Observations on the application to electron microscopy of the lead phosphate technique for the demonstration of acid phosphatase

Ericsson, Lennart; Trump, Benjamin F.

doi:10.1007/bf00281900

Cited by 92 publications

(16 citation statements)

References 22 publications

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“…Group A1-2 rats were sacrificed after 5 minutes and A3-6 rats after 7.5, 10, 20, and 30 minutes, respectively. NSG was counted, the diameter of the entire granule and the diameter of the granule core were determined, and acid phosphatase activity was demonstrated employing a modified Gomori method (Ericsson and Trump, 1965).…”

Section: Effect Of High Serum Calcium Levels Onmentioning

confidence: 99%

“…NSG per PT cell section was estimated, and the mean diameter of the core of SGs and the mean surface area of the Golgi region (which is occupied by Golgi cisternae, vacuoles, and vesicles) were measured using a scaled magnification glass x 7 (Peak, Tokyo). Acid phosphatase reaction employing a modified Gomori method (Ericsson and Trump, 1965) was performed in parathyroids of control rats and of rats sacrificed 10 minutes after injection of pilocarpine. After pilocarpine treatment, NSG-I gradually decreased and reached a minimum at 30 minutes (Fig.…”

Section: Effect Of Parasympathetic Stimulation Onmentioning

confidence: 99%

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The biological significance of storage granules in rat parathyroid cells

Setoguti

Inoue

Wild

1995

Microscopy Res & Technique

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Both prosecretory and storage granules are concomitantly formed at the trans Golgi network including the innermost Golgi cisterna. Prosecretory granules develop into small secretory granules that release their contents by exocytosis finely regulated by a complex mechanism for maintaining calcium homeostasis. In the rat parathyroid cells, storage granules are large secretory granules storing parathyroid hormone for an emergency supply. The hormone is rapidly discharged by exocytosis when serum calcium concentration is decreased. The granules are constantly produced even under conditions of low serum calcium concentration in the regions of 8 mg/dl. The granule content is constantly hydrolyzed when not discharged, leading to a decreased core and finally to the formation of vacuolar bodies. The fate of the vacuolar bodies is unknown. Hypercalcemic conditions accelerate hydrolysis. The threshold value of calcium concentration required for the release of storage granule contents is between 8.0 and 7.5 mg/dl and that of calcium concentration for accelerating degradation of storage granules is about 11.5 mg/dl. Sympathetic stimulation causes storage granules to be discharged regardless of hypercalcemia or hypocalcemia. Parasympathetic stimulation accelerates hydrolysis. The degradation of storage granules seems to be closely associated with an intracellular regulatory mechanism for parathyroid hormone secretion.

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Section: Effect Of High Serum Calcium Levels Onmentioning

confidence: 99%

Section: Effect Of Parasympathetic Stimulation Onmentioning

confidence: 99%

The biological significance of storage granules in rat parathyroid cells

Setoguti

Inoue

Wild

1995

Microscopy Res & Technique

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“…acid phosphatase. The presence of acid phosphatase can explain the concentration and local precipitation of elements detected with phosphorus (Ericsson & Trump 1965, Galle & Berry 1980, Haguenoer & Furon 1981 Acid phosphatase enzyme activity can liberate phosphate ions from substrates composed of phosphoric esters (Borel et al 1987). The phosphate ions are then able to precipitate a certain number of mineral elements to form precipitates of insoluble phosphate (Galle & Berry 1980, Galle 1981.…”

Section: Renal Tissue Cellsmentioning

confidence: 99%

Selective intra-lysosomal concentration of niobium in kidney and bone marrow cells: a microanalytical study

1993

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Niobium is used as an alloy in the industrial and biomedical fields. The concentration of the toxic element in organs of a number of animal species has been defined by using radioactive niobium (95Nb). However, tissue lesions induced by niobium have only been studied at the light microscopy level. In this study, we used an electron probe X-ray analyzer equipped with a transmission electron microscope to define the localization of this element in kidney and bone marrow cells. Results demonstrated that niobium is located in the lysosome and that this element coprecipitates with phosphate. In kidney, lysosomes and precipitates are eliminated in the tubular lumen. In contrast, precipitates appear to be eliminated more slowly from the lysosomes of bone marrow macrophages. These processes therefore correspond to one of the mechanisms by which lysosomes eliminate certain toxic mineral elements and thus play a role in the more general process of the body's defenses.

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“…Tissue sections (Mcllvain). 50-80 pm thick, were processed for the demonstration of acid phosphatase activ ity using the method of Ericsson and Trump [ 1965]. For demonstration of the peroxidase activity, we used the cytochemical method of Attgermiiller and Fahimi 119811.…”

Section: Electron Microscopy and Cytochemistrymentioning

confidence: 99%