Observations on the combined effects of light, NAA and 2,4-D on somatic embryogenesis of cucumber (Cucumis sativus) hybrids

Elmeer, Khaled; Hennerty, M. J.

doi:10.1007/s11240-008-9439-0

Cited by 8 publications

(4 citation statements)

References 5 publications

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“…The significance and role of these growth regulators in the somatic embryogenesis of cucumber have also been considered by Khalili et al (2000), Mashayekhi et al (2012), Elmeer and Hennerty (2008), Von Arnold et al (2002) which is consistent with the results of this study. The role of 2,4-dichlorophenoxyacetic acid, as an important and constant growth regulator (and often necessary for the induction of embryogenesis), was reported by Cade et al (1990), Smith (2012) and Ju et al (2014).…”

Section: Discussionsupporting

confidence: 93%

A comparative study on the effects of ultrasound and some growth factors on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.)

Koochani

Majd

Arbabian

et al. 2020

Not Bot Horti Agrobo

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Cucumber (Cucumis sativus L.), a popular vegetable crop of the family Cucurbitaceae, is cultivated in different parts of the world and is of great economic importance. Ultrasound is known as a physical stimulus that can affect living systems. At the right frequency and exposure period, these waves have desired effects. In the present study, 2-week-old plants, were put in an ultrasonic bath with a nominal frequency of 40 kHz, a central frequency of 34/722 kHz and a bandwidth of 320 Hz for 0, 5, 10 and 15 minutes. Hypocotyl pieces were used as explants and cultured in Murashige and Skoog medium supplemented with 0/5 mg/l each of 2,4-dichlorophenoxyacetic acid, 1-Naphthalene acetic acid, kinetin and 6-benzylaminopurine. The use of ultrasonication at a nominal frequency of 40 kHz for 10 minutes showed better results as compared with the samples treated for 0, 5 and 15 minutes, in terms of the shape, maturity of the embryos, and also the germination of artificial seeds and maturing to flowering stage. Since the cucumber of Esfahan is very famous for its aroma and taste and it is somewhat at risk of extinction, we studied the production of artificial seeds of this valuable plant using somatic embryos and apical buds. This research is the first report on investigation of the positive effect of ultrasound on somatic embryogenesis and artificial seed production. our results clearly showed that this method is a practical method to accelerate seed germination and flowering stage.

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Section: Discussionsupporting

confidence: 93%

A comparative study on the effects of ultrasound and some growth factors on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.)

Koochani

Majd

Arbabian

et al. 2020

Not Bot Horti Agrobo

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show abstract

“…Moreover, previous reports have indicated that the combination of 2,4-D with other auxins improved callus initiation (Debeaujon and Branchard, 1993;Khan et al, 2011;Qin Mao et al, 2006). Once the callus initiation is started, one auxin is sufficient to produce friable callus as in case of cucumber (Elmeer and Hennerty, 2008). Previous studies showed that high levels of auxins are required for the induction of embryogenic callus; however, cytokinins are critical for somatic embryo formation (Palmer and Keller, 2011;Webster et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Callus induction and RAPD analysis of Simarouba glauca DC

Kakuturu¹,

Josekutty²,

Reitzel³

et al. 2014

Afr. J. Biotechnol.

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Callus induction for somatic embryogenesis from Simarouba glauca DC leaf explants of three genotypes (S. glauca 5, S. glauca 19 and S. glauca 21) was studied. Leaf explants (leaf segments from basal, middle and tip of the leaves) were cultured on two types of nutrient media; SGC1 and SGC2. Both media contained Murashige and Skoog (MS) medium with vitamins: 100 mg/L ascorbic acid, 0.5 mg/L 6benzylaminopurine (BAP), 0.5 to 5.0 mg/L NAA (1-napthaleneacetic acid), and 3.0 g/L sucrose. The SGC2 media additionally contained 0.5 to 5.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). SGC2 media generated better callusing response compared to SGC1, thus displaying the importance of using 2,4-D in combination with NAA for callus induction. MS medium containing 2.5 mg/L NAA (SGC1.5) was noted to be the most effective in the initiation of friable embryogenic callus. On the other hand, MS medium containing a combination of 2.0 mg/L NAA and 2.0 mg/L 2,4-D was effective in the early initiation of friable embryogenic callus. In addition, a higher frequency of callus formation was observed from basal leaf segment as compared to that from middle and apical leaf segments. A random amplified polymorphic DNA (RAPD) analysis was also performed to see the genetic differences between the three S. glauca genotypes used in this study. The performance of S. glauca 5 and S. glauca 19 for higher callus frequency over the S. glauca 21 could be attributed to the genotypic differences between these genotypes. Overall, our protocol using SGC 2.4 media yielded optimal results and is suitable for large scale micropropagation of S. glauca.

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“…Trên mẫu cấy lá cây Vải (Litchi chinensis), mô sẹo cảm ứng và tăng sinh tốt ở điều kiện chiếu sáng 16 giờ/ngày [16]. Khối lượng tươi đạt cao nhất khi nuôi cấy mô sẹo phát sinh từ lá cây Cuccumis sativus ở điều kiện chiếu sáng là 16 giờ/ngày [9].…”

Section: ảNh Hưởng Của 24-d Kết Hợp Với Naa Lên Sự Cảm ứNg Và Tăng Sunclassified

Effects of some factors on friable callus induction and cell suspension culture of Panax Vietnamensis Ha et Grushv

Cương¹,

Chien²,

Nam³

et al. 2012

V J Bio

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Observations on the combined effects of light, NAA and 2,4-D on somatic embryogenesis of cucumber (Cucumis sativus) hybrids

Cited by 8 publications

References 5 publications

A comparative study on the effects of ultrasound and some growth factors on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.)

A comparative study on the effects of ultrasound and some growth factors on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.)

Callus induction and RAPD analysis of Simarouba glauca DC

Effects of some factors on friable callus induction and cell suspension culture of Panax Vietnamensis Ha et Grushv

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