2010
DOI: 10.1134/s1607672910040113
|View full text |Cite
|
Sign up to set email alerts
|

Obtaining tomato plants transgenic for the preS2-S-HDEL gene, which synthesize the major hepatitis B surface antigen

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

1
6
1

Year Published

2010
2010
2021
2021

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 10 publications
(8 citation statements)
references
References 11 publications
1
6
1
Order By: Relevance
“…However, VLP-analogues (VLPs for simplification) formed by M- and L-HBsAg are significantly less stable than the actual VLPs of S-HBsAg. It could explain the several times lower level of M/L-HBsAg VLPs, observed in this study as well as previously (Ehsani et al 1997 ; Joung et al 2004 ; Lou et al 2007 ; Salyaev et al 2010 ) in comparison to tens of micrograms of S-HBsAg VLPs (Pniewski et al 2011 ; Richter et al 2000 ). In accordance to lower stability of VLPs assembled by M/L-HBsAg, the antigens exist mainly in small-size forms, probably monomers or dimers, visualised as the total antigen content excluding VLPs (Fig.…”
Section: Discussionsupporting
confidence: 90%
See 1 more Smart Citation
“…However, VLP-analogues (VLPs for simplification) formed by M- and L-HBsAg are significantly less stable than the actual VLPs of S-HBsAg. It could explain the several times lower level of M/L-HBsAg VLPs, observed in this study as well as previously (Ehsani et al 1997 ; Joung et al 2004 ; Lou et al 2007 ; Salyaev et al 2010 ) in comparison to tens of micrograms of S-HBsAg VLPs (Pniewski et al 2011 ; Richter et al 2000 ). In accordance to lower stability of VLPs assembled by M/L-HBsAg, the antigens exist mainly in small-size forms, probably monomers or dimers, visualised as the total antigen content excluding VLPs (Fig.…”
Section: Discussionsupporting
confidence: 90%
“…Anti-HBs immune response, both in mice and humans, was induced exclusively by oral administration of plant material containing S-HBsAg (Kapusta et al 1999 , 2001 ; Pniewski et al 2011 ), although in most cases oral immunisation was combined with the parenteral priming or boosting using purified HBsAg (Joung et al 2004 ; Kong et al 2001 ; Mason et al 2003 ; Richter et al 2000 ; Thanavala et al 2005 ). In comparison, M- and L-HBsAg were expressed in plant systems, such as potato tubers or tomato fruits, only in a few experiments and at levels of tens or hundreds of ng/g FW (Ehsani et al 1997 ; Lou et al 2007 ; Salyaev et al 2010 ). Plant-associated M-HBsAg was also able to induce some immune response after immunisation conducted in oral priming and injection boosting patterns (Joung et al 2004 ; Youm et al 2007 ).…”
Section: Introductionmentioning
confidence: 99%
“…The middle surface antigen was expressed in transgenic potato [37,44], tomato [65,66] and transiently in N. benthamiana [61,62], while the large surface antigen was expressed in tomato [67], and both antigens in tobacco and lettuce [31]. Similar to S-HBsAg, different expression cassettes were examined, both simple ones containing the regular 35S promoter and an unmodified antigen coding sequence [31,37,65], and optimized ones including enhanced or organ-specific promoters [44,67], viral transcription activators [44,61,62] or signal peptides [61,62,66,67]. Production efficiency of M- and L-HBsAg varied from 10 to 100 ng/mg TSP or 0.4–2 μg/g FW [37,44,61,62,6567], exceptionally reaching ≥ 10 μg/g FW [31].…”
Section: Production Of Hbv Antigens In Plant Systemsmentioning
confidence: 99%
“…It is able to mediate HBV adhesion and the invasion of liver cells, and with strong immunogenicity, is able to induce the generation of neutralizing antibodies and protective immunity. Containing the specific T and B lymphocytes binding site, it is able to break the body immune tolerance of existing HBsAg vaccine (10)(11)(12).…”
Section: Discussionmentioning
confidence: 99%