Occlusal disharmony induces BDNF level in rat submandibular gland

Irie, Koichiro; Ekuni, Daisuke; Tomofuji, Takaaki; Azuma, Tetsuji; Endo, Yoshikatsu; Kasuyama, Kenta; Morita, Manabu

doi:10.1016/j.archoralbio.2010.09.001

Cited by 14 publications

(15 citation statements)

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“…Occlusal disharmony induces psychological stress (Yoshihara et al 2001;Onozuka et al 2002;Irie et al 2011;Ekuni et al 2011). Psychological stress Fig.…”

Section: Discussionmentioning

confidence: 98%

Occlusal Disharmony Increases Amyloid-β in the Rat Hippocampus

et al. 2011

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Amyloid-β plays a causative role in Alzheimer's disease. Occlusal disharmony causes chronic psychological stress, and psychological stress increases amyloid-β accumulation. The purpose of the present study was to investigate whether occlusal disharmony-induced psychological stress affects the accumulation of amyloid-β and its related gene expressions in the rat hippocampus. Eight-week-old male Wistar rats (n = 18) were divided into three groups of six rats each: (1) a control group that received no treatment for 8 weeks; (2) an occlusal disharmony group that underwent cutoff maxillary molar cusps for 8 weeks; and (3) a recovered group that underwent cutoff maxillary molar cusps for 4 weeks followed by recovery for 4 weeks. Occlusal disharmony increased plasma corticosterone levels in a time-dependent manner. Levels of amyloid-β 40 and 42, glucocorticoid receptor (Gr) protein, and cleaved caspase 3 (Casp3) as well as gene expressions of amyloid precursor protein, beta-secretase, Casp3, and Gr in the hippocampus in the occlusal disharmony group were significantly higher than those in the control group (P < 0.016). These findings were significantly improved by recovery of occlusion (P < 0.016). These results indicate that psychological stress induced by occlusal disharmony reversibly induces amyloid-β 40 and 42 in the rat hippocampus through the glucocorticoid signal.

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“…Occlusal disharmony induces psychological stress (Yoshihara et al 2001;Onozuka et al 2002;Irie et al 2011;Ekuni et al 2011). Psychological stress Fig.…”

Section: Discussionmentioning

confidence: 98%

Occlusal Disharmony Increases Amyloid-β in the Rat Hippocampus

et al. 2011

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“…Plasma samples were collected from the heart at 8 weeks, between 7:00 am and 9:00 am, in tubes containing EDTA (Terumo, Tokyo, Japan) [27]. Tubes were immediately placed on ice and then centrifuged at 2,000 × g for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Occlusal disharmony accelerates the initiation of atherosclerosis in apoE knockout rats

et al. 2014

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BackgroundPsychosocial stress is one of the risk factors for atherosclerosis. As occlusal disharmony induces psychological stress, we hypothesized that psychological stress by occlusal disharmony accelerates atherosclerosis. The aim of this study was to investigate the effects of occlusal disharmony on the initiation of atherosclerosis in apolipoprotein E (apoE) knockout rats.MethodsFourteen male apoE-knockout rats (age; 8 weeks) (Sprague–Dawley strain background) were divided into two groups of seven rats: the occlusal disharmony group and the no treatment (control) group. In the occlusal disharmony group, the maxillary molar cusps were cut off for the 8-week experimental period.ResultsIn the occlusal disharmony group, the percentages of the area of total aortic lumen occupied by plaques and lipid were significantly higher than those in the control group (p < 0.05, t-test). The occlusal disharmony group also showed significantly higher serum levels of very low-density lipoprotein-cholesterol (VLDL) and low-density lipoprotein-cholesterol (LDL), plasma levels of corticosterone (1.9, 1.3 and 1.3 times, respectively), higher aortic protein expression levels of vascular cell adhesion molecule-1 (VCAM1) and intercellular adhesion molecule-1 (ICAM1) (1.5 and 1.4 times, respectively), and higher aortic gene expression of levels of VCAM1 and Toll-like receptor 4 (TLR4) (1.9 and 4.3 times, respectively), as compared to the control group (p < 0.05). However, there were no significant differences in serum levels of oxidized LDL, reactive oxygen metabolites and C-reactive protein between the two groups.ConclusionIn apoE knockout rats, occlusal disharmony may induce VCAM1, ICAM1 and TLR4 expression and accelerate the initiation of atherosclerosis.

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“…Both genotypes of rats were randomly divided into two groups of six rats each: a control (C) group, which received no treatment for 8 weeks, and an occlusal disharmony (D) group, in which all maxillary molar crowns were cut off for 8 weeks [22,24]. In the D group, the bilateral maxillary molar crowns were cut off at the gingival margin using a dental turbine [22,24].…”

Section: Methodsmentioning

confidence: 99%

“…In the D group, the bilateral maxillary molar crowns were cut off at the gingival margin using a dental turbine [22,24]. Thus, four groups were analyzed in this study: C-WT, D-WT, C-ApoE(−/−), and D-ApoE(−/−).…”

Section: Methodsmentioning

confidence: 99%

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Effects of apoE Deficiency and Occlusal Disharmony on Amyloid-Beta Production and Spatial Memory in Rats

et al. 2013

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Amyloid-β (Aβ) plays a causative role in Alzheimer’s disease. Apolipoprotein E (apoE) is involved in Aβ accumulation, whereas occlusal disharmony increases Aβ production in the rat hippocampus. The purpose of the present study was to investigate the effects of apoE deficiency and occlusal disharmony on Aβ production and spatial memory. Wild-type (WT) (n = 12) and apoE-deficient [ApoE(−/−)] (n = 12) rats (Sprague-Dawley; 8 weeks old) were used. These rats were randomly divided into four groups of six rats each: two control (C) groups: WT (C-WT) and ApoE [C-ApoE(−/−)], and two occlusal disharmony (D) groups: WT (D-WT) and ApoE [D-ApoE(−/−)]. The C group received no treatment for 8 weeks. In the D group, the maxillary molar cusps were cut off for 8 weeks. The spatial memory of rats was assessed according to their behavioral performance in a radial arm maze. In both genotypes of rats, significant differences in the reference memory, Aβ42 production, β-secretase expression and plasma corticosterone levels were observed between the C and D groups (P < 0.0125). The levels of Aβ42 and glucocorticoid receptor in the C-ApoE(−/−) group were also significantly higher than those in the C-WT group (P < 0.0125). However, no significant differences in these parameters were found between the two genotypes with occlusal disharmony. In conclusion, occlusal disharmony induces cognitive dysfunction and Aβ accumulation in the rat hippocampus, and the effects of occlusal disharmony on Aβ accumulation and cognitive dysfunction were larger than those of apoE deficiency.

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Occlusal disharmony induces BDNF level in rat submandibular gland

Abstract: these results indicate that psychological stress induced by occlusal disharmony reversibly induces BDNF expression in the rat submandibular gland.

Cited by 14 publications

References 24 publications

Occlusal Disharmony Increases Amyloid-β in the Rat Hippocampus

Occlusal Disharmony Increases Amyloid-β in the Rat Hippocampus

Occlusal disharmony accelerates the initiation of atherosclerosis in apoE knockout rats

Effects of apoE Deficiency and Occlusal Disharmony on Amyloid-Beta Production and Spatial Memory in Rats

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