OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE

Herawati, Maria Marina; Pudjihartati, Endang; Pramono, Suwijiyo; Sulistyaningsih, Endang; Purwantoro, Aziz

doi:10.17503/agrivita-2015-37-2-p178-184

Cited by 10 publications

(7 citation statements)

References 9 publications

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“…The colchicine mutagen has been shown to induce polyploidy in various plants, including Scutellaria baicalensis (Gao et al 2002), Panax ginseng (Kim et al 2004), Artemisia annua (De Jesus, 2003;Banyai et al 2010;Rahman et al 2010) 2017), Codonopsis lanceolata (Kwon et al 2016), Glycyrrhiza glabra var. glandulifera (Bernard et al 2012), and Artemisia cina (Herawati et al 2015). Although colchicine treatment in this study can induce polyploidy in A. cina and also increase leaf and root growth, the results are not significant.…”

Section: Resultscontrasting

confidence: 61%

“…A. cina is another species member of genus Artemisia that also has potential as a medicinal plant and source of bioactive compound (anticancer, antibacterial and antifungal). Moreover, the effect of polyploidization on shoot culture of A. cina using plant growth regulator treatment toward plant growth has also been reported by Herawati et al (2015).…”

Section: Introductionmentioning

confidence: 91%

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Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina

2020

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Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be useful information to develop A. cina to become a medicinal plant.

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Section: Resultscontrasting

confidence: 61%

Section: Introductionmentioning

confidence: 91%

Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina

2020

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“…The content of artemisinin in A. annua was considered low; therefore, the induction of the A. annua polyploidy plant is an important stage for increasing artemisinin production [15]. In medicinal plants, leaves are often desired as a source of active compounds, so the increase in biomass associated with polyploid plants is very demanding.…”

Section: Discussionmentioning

confidence: 99%

Artemisinin Content on Artemisia Annua L. Treated by Gloriosa Superba Seeds’ Water Extract

Susilowati

Widyastuti³

et al. 2018

Asian J Pharm Clin Res

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Objective: The aim of this study was to determine the artemisinin content on Artemisia annua L. treated by water extract of Gloriosa superba seeds.Methods: G. superba seeds obtained naturally on Krakal Beach, Gunung Kidul, and extraction used a maceration method by water solvent (1:1). A. annua L. sprouts were obtained from B2P2TOOT Tawangmangu. Treatment variables done on sprouts using various water extract concentration of G. superba seeds and soaking time on A. annua L. sprouts. Determination of artemisinin content in leaf extract of A. annua L. was done using KLT-densitometric method with n-hexane:ethyl acetate (4:1) as mobile phase.Result: The result showed that artemisinin content in plant treatment of G. superba seed water extract was higher (9.78 μg/μl [±3.21]–16.60 μg/μl [±1.39]) compared to control plants (6.39 μg/μl [±1.40]). The concentration water extract of G. superba seed affected the level of artemisinin in the treatment plant. On the other hand, the soaking of A. annua L. sprouts using the water extract of G. superba seed did not affect the level of artemisinin content.Conclusion: Artemisinin content in treatment plant by G. superba seed water extract treatment was higher compared to control plants.

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“…(Rantau et al, 2014). Herawati, et al (2015) stated that leaf stomatal density was strongly influenced by ploidy level. The polyploid plants had low stomatal density.…”

Section: The Size Of Artemisia Annua Stomatal Soaked In Water Extractmentioning

confidence: 99%

Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds

2017

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Artemisia annua is a herbaceous plant that produces artemisinin as a malaria drug, haemorrhoids therapy, aromatherapy, antiviral, anticancer and antibacterial. Gloriosa superba is a plant that contains high colchicine compounds, especially on the seeds. Gloriosa superba extracts of tubers, stems, seeds, and leaves were used as biomutagen for many plants. Colchicine contains of these plants as antimitotic have been studied and proven by the mitotic index plants. Water extracts of Gloriosa superba seeds was used as a mutagen for Artemisia annua. The aim of this study was to determine the size and density of Artemisia annua stomata soaked in water extract of Gloriosa superba seeds as a mutagen. Extraction of Gloriosa superba seeds obtained naturally on Krakal Beach, Gunung Kidul by using a maceration method with water solvent (1:1). Artemisia annua sprouts were obtained from B2P2TOOT Tawangmangu. Variables treatment on sprouts using water extract concentration of Gloriosa superba seeds and soaking time of Artemisia annua sprouts. Measurements of stomatal length, width and density were conducted in epidermis of Artemisia annua leaf. Observation and measurements of the stomata were conducted by using a light microscope. The results showed that the length and width of stomata were 0.025 mm and 0.017 mm respectively. The stomatal density of the control leaf (174.69 amount/mm2) was lower than the other treated plants. Stomatal size and density has increased with the increasing concentration extracts on treated plants. Water extracts of Gloriosa superba seeds proved the effects on stomatal size and density of treated plants.

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OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE

Cited by 10 publications

References 9 publications

Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina

Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina

Artemisinin Content on Artemisia Annua L. Treated by Gloriosa Superba Seeds’ Water Extract

Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds

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