Occurrence, characterization, and antibiogram of Staphylococcus aureus in meat, meat products, and some seafood from Libyan retail markets

Abstract

Aim: The aim of the current investigation was to screen the presence of Staphylococci spp., especially S. aureus in meat, meat products of different animal species, and some seafood sold in some retail markets in Libya using cultural and molecular techniques, and to study their antibiotics resistance profiles. Materials and Methods: A total of 139 samples from red meat, meat products, and seafood were collected from many areas in Libya. Enumeration and isolation of Staphylococci spp. and S. aureus by normal cultural methods followed by molecular identification using molecular techniques by bacterial DNA extraction and partial sequencing of 16S rDNA. Results: Out of 139 samples, 112 (80.6%) were contaminated with different species of Staphylococci based on cultural characteristics of Staphylococci on Baird-Parker medium, for which S. aureus was detected in only 32 samples (23%). However, only six out of 18 (33.3%) isolates sent for sequencing were confirmed to be S. aureus using the molecular technique. The six identified isolates of S. aureus were tested for antimicrobial resistance against 24 most commonly used antibiotics. All isolates were resistant to only two antibiotics (cefotaxime and clindamycin). Among these six isolates, only one confirmed to be Methicillin-resistant Staphylococcus aureus. Conclusion: Results of this study suggest that food of animal origin could be a source of S. aureus with antimicrobial resistance characteristics that can be spread through the food chain, and raise the importance of these results for public health.

Prevalence and Antibiogram Assessment of Staphylococcus aureus in Chicken Carcass Rinse Water at Artisanal Slaughterhouses in Abidjan District, Côte d’Ivoire

“…In another studies, Naas et al [5] showed that six (6) S. aureus isolates were resistant to Clindamycin (100%), tobramycin (50%), Erytromycin (50%), Gentamycin (50%), Penicillin (50%) and tetracyclin (66%) while no resistant strains were detected with trimethoprim + sulfamethoxasole (0%) and Vancomycin (0%). Tassew et al [25] and Waters et al [26] reported high resistance to erythromycin, tetracycline, penicillin in a ratio ranged from 66% to 85%.…”

“…Moreover, the resistance to minocyclin is not surprising because cyclin family is one of the most commonly used antibiotics in Abidjan livestock production systems. In addition, resistance to Vancomycin seems to be low in many reported studies [5]. The difference in the percentage of antibiotic susceptibility may be due to the type of antibiotic used in farming sector or due to local S. aureus strains to each country [5,28].…”

“…In present study, we are interested in the slaughter conditions of poultry in artisanal slaughterhouses in the markets of the district of Abidjan in order to identify the critical points of contamination of the carcass by bacteria potentially pathogenic to consumers. In general, the digestive tract of poultry is one of the reservoirs of many pathogenic bacteria [2,3,4] such as S. aureus [5].…”

A Preliminary Study: Antibiotic Resistance of Escherichia coli and Staphylococcus aureus from the Meat and Feces of Various South African Wildlife Species

“…The presence of S. aureus on the meat samples (36%) indicates that unhygienic practices and thus cross-contamination occurred onto the meat from the hide and/ or from the meat handlers. Ultimately, the presence of S. aureus on raw meat is indicative of poor hygiene conditions in the food chain, mainly due to contamination by food handlers and equipment ( Naas et al, 2019 ). The detection of E. coli and S. aureus from the meat samples was expected and the frequency of isolation is similar to those found by Schlegelová et al (2004) , who detected E. coli and S. aureus in meat samples of beef in a slaughterhouse in 67% and 24% of samples, respectively.…”

Antimicrobial Resistance and Biofilm Encoding Genes Amongst the Staphylococcus aureus Bacteria Isolated From Meat and Meat Products

“…Incidence of S. aureus in meat products in Iran [18], China [19], India [20], USA [21], Iraq [22], and Nigeria [23] were 9.86%, 35%, 21.81%, 27.90%, 54.20%, and 9.70%, respectively. Total incidence of S. aureus amongst the beef, camel meat, chicken, mutton, beef burger, beef sausage, ground beef, chicken burger, ground chicken, and chicken sausage samples collected from Libya were 20%, 23.80%, 40%, 0%, 25%, 33%, 36.40%, 50%, 20%, and 10%, respectively [24]. Brazilian survey [25] revealed that the incidence of S. aureus amongst the beef burger, beef sandwich, chicken burger and chicken sandwich samples were 64%, 20%, 72% and 8%, respectively.…”