OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese

Kang, Bo; Jiang, Dongmei; Ma, Rong; He, Hua; Zhang, Yi; Chen, Ziyu

doi:10.1371/journal.pone.0175016

Cited by 11 publications

(11 citation statements)

References 42 publications

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“…Polyamine contents were measured by HPLC using an Agilent 1100 Series system (Agilent Technologies, CA, USA) following a benzoylation procedure as described previously [ 18 ]. A 20 µL of benzoyl polyamines were separated on a 5 μm particle size C18, 4.6 × 250 mm column (Agilent Technologies).…”

Section: Methodsmentioning

confidence: 99%

Exogenous spermidine affects polyamine metabolism in the mouse hypothalamus

Jiang

et al. 2021

Open Life Sciences

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Spermidine is important for the hypothalamic control of pituitary secretion of hormones involved in neuroendocrine functions in mammals. In this study, the effect of exogenous spermidine on the expression of genes and proteins related to polyamine metabolism and polyamine levels was examined. The results indicated that treatment with spermidine at 0.05 mg/g (BW) significantly increased the levels of Oaz1 mRNA and protein expression and decreased putrescine content in mouse hypothalamus (p < 0.05). The administration with spermidine at 0.10 mg/g significantly increased the levels of Oaz1, Oaz2, and Odc expression in mouse hypothalamus (p < 0.05). Treatment with spermidine at 0.05 mg/g significantly increased the levels of Ssat mRNA expression and reduced the level of Smo mRNA expression in mouse hypothalamus (p < 0.05). Putrescine concentrations in the hypothalamus after the administration of spermidine at 0.10 and 0.15 mg/g were significantly higher than those in the control group (p < 0.05). The concentration of both spermidine and spermine in the hypothalamus after the administration of spermidine at 0.15 mg/g was decreased significantly (p < 0.05). In summary, our results indicate that exogenous spermidine affects polyamine homeostasis in the mouse hypothalamus by modulating the expression of genes and proteins related to polyamine metabolism.

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Section: Methodsmentioning

confidence: 99%

Exogenous spermidine affects polyamine metabolism in the mouse hypothalamus

Jiang

et al. 2021

Open Life Sciences

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“…Female laying geese at the age of 7 months were killed by cervical dislocation. Follicle tissues and primary granulosa cells were quickly removed and processed as previously described [ 8 , 22 ]. In brief, granulosa cells were cultured in a DMEM/F12 medium supplemented with 3.0% FBS and 100 U/ml of penicillin/streptomycin in a humidified incubator at 37°C and 5.0% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Several studies have suggested that numerous ROS are produced by the ovaries in female animals during folliculogenesis and ovulation [ 5 , 6 ]. Granulosa cells, which surround oocytes in ovaries, play a pivotal role in follicular development, ovulation, atresia and steroidogenesis [ 7 , 8 ]. It is well known that oxidative stress induces granulosa cell apoptosis, which plays a vital role in initiating follicular atresia [ 9–11 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese

Kang

Wang

et al. 2018

Bioscience Reports

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The mechanism of action by which oxidative stress induces granulosa cell apoptosis, which plays a vital role in initiating follicular atresia, is not well understood. In the present study, the effect of 3-nitropropionic acid (3-NPA) on oxidative stress and apoptosis in granulosa cells in geese was investigated. Our results showed that treatment with 3-NPA at 5.0 mmol/l for 24 h increased intracellular reactive oxygen species (ROS) production by 25.4% and decreased granulosa cell viability by 45.5% (P<0.05). Catalase and glutathione peroxidase gene expression levels in granulosa cells treated with 3-NPA were 1.32- and 0.49-fold compared with those of the control cells, respectively (P <0.05). A significant decrease in the expression level of B-cell lymphoma 2 (Bcl-2) protein and remarkable increases in the levels of Bax, p53 and cleaved-Caspase 3 proteins and the ratio of Bax/Bcl-2 expression in granulosa cells treated with 3-NPA were observed (P<0.05). Furthermore, a 38.43% increase in the percentage of early apoptotic cells was also observed in granulosa cells treated with 3-NPA (P<0.05). Moreover, the expression levels of NF-κB, Nrf2, Fhc, Hspa2 and Ho-1 in granulosa cells treated with 3-NPA were elevated 4.36-, 1.63-, 3.62-, 27.54- and 10.48-fold compared with those of the control cells (P<0.05), respectively. In conclusion, the present study demonstrates that treatment with 3-NPA induces ROS production and apoptosis and inhibits the viability of granulosa cells in geese. Furthermore, 3-NPA triggers increases in the expression of cleaved-Caspase 3 protein and the ratio of Bax/Bcl-2 expression, and induces the early apoptosis of granulosa cells.

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“…), whereas the OAZ1 ( ornithine decarboxylase antizyme 1 ) gene inhibits this process (Kang et al . ).…”

Section: Introductionmentioning

confidence: 97%

Comparative transcriptome analysis reveals genes related to the yolk ratio of duck eggs

Ren

et al. 2019

Animal Genetics

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Yolk ratio is an important production index in the salted duck egg industry. Yolk constituents are deposited during development of follicles. However, the molecular mechanism responsible for different yolk ratios in laying ducks remains elusive. In this study, Shaoxing ducks laying eggs with different yolk ratios were chosen for an analysis of liver and ovary transcriptome information. Twelve libraries were constructed and generated an average of 58.5 million clean reads per library, of which 69% of clean reads from liver and 65% of clean reads from ovary were mapped to a reference genome. Between crossphenotype groups, a total of 250 and 230 differently expressed genes (DEGs) were identified in liver and ovary respectively, of which 101 and 50 DEGs respectively were characterized. Several DEGs were detected, among which HMGCS1, HMGCR, FDFT1, (DHCR7), (STARD4), CYP46A1 and LPIN3 are involved in cholesterol metabolism-related pathways; KIAA0319, STARD4, AP1S3, SH3GL2 and CAV2 are involved in vesicular transport in the liver; and ELOVL2 and PSD2 are involved in fatty acid elongation and endocytosis in the ovary. High yolk-ratio ducks had higher activity for cholesterol synthesis and molecular trafficking. The identification of candidate genes greatly advances the understanding of the genetic basis of the formation of different yolk ratios. ImplicationsRNA-Seq is a useful tool to measure gene transcription and better understand the physiology behind specific phenotypes. The current study enriches the genes that affect yolk ratio in both liver and ovary of laying duck, which could lead to future breeding strategies.

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OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese

Cited by 11 publications

References 42 publications

Exogenous spermidine affects polyamine metabolism in the mouse hypothalamus

Exogenous spermidine affects polyamine metabolism in the mouse hypothalamus

Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese

Comparative transcriptome analysis reveals genes related to the yolk ratio of duck eggs

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