Occurrence and composition of class 1 and class 2 integrons in clinical and environmental O1 and non-O1/non-O139 Vibrio cholerae strains from the Brazilian Amazon

Sá, Lena Líllian Canto de; Fonseca, Érica L.; Pellegrini, Maíra; Freitas, Fernanda; Loureiro, Edvaldo Carlos Brito; Vicente, Ana Carolina Paulo

doi:10.1590/s0074-02762010000200021

Cited by 16 publications

(7 citation statements)

References 18 publications

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“…The class 2 integrons detected in one E. coli and in two genetic related R. planticola isolates corresponded to In 2–35 and In 2–11 , already described in E. coli from animals and V. cholerae O1 clinical strain (Kadlec and Schwarz, 2008 ; Sá et al, 2010 ). As noted in this study, class 2 integrons normally show less variability than class 1 and 3 integrons, due to the lack of integration activity of intI2 gene, which is commonly truncated.…”

Section: Discussionsupporting

confidence: 58%

Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables

et al. 2016

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The spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. To understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance Gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, tomatoes, and carrots). Although low levels of resistance have been detected, the bacterial genera identified in the assessed fresh produce are often described not only as environmental, but mostly as commensals and opportunistic pathogens. The genomic characterization of integron-harboring isolates revealed a high number of mobile genetic elements and clinically relevant antibiotic resistance genes, of which we highlight the presence of as mcr-1, qnrA1, blaGES−11, mphA, and oqxAB. The study of class 1 (n = 8), class 2 (n = 3) and class 3 (n = 1) integrons, harbored by species such as Morganella morganii, Escherichia coli, Klebsiella pneumoniae, led to the identification of different integron promoters (PcW, PcH1, PcS, and PcWTNG−10) and cassette arrays (containing drfA, aadA, cmlA, estX, sat, and blaGES). In fact, the diverse integron backbones were associated with transposable elements (e.g., Tn402, Tn7, ISCR1, Tn2*, IS26, IS1326, and IS3) that conferred greater mobility. This is also the first appearance of In1258, In1259, and In3-13, which should be monitored to prevent their establishment as successfully dispersed mobile resistance integrons. These results underscore the growing concern about the dissemination of acquired resistance genes by mobile elements in the food chain.

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Section: Discussionsupporting

confidence: 58%

Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables

et al. 2016

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“…Resistance to streptomycin is often mediated by enzymatic inactivation through adenylation by aminoglycoside (3″) adenyltransferases ( aadA genes) or through phosphorylation by aminoglycoside phosphotransferases ( strA / strB genes; Shaw et al, 1993 ; Tsai et al, 2014 ). However, none of the Vibrio isolates was positive for amplification of aadA1, aadA2 , or strA/B genes that are commonly found in Enterobacteriaceae and that have already been identified in V. cholerae (Hochhut et al, 2001 ; Sá et al, 2010 ; Yu et al, 2012 ). Ribosomal alterations resulting from mutations in the rpsL gene encoding ribosomal protein S12 can be another cause of streptomycin resistance (Shaw et al, 1993 ; Tsai et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Survey on antimicrobial resistance patterns in Vibrio vulnificus and Vibrio cholerae non-O1/non-O139 in Germany reveals carbapenemase-producing Vibrio cholerae in coastal waters

et al. 2015

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An increase in the occurrence of potentially pathogenic Vibrio species is expected for waters in Northern Europe as a consequence of global warming. In this context, a higher incidence of Vibrio infections is predicted for the future and forecasts suggest that people visiting and living at the Baltic Sea are at particular risk. This study aimed to investigate antimicrobial resistance patterns among Vibrio vulnificus and Vibrio cholerae non-O1/non-O139 isolates that could pose a public health risk. Antimicrobial susceptibility of 141 V. vulnificus and 184 V. cholerae non-O1/non-O139 strains isolated from German coastal waters (Baltic Sea and North Sea) as well as from patients and retail seafood was assessed by broth microdilution and disk diffusion. Both species were susceptible to most of the agents tested (12 subclasses) and no multidrug-resistance was observed. Among V. vulnificus isolates, non-susceptibility was exclusively found toward aminoglycosides. In case of V. cholerae, a noticeable proportion of strains was non-susceptible to aminopenicillins and aminoglycosides. In addition, resistance toward carbapenems, quinolones, and folate pathway inhibitors was sporadically observed. Biochemical testing indicated the production of carbapenemases with unusual substrate specificity in four environmental V. cholerae strains. Most antimicrobial agents recommended for treatment of V. vulnificus and V. cholerae non-O1/non-O139 infections were found to be effective in vitro. However, the occurrence of putative carbapenemase producing V. cholerae in German coastal waters is of concern and highlights the need for systematic monitoring of antimicrobial susceptibility in potentially pathogenic Vibrio spp. in Europe.

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“…This work included 13 clinical A. baumannii isolates recovered from Rio de Janeiro (Southeast region) in 2005 and 15 A. baumannii isolates recovered from Maranhão (Northeast region) in 2007 (n = 28). These strains were screened for class 1, 2 and 3 integrons and for their variable cassette region by polymerase chain reaction (PCR) and sequencing using previously described primers [4]. Class 1 integrons, which have a worldwide distribution, were shown to be prevalent in Chile and Argentina [1], contrasting with the current sample where this element was found in only one Brazilian isolate (AC9-RJ).…”

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confidence: 94%

Class 2 integrons in multidrug-resistant Acinetobacter baumannii circulating in different Brazilian geographic regions

Fonseca

Freitas

Scheidegger

et al. 2011

International Journal of Antimicrobial Agents

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Occurrence and composition of class 1 and class 2 integrons in clinical and environmental O1 and non-O1/non-O139 Vibrio cholerae strains from the Brazilian Amazon

Abstract: This study identified and characterised class 1 and 2 integrons in clinical and environmental

Cited by 16 publications

References 18 publications

Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables

Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables

Survey on antimicrobial resistance patterns in Vibrio vulnificus and Vibrio cholerae non-O1/non-O139 in Germany reveals carbapenemase-producing Vibrio cholerae in coastal waters

Class 2 integrons in multidrug-resistant Acinetobacter baumannii circulating in different Brazilian geographic regions

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