Occurrence, characterization and antimicrobial susceptibility of Vibrio alginolyticus in the Eastern Adriatic Sea

Kapetanović, Damir; Irena, Vardić Smrzlić; Valić, Damir; Teskeredžić, Emin

doi:10.1016/j.marpolbul.2013.08.008

Cited by 22 publications

(18 citation statements)

References 24 publications

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“…Main identified causative bacterial agents have been identified as V. anguillarum, V. alginolyticus, V. harveyi or P. damselae spp. piscicida (Romalde, 2002;Pujalte et al, 2003;Toranzo et al, 2005;Austin and Austin, 2007;Damir et al, 2013). These bacteria produce, amongst other lesions, skin ulcers very similar to those observed in our samples.…”

Section: Discussionsupporting

confidence: 84%

“…This makes difficult to ascertain the real etiologic agent producing the disease and is an important handicap in the field studies. V. alginolyticus constitutes an opportunistic invader of already damaged tissues, or a weak pathogen of stressed fish, and has been isolated from water and/or sea bass tissues (Damir et al, 2013;Sadok et al, 2013) as in our study. A. hydrophila also produces skin ulcers and hemorrhagic septicemia but is rare in marine waters (Austin and Austin, 2007).…”

Section: Discussionmentioning

confidence: 99%

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Bacteriological, Metabolic and Immunological Evaluation of European sea Bass Reared in Ponds with Heated Water under a Natural Vibriosis-Like Outbreak

Mokrani¹

2019

PVJ

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Fish culture in ponds with heated water offers better fish growth compared to farms in natural sea water temperature, but the increase in temperature favors the development of bacteria and fish disease. In this study, in European sea bass (Dicentrarchus labrax) specimens reared in ponds with heated water from a power plant showing symptoms of a vibriosis-like disease we aimed to characterize the bacterial community as well as some serum metabolic and immune parameters. Bacteriological analysis revealed an important contamination with fecal coliforms, Pseudomonas aeruginosa, Vibrio alginolyticus, yeasts and molds whilst fish tissues from both healthy and diseased specimens were positive for total aerobic flora and several potential pathogenic bacteria such as V. alginolyticus, Aeromonas hydrophila, Pasteurella multocida, P. aeruginosa, Proteus vulgaris and P. mirabilis. Additionally, Grimontia hollisae was only present in diseased fish tissues. Serum metabolic parameters such as aspartate aminotransferase, bile acids, creatine kinase, uric acid, glucose, globulin and potassium were generally increased in diseased fish though only the levels of phosphorous reached a significant level compared to the healthy specimens. Regarding fish innate immunity, our results showed no statistical differences between healthy and diseased fish on peroxidase, protease or antiprotease activity while the bactericidal activity against V. anguillarum was significantly decreased in diseased fish. Lastly, the gene expression of il1b, c3, dic and mpx was down-regulated in the blood of diseased fish while ighm was up-regulated. To Cite ThisArticle: Mokrani D, Cerezuela R, Oumouna M, Esteban MÁ and Cuesta A, 2019. Bacteriological, metabolic and immunological evaluation of European sea bass reared in ponds with heated water under a natural vibriosis-like outbreak. Pak Vet J, 39(3): 329-334. http://dx.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Bacteriological, Metabolic and Immunological Evaluation of European sea Bass Reared in Ponds with Heated Water under a Natural Vibriosis-Like Outbreak

Mokrani¹

2019

PVJ

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“…play a significant role in the degradation of organic matter (Damir et al. ) and, hence, regulate the dissolved organic carbon to higher trophic levels of the marine food web (Grossart et al. ; Al‐saari et al.…”

Section: The Family Vibrionaceae: Causative Agents Of Vibriosismentioning

confidence: 99%

Vibriosis in Fish: A Review on Disease Development and Prevention

et al. 2018

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Current growth in aquaculture production is parallel with the increasing number of disease outbreaks, which negatively affect the production, profitability, and sustainability of the global aquaculture industry. Vibriosis is among the most common diseases leading to massive mortality of cultured shrimp, fish, and shellfish in Asia. High incidence of vibriosis can occur in hatchery and grow-out facilities, but juveniles are more susceptible to the disease. Various factors, particularly the source of fish, environmental factors (including water quality and farm management), and the virulence factors of Vibrio, influence the occurrence of the disease . Affected fish show weariness, with necrosis of skin and appendages, leading to body malformation, slow growth, internal organ liquefaction, blindness, muscle opacity, and mortality. A combination of control measures, particularly a disease-free source of fish, biosecurity of the farm, improved water quality, and other preventive measures (e.g., vaccination) might be able to control the infection. Although some control measures are expensive and less practical, vaccination is effective, relatively cheap, and easily implemented. In this review, the latest knowledge on the pathogenesis and control of vibriosis, including vaccination, is discussed. 4INA-SALWANY ET AL.

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“…Vibrio alginolyticus is one of the most common pathogenic marine Vibrio species and has been found to not only cause serious vibriosis in marine aquatic animals (Damir, Irena, Damir, & Emin, ; Gómezleón, Villamil, Lemos, Novoa, & Figueras, ; Kahlanakbi, Chaieb, & Bakhrouf, ; Sadok, Mejdi, Nourhen, & Amina, ), but also induce seafood‐poisoning or fatal extra‐intestinal infections in humans after consumption of raw or undercooked sea products (Lin, Ou, Dong, & Chen, ; Qiang, Qing, & Shen, ). Currently, antibiotics were mainly used in marine aquaculture to protect fish from V. alginolyticus infection.…”

Section: Introductionmentioning

confidence: 99%

Identification of fish source Vibrio alginolyticus and evaluation of its bacterial ghosts vaccine immune effects

Cao

Zhang

et al. 2018

MicrobiologyOpen

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Vibrio alginolyticus (V. alginolyticus) is a common pathogen for humans and marine aquatic animals. Vibriosis of marine aquatic animals, caused by V. alginolyticus, has become more prevalent globally in recent years. Hence, a safe and effective vaccine is urgently needed for the control of this disease. Here, the strain 16‐3 isolated from the large yellow croaker (Larimichthys crocea) suffered from canker was identified as V. alginolyticus based on morphological, biochemical, and 16S rDNA sequencing analysis. Then, recombinant temperature‐controlled lysis plasmid pBV220‐lysisE was electroporated into the strain 16‐3 to generate V. alginolyticus bacterial ghosts (VaBGs) by inducing lysis gene E expression, and the safety and immune effects of VaBGs were further investigated in mice and large yellow croaker. The results showed that VaBGs were as safe as formalin‐killed V. alginolyticus cells (FKC) to mice and fish. Compared with FKC and PBS groups, significant elevations of the serum agglutinating antibody titer, serum bactericidal activity, lymphocyte proliferative responses, and levels of four different cytokines (Th1 type: IL‐2, TNF‐α; Th2 type: IL‐4 and IL‐6) in serum were detected in the VaBGs group, indicating that a Th1/Th2‐mediated mixed immune response was elicited by the VaBGs. More importantly, after challenged with the parent strain 16‐3, VaBGs‐vaccinated mice and fish showed higher protection than FKC‐vaccinated mice, the relative percent of survival (RPS) being 60%, 66.7% and 40%, respectively. Taken together, this is the first demonstration that the newly constructed V. alginolyticus ghosts may be developed as a safe and effective vaccine against V. alginolyticus infection in aquaculture.

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Occurrence, characterization and antimicrobial susceptibility of Vibrio alginolyticus in the Eastern Adriatic Sea

Cited by 22 publications

References 24 publications

Bacteriological, Metabolic and Immunological Evaluation of European sea Bass Reared in Ponds with Heated Water under a Natural Vibriosis-Like Outbreak

Bacteriological, Metabolic and Immunological Evaluation of European sea Bass Reared in Ponds with Heated Water under a Natural Vibriosis-Like Outbreak

Vibriosis in Fish: A Review on Disease Development and Prevention

Identification of fish source Vibrio alginolyticus and evaluation of its bacterial ghosts vaccine immune effects

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