Observations on Sahli's Hæmoglobinometer

Alstead, Stanley

doi:10.1136/pgmj.16.178.278

Cited by 7 publications

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“…Fieldwork teams were trained to measure height (to 1 mm; stadiometer), weight (to 0·1 kg; electronic scales, calibrated daily) and finger-prick haemoglobin 15 . Blood and faecal samples were collected for central retinol assay 16 and central formalin-ether-concentration helminth egg assay 17 (useable only after mid-study, when egg-counting methodology greatly improved).…”

Section: Methodsmentioning

confidence: 99%

Population deworming every 6 months with albendazole in 1 million pre-school children in north India: DEVTA, a cluster-randomised trial

et al. 2013

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SummaryBackgroundIn north India many pre-school children are underweight, many have intestinal worms, and 2–3% die at ages 1·0–6·0 years. We used the state-wide Integrated Child Development Service (ICDS) infrastructure to help to assess any effects of regular deworming on mortality.MethodsParticipants in this cluster-randomised study were children in catchment areas of 8338 ICDS-staffed village child-care centres (under-5 population 1 million) in 72 administrative blocks. Groups of four neighbouring blocks were cluster-randomly allocated in Oxford between 6-monthly vitamin A (retinol capsule of 200 000 IU retinyl acetate in oil, to be cut and dripped into the child's mouth every 6 months), albendazole (400 mg tablet every 6 months), both, or neither (open control). Analyses of albendazole effects are by block (36 vs 36 clusters). The study spanned 5 calendar years, with 11 6-monthly mass-treatment days for all children then aged 6–72 months. Annually, one centre per block was randomly selected and visited by a study team 1–5 months after any trial deworming to sample faeces (for presence of worm eggs, reliably assessed only after mid-study), weigh children, and interview caregivers. Separately, all 8338 centres were visited every 6 months to monitor pre-school deaths (100 000 visits, 25 000 deaths at age 1·0–6·0 years [the primary outcome]). This trial is registered at ClinicalTrials.gov, NCT00222547.FindingsEstimated compliance with 6-monthly albendazole was 86%. Among 2589 versus 2576 children surveyed during the second half of the study, nematode egg prevalence was 16% versus 36%, and most infection was light. After at least 2 years of treatment, weight at ages 3·0–6·0 years (standardised to age 4·0 years, 50% male) was 12·72 kg albendazole versus 12·68 kg control (difference 0·04 kg, 95% CI −0·14 to 0·21, p=0·66). Comparing the 36 albendazole-allocated versus 36 control blocks in analyses of the primary outcome, deaths per child-care centre at ages 1·0–6·0 years during the 5-year study were 3·00 (SE 0·07) albendazole versus 3·16 (SE 0·09) control, difference 0·16 (SE 0·11, mortality ratio 0·95, 95% CI 0·89 to 1·02, p=0·16), suggesting absolute risks of dying between ages 1·0 and 6·0 years of roughly 2·5% albendazole versus 2·6% control. No specific cause of death was significantly affected.InterpretationExisting ICDS village staff can be organised to deliver simple pre-school interventions sustainably for many years at low cost, but regular deworming had little effect on mortality in this lightly infected pre-school population.FundingUK Medical Research Council, USAID, World Bank (albendazole donated by GlaxoSmithKline).

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Section: Methodsmentioning

confidence: 99%

Population deworming every 6 months with albendazole in 1 million pre-school children in north India: DEVTA, a cluster-randomised trial

et al. 2013

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“…Haematological parameters : Blood haemoglobin level was determined according to the Sahli's method 13 . Haematological parameters, i.e ., red blood cell (RBC), white blood cell (WBC) and platelet were measured by automated haematology analyzer (KX-21, Sysmex, Japan).…”

Section: Methodsmentioning

confidence: 99%

Therapeutic potential of xanthones from Swertia chirata in breast cancer cells

Barua

Choudhury

Mandal

et al. 2020

Indian Journal of Medical Research

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Background & objectives : Medicinal plants like Swertia chirata are rich sources of different xanthones. This study was aimed to assess the cytotoxic potential of four most abundant xanthones present in S. chirata both in vivo and in vitro in Ehrlich ascites carcinoma (EAC), a mouse transplantable breast carcinoma cell line and two human breast carcinoma cell lines (MCF-7 and MDA-MB-231). Methods : Four xanthones derived from S. chirata namely 1-hydroxy-3,7,8-trimethoxyxanthone (XA), 1,8-dihydroxy-3,5-dimethoxyxanthone (XB), 1-hydroxy-3,5,8-trimethoxyxanthone (XC) and 1,5,8-trihydroxy-3-methoxyxanthone (XD) were used for determination of sub-lethal dose on the cell lines EAC, MCF-7, MDA-MB-231 and verified toxicity of sub-lethal dose on normal murine fibroblast cells. Cytotoxicity was measured in vivo and survivability of mice was plotted accordingly. Therapeutic efficacy of XD was evaluated both in vivo and in vitro by determination of lipid peroxidation (LPO), reactive oxygen species (ROS) generation and by quantitating the enzyme status (GSH, catalase, superoxide dismutase) in treated and untreated samples. DNA damage was evaluated using comet and DNA fragmentation assays. Furthermore, apoptotic effect was analyzed by flow cytometry and validated by TUNEL assay and Western blotting. Results : Among all the xanthones tested XD showed IC 50 at the lowest dose, and normal cells were unaffected at this dose. Survivability of mice increased significantly when treated with XD compared to other xanthones and cisplatin. Significantly increased ROS and LPO were found in cancer cells as a result of XD treatment which was unaltered in normal cell line. XD induced DNA damage and apoptosis in cancer cell lines. Interpretation & conclusions : Our experimental data indicate that XD may potentially act as a chemotherapeutic agent by enhancing ROS in breast cancer cells thereby leading to apoptosis.

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“…Sahli's haemoglobinometer is a tool used to assess the amount of hemoglobin in the blood (Alstead, 1940). In action the Swiss scientist H invented a hemoglobinometer in 1902.…”

Section: Samplesmentioning

confidence: 99%

Comparison of Hb and PCV Values in Manual Methods – A Prospective Study

Ananya¹

2020

Biosci. Biotech. Res. Comm

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Hemoglobin iron-containing metalloprotein expressed in red for all vertebrate blood cells (erythrocytes), as well as some invertebrate tissues. A healthy person has 12 to 20 grams of hemoglobin in every 100 milliliters of blood. Hematocrit is a blood test measuring the percentage concentration of red blood cells (RBC) in the blood. The measurement will depend on the number and size of cells in red bloods. It is part of the complete results of a person's blood count, along with hemoglobin concentration, white blood cell count, and platelet count. The volume of packed cells (PCV) is a measure of the proportion of blood formed by cells. The value is expressed as one percentage or fraction of blood cells. For example, a 40 per cent PCV means that in 100 milliliters of blood there are 40 milliliters of cells. This study is to compare the Hb and PCV values derived from direct manual and automated methods. Nowadays PCV values are estimated through automated methods. If there are any electrical supply problems, low budget labs, unavailability of the wintrobe's tube, in a practical college set up we have sahli's haemoglobinometer , with this we can be able to calculate the PCV valve. But our results show that the automated PCV values are higher than the manual calculated PCV values and the automated methods are more accurate to follow. Also we found that the manual calculated PCV method is not a better alternative for Automated PCV method.

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Observations on Sahli's Hæmoglobinometer

Cited by 7 publications

References 0 publications

Population deworming every 6 months with albendazole in 1 million pre-school children in north India: DEVTA, a cluster-randomised trial

Population deworming every 6 months with albendazole in 1 million pre-school children in north India: DEVTA, a cluster-randomised trial

Therapeutic potential of xanthones from Swertia chirata in breast cancer cells

Comparison of Hb and PCV Values in Manual Methods – A Prospective Study

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