Obtaining and Experimental Study of Candida Allergens

Abstract

Due to the need of diagnostics of the widespread allergic diseases connected with infection ofa person by fungi, the question of creation of highly sensitive diagnostic panels of fungal allergens including molds and yeasts is important. Work purpose: creation of experimental technology of preparation of allergens from culture of C. albicans and studying of their physical, chemical and immunobiological properties. For receiving allergens we used the inactivated biomass of clinical strains of Candida albicans. Cultivation was carried out in the original mineral (without protein) CC1 and ML media in liquid and agarized variants with addition of sugar in various concentrations. In samples of allergens we defined amount of protein, carbohydrates, nucleic acids, specific activity in vitro (reaction of degranulation of rat mast cells). It was shown that the content of protein nitrogen varied from 72 to 18900 PNU, carbohydrates from 0,001 to 0,079 mg/ ml depending on physiological properties of a population of C. albicans and cultivation conditions. For determination of specific activity (reaction of rat mast cells degranulation) of samples used sera from patients with a sensitization to fungi. Allergenic activity of preparations in reaction of degranulation of rat mast cells varied from 3% (spontaneous) to 52%.The highest specific activity was shown for samples of the allergens obtained from C. albicans, grown in the ML medium. The results obtained using of 18 samples of preparations, can be a basis for a choice of an optimum technological mode of allocation of allergens from C. albicans.