Occurrence and Evaluation of Methodologies to Detect Cryptosporidium spp. in Treated Water in the Central-West Region of Brazil

Santos, Sônia F. O.; Silva, Hugo D.; Wosnjuk, Ludimila A. C.; Anunciação, Carlos Eduardo; Silveira-Lacerda, Elisângela de Paula; Peralta, Regina Helena Saramago; Cunha, Flávia S.; Ferreira, Tatiana Dela-Sávia; García-Zapata, Marco Túlio Antônio

doi:10.1007/s12403-015-0187-1

Cited by 6 publications

(1 citation statement)

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“…[ 40 ]. Oocysts of this protozoan were detected by molecular and immunological methods in domestic tap water in the Central-West Region of Brazil [ 41 ]. Among those cockatiels surveyed in this study eating greens, fruits, and vegetables, the most frequently eaten vegetables were lettuce, tomatoes, and carrots.…”

Section: Discussionmentioning

confidence: 99%

Cryptosporidium proventriculi in Captive Cockatiels (Nymphicus hollandicus)

et al. 2023

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Cockatiels (Nymphicus hollandicus) are among the most commonly sold psittacines pets. The aim of this study was to evaluate the occurrence of Cryptosporidium spp. in domestic N. hollandicus and identify risk factors for this infection. We collected fecal samples from 100 domestic cockatiels in the city of Araçatuba, São Paulo, Brazil. Feces from birds of both genders and older than two months were collected. Owners were asked to complete a questionnaire to identify how they handle and care for their birds. Based on nested PCR targeting the 18S rRNA gene, the prevalence of Cryptosporidium spp. in the cockatiels sampled was 9.00%, 6.00% based on Malachite green staining, 5.00% based on modified Kinyoun straining, and 7.00% when the Malachite green was combined with Kinyoun. Applying multivariate logistic regression to test the association between Cryptosporidium proventriculi positivity and potential predictors showed that gastrointestinal alterations was a significant predictor (p < 0.01). Amplicons from five samples were sequenced successfully and showed 100% similarity with C. proventriculi. In summary, this study demonstrates the occurrence of C. proventriculi in captive cockatiels.

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Section: Discussionmentioning

confidence: 99%