Obituary on Dr. James Bryan Finean: 1928–1993

Chapman, D.

doi:10.1016/0009-3084(94)02321-2

Cited by 2 publications

(1 citation statement)

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“…IFNT and IFN-α have a common receptor in bovine endometrial tissue, and there is no unique IFNT-binding receptor in endometrium [17]. It is through activating Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway that IFNT exerts its effects on the bovine endometrium [18].…”

Section: Introductionmentioning

confidence: 99%

Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

Wang

Han

Zhang

et al. 2019

Animals

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Simple SummaryInterferon-tau is a maternal recognition factor in ruminants, and spleen plays an essential role in regulating innate and adaptive immune responses. We found that interferon-tau derived from conceptus induces expression of STAT1, OAS1, and CXCL10 in ovine maternal spleen, which may be helpful for maternal immune regulation.AbstractInterferon-tau is a maternal recognition factor in ruminant species, and spleen plays an essential role in regulating innate and adaptive immune responses. However, it is not fully understood that early pregnancy induces expression of interferon stimulated genes (ISGs) in the spleen during early pregnancy in ewes. In this study, spleens were collected from ewes at day 16 of the estrous cycle, and on days 13, 16, and 25 of gestation (n = 6 for each group), and RT-qPCR, western blot and immunohistochemistry analysis were used to detect the expression of signal transducer and activator of transcription 1 (STAT1), 2′,5′-oligoadenylate synthetase 1 (OAS1), myxovirusresistance protein 1 (Mx1) and C-X-C motif chemokine 10 (CXCL10). The results revealed that STAT1, OAS1 and CXCL10 mRNA and proteins were upregulated in the spleens during early pregnancy, and STAT1 protein was located in connective tissue cells in the capsule and trabeculae, and blood cells and lymphocytes in the red pulp. However, early pregnancy had no significant effects on expression of MX1 mRNA and protein. In conclusion, early pregnancy induces expression of STAT1, OAS1 and CXCL10 in maternal spleen, suggesting that maternal spleen is involved in immune regulation of pregnancy in sheep.

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Section: Introductionmentioning

confidence: 99%

Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

Wang

Han

Zhang

et al. 2019

Animals

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Ame‐miR‐1‐3p of bee venom reduced cell viability through the AZIN1/OAZ1‐ODC1‐polyamines pathway and enhanced the defense ability of honeybee (Apis mellifera L.)

Liu,

Tian,

Wen

et al. 2024

Insect Molecular Biology

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Bee venom serves as an essential defensive weapon for bees and also finds application as a medicinal drug. MicroRNAs (miRNAs) serve as critical regulators and have been demonstrated to perform a variety of biological functions. However, the presence of miRNAs in bee venom needs to be confirmed. Therefore, we conducted small RNA sequencing and identified 158 known miRNAs, 15 conserved miRNAs and 4 novel miRNAs. It is noteworthy that ame‐miR‐1‐3p, the most abundant among them, accounted for over a quarter of all miRNA reads. To validate the function of ame‐miR‐1‐3p, we screened 28 candidate target genes using transcriptome sequencing and three target gene prediction software (miRanda, PITA and TargetScan) for ame‐miR‐1‐3p. Subsequently, we employed real‐time quantitative reverse transcription PCR (qRT‐PCR), Western blot and other technologies to confirm that ame‐miR‐1‐3p inhibits the relative expression of antizyme inhibitor 1 (AZIN1) by targeting the 3′ untranslated region (UTR) of AZIN1. This, in turn, caused ODC antizyme 1 (OAZ1) to bind to ornithine decarboxylase 1 (ODC1) and mark ODC1 for proteolytic destruction. The reduction in functional ODC1 ultimately resulted in a decrease in polyamine biosynthesis. Furthermore, we determined that ame‐miR‐1‐3p accelerates cell death through the AZIN1/OAZ1‐ODC1‐polyamines pathway. Our studies demonstrate that ame‐miR‐1‐3p diminishes cell viability and it may collaborate with sPLA2 to enhance the defence capabilities of honeybees (Apis mellifera L.). Collectively, these data further elucidate the defence mechanism of bee venom and expand the potential applications of bee venom in medical treatment.

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Obituary on Dr. James Bryan Finean: 1928–1993

Cited by 2 publications

References 0 publications

Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

Ame‐miR‐1‐3p of bee venom reduced cell viability through the AZIN1/OAZ1‐ODC1‐polyamines pathway and enhanced the defense ability of honeybee (Apis mellifera L.)

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