Observations on the inactivation of adrenaline by blood and tissues in vitro

Amine oxidase and adrenaline

“…Under these conditions the usual physiological methods of estimating adrenaline are not reliable [Bain, Gaunt & Suffolk, 1937] and chemical methods were therefore preferred. The adrenaline was separated from the organic material by deproteinizing with trichloracetic acid and adsorbing on aluminium hydroxide as described by Shaw [1938]: it was then estimated colorimetrically.…”

The inhibition of adrenaline oxidation by local anaesthetics

“…They suggest that the potentiating action of ephedrine is due to inhibition of the amine oxidase. Bain et al [1937] found that the addition of cocaine did not affect the rate of destruction of adrenaline incubated with liver slices, but they used extremely low concentrations of adrenaline and cocaine. Bayer & Wense [1938] and Wense [1939] claim that cocaine slows the rate of oxidation of adrenaline by tyrosinase, acetaldehyde and succinic acid.…”

“…Physiol. (1940) Bain, Gaunt & Suffolk, 1937;Bayer & Wense, 1938]). Blaschko, Richter & Schlossmann [1937] showed that ephedrine inhibits the amine oxidase which oxidizes the side-chain of adrenaline.…”

Communications

“…Department of Physiology and Pharmacology, University of St Andrews, St Andrews, Fife Bain, Gaunt & Suffolk (1937) and Bain & Batty (1956) observed that adrenaline and noradrenaline entered cat and human erythrocytes during in vitro incubation at 37 'C. Schanker, Nafpliotis & Johnson (1961) concluded that catecholamines and 5-hydroxytryptamine entered human erythrocytes by a process of simple diffusion.…”