-induced colitis by preserving the integrity of the intestinal barrier and modulating the Keap1-Nrf2 axis. Consequently, these findings establish a theoretical foundation for the utilization of OPs as dietary supplements to prevent the onset of IBD.

Wheat peptide alleviates DSS-induced colitis by activating the Keap1–Nrf2 signaling pathway and maintaining the integrity of the gut barrier

“…The number of goblet cells was measured using ImageJ software. The pathological evaluation was performed according to previous methods, 20 and the histological score was based on mucosal edema (0–3), crypt destruction and loss (0–4), inflammatory cell infiltration (0–3), and epithelial cell disruption (0–3).…”

Oyster Peptides Ameliorate Dextran Sulfate Sodium-Induced Ulcerative Colitis via Modulating the Gut Microbiota and Inhibiting the TLR4/NF-κB Pathway

“…The correlation analysis methods used here refer to our previous studies [30][31][32]. Briefly, the correlations of DAI scores, body weight changes, differential bacteriophages, inflammation, and oxidative stress metrics in mice were analyzed and network plotted using OmicStudio tools (https://www.omicstudio.cn/tool, accessed on 16 October 2023).…”