Occurrence and spatial distribution of dioxin and dioxin-like compounds in topsoil of Taranto (Apulia, Italy) by GC-MS analysis and DR-CALUX® bioassay

Liberatori, Giulia; Cotugno, Pietro; Sturba, Lucrezia; Vannuccini, Maria Luisa; Capasso, Gennaro; Velardo, Raffaele; Besselink, Harrie; Massari, Federica; Tursi, Angelo; Corbelli, Vera; Behnisch, Peter; Corsi, Ilaria

doi:10.1016/j.chemosphere.2021.130576

Cited by 7 publications

(5 citation statements)

References 55 publications

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“…A study of soil samples taken in China and Korea's coastal areas revealed that the concentrations of PCDD/Fs at the sampling sites in Korea were higher than those in China, but both countries are lower than Japan [52]. Dioxin concentrations have not decreased in Europe in recent years, but instead, have increased significantly due to new dioxins emission sources, such as the illegal disposal of electric transformers in Italy [65] and Sweden [66]. Besides the main sources of pollution from manufacturing industries, motor vehicle emissions are one of the sources of pollution in Russia, because motor fuel combustion is dependent on the dopes used [67].…”

Section: Situation Of Dioxin-contaminated Soil and Standard Limitsmentioning

confidence: 99%

A Review of Soil Contaminated with Dioxins and Biodegradation Technologies: Current Status and Future Prospects

Nhung

Nguyen

Long

et al. 2022

Toxics

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This article provides a comprehensive assessment of dioxins contaminating the soil and evaluates the bioremediation technology currently being widely used, and also offers recommendations for future prospects. Soil pollution containing dioxins is extremely toxic and hazardous to human health and the environment. Dioxin concentrations in soils around the world are caused by a variety of sources and outcomes, but the main sources are from the consequences of war and human activities. Bioremediation technology (bioaugmentation, biostimulation, and phytoremediation) is considered an optimal and environmentally friendly technology, with the goal of applying native microbial communities and using plant species with a high biomass to treat contaminated dioxins in soil. The powerful bioremediation system is the growth of microorganisms that contribute to the increased mutualistic and competitive relationships between different strains of microorganisms. Although biological treatment technology can thoroughly treat contaminated dioxins in soil with high efficiency, the amount of gas generated and Cl radicals dispersed after the treatment process remains high. Further research on the subject is required to provide stricter control over the outputs noted in this study.

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Section: Situation Of Dioxin-contaminated Soil and Standard Limitsmentioning

confidence: 99%

A Review of Soil Contaminated with Dioxins and Biodegradation Technologies: Current Status and Future Prospects

Nhung

Nguyen

Long

et al. 2022

Toxics

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“…A composite topsoil sample (5 kg) made up of 3 sub-samples taken in a 20 m 2 area was obtained using a stainless-steel spade. The resulting topsoil composite sample (60 g) was lyophilized, sieved (2 mm), and extracted (5 g) by accelerated solvent extraction (Dionex ASE 350, Thermo Fisher Scientific) using a standardized protocol previously described [31]. One topsoil extract was tested using the traditional luciferase measurement conducted by following the Dutch standard method RIKZ-SPECIE 07 according to the method reported for soil samples in [31].…”

Section: Experimental Designmentioning

confidence: 99%

“…The resulting topsoil composite sample (60 g) was lyophilized, sieved (2 mm), and extracted (5 g) by accelerated solvent extraction (Dionex ASE 350, Thermo Fisher Scientific) using a standardized protocol previously described [31]. One topsoil extract was tested using the traditional luciferase measurement conducted by following the Dutch standard method RIKZ-SPECIE 07 according to the method reported for soil samples in [31]. The other topsoil extract, after the same procedure of extraction and purification, was used in proteomic analysis.…”

Section: Experimental Designmentioning

confidence: 99%

“…Cells pellets were resuspended for proteomic analysis in 70 µL of lysis buffer (7M urea, 2M thiourea, 4% (w/v) CHAPS, and 1% (w/v) DTE) to extract the whole protein content. Protein concentration was then estimated using Bradford assay [31] before proceeding with two-dimensional electrophoresis. All sample aliquots were stored at −80 • C until use.…”

Section: Rat Hepatoma Cell (H4iie) Preparation For Proteomic Analysismentioning

confidence: 99%

“…The analytical determinations of PCDDs, PCDFs, and dl-PCBs were carried out according to EPA methods 1613B and 1668C, using a Trace™ 1300 gas chromatograph (Thermo Fisher Scientific) coupled to mass spectrometry (TSQ 8000, Triple Quadrupole, Thermo Fisher Scientific) according to the methods already fully described in [31] for soil samples. Estimated concentrations for each detected analyte of PCDD/Fs and dl-PCBs were expressed in terms of toxic equivalency (TEQ), resulting from the product of the concentration and individual toxic equivalent factor (TEF) of each congener [34].…”

Section: Chemical Analysis Of Topsoil Samplesmentioning

confidence: 99%

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First Attempt to Couple Proteomics with the AhR Reporter Gene Bioassay in Soil Pollution Monitoring and Assessment

Landi

Liberatori

Cotugno

et al. 2021

Toxics

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A topsoil sample obtained from a highly industrialized area (Taranto, Italy) was tested on the DR-CALUX® cell line and the exposed cells processed with proteomic and bioinformatics analyses. The presence of polyhalogenated compounds in the topsoil extracts was confirmed by GC-MS/MS analysis. Proteomic analysis of the cells exposed to the topsoil extracts identified 43 differential proteins. Enrichment analysis highlighted biological processes, such as the cellular response to a chemical stimulus, stress, and inorganic substances; regulation of translation; regulation of apoptotic process; and the response to organonitrogen compounds in light of particular drugs and compounds, extrapolated by bioinformatics all linked to the identified protein modifications. Our results confirm and reflect the complex epidemiological situation occurring among Taranto inhabitants and underline the need to further investigate the presence and sources of inferred chemicals in soils. The combination of bioassays and proteomics reveals a more complex scenario of chemicals able to affect cellular pathways and leading to toxicities rather than those identified by only bioassays and related chemical analysis. This combined approach turns out to be a promising tool for soil risk assessment and deserves further investigation and developments for soil monitoring and risk assessment.

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Assessment of receptor-mediated activity (AhR and ERα), mutagenicity, and teratogenicity of metal shredder wastes in Wallonia, Belgium

Matranxhi,

Mertens,

Anthonissen

et al. 2024

Environ Sci Pollut Res

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Occurrence and spatial distribution of dioxin and dioxin-like compounds in topsoil of Taranto (Apulia, Italy) by GC-MS analysis and DR-CALUX® bioassay

Cited by 7 publications

References 55 publications

A Review of Soil Contaminated with Dioxins and Biodegradation Technologies: Current Status and Future Prospects

A Review of Soil Contaminated with Dioxins and Biodegradation Technologies: Current Status and Future Prospects

First Attempt to Couple Proteomics with the AhR Reporter Gene Bioassay in Soil Pollution Monitoring and Assessment

Assessment of receptor-mediated activity (AhR and ERα), mutagenicity, and teratogenicity of metal shredder wastes in Wallonia, Belgium

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