Occurrence, genotypes and antimicrobial susceptibility of Salmonella collected from the broiler production chain within an integrated poultry company

Vinueza-Burgos, Christian; Baquero, María Inés; Medina, José Luís; Zutter, Lieven De

doi:10.1016/j.ijfoodmicro.2019.03.014

Cited by 54 publications

(45 citation statements)

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“…This issue is especially evident in S . Infantis, as already been seen in Ecuador ( 7 , 8 ) and other countries of the region ( 63 – 65 ). Although in a lower extent, this feature has also been reported in Europe where high rates of MDR (up to 85%) are reported in S .…”

Section: Discussionmentioning

confidence: 52%

“…The prevalence of Salmonella was high in poultry farms and chicken carcasses, similarly to other studies in the region ( 6 – 8 , 51 ). Although this research did not look for Salmonella in earlier stages of the broiler production chain, previous studies in Ecuador have reported the importance of compound feed, 1-day-old chicks and broiler pens in the Salmonella exposure of broilers ( 7 , 52 ). These results highlight the necessity to improve broilers production systems in Ecuador toward a better control of Salmonella in the food chain.…”

Section: Discussionmentioning

confidence: 95%

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Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

et al. 2020

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Salmonella enterica is one of the most important foodborne pathogens around the world. In the last years, S . enterica serovar Infantis has become an important emerging pathogen in many countries, often as multidrug resistant clones. To understand the importance of S. enterica in the broiler industry in Ecuador, we performed a study based on phenotypic and WGS data of isolates from poultry farms, chicken carcasses and humans. We showed a high prevalence of S. enterica in poultry farms (41.4%) and chicken carcasses (55.5%), but a low prevalence (1.98%) in human samples. S . Infantis was shown to be the most prevalent serovar with a 98.2, 97.8, and 50% in farms, foods, and humans, respectively, presenting multidrug resistant patterns. All sequenced S . Infantis isolates belonged to ST32. For the first time, a pESI-related megaplasmid was identified in Ecuadorian samples. This plasmid contains genes of antimicrobial resistance, virulence factors, and environmental stress tolerance. Genomic analysis showed a low divergence of S . Infantis strains in the three analyzed components. The results from this study provide important information about genetic elements that may help understand the molecular epidemiology of S . Infantis in Ecuador.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 95%

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

et al. 2020

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“…The emergence of the S. Infantis serotype has been described in European countries and the United States [ 4 , 41 , 42 , 43 , 44 ]. In South America, it has also been described in Ecuador [ 45 ] and Peru [ 46 ]. In Brazil, S. Infantis has been catalogued as the second most prevalent serotype in broilers [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in Salmonella Infantis Strains Isolated from Chicken Meat: First Findings in Chile

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Cornejo

Zavala

et al. 2020

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Salmonella Infantis is a zoonotic pathogen that causes gastroenteritis in humans and animals, with poultry being its main reservoir. In Chile, there are no data to characterize S. Infantis strains in poultry production. In this study, 87 S. Infantis strains were isolated from chicken meat for sale in supermarkets in Santiago, Chile, and characterized according to their virulence genes, biofilm formation abilities, antibiotic susceptibility, and resistance genes. Through polymerase chain reaction or PCR, the strains were analyzed to detect the presence of 11 virulence genes, 12 antibiotic resistance genes, and integrase genes. Moreover, disc diffusion susceptibility to 18 antimicrobials and the ability to form biofilm in vitro were evaluated. Results demonstrated six different virulence gene profiles. Ninety-four percent of the strains were multi-resistant to antibiotics with weak biofilm formation abilities, 63.2% of the strains were broad spectrum β- lactam resistant, and the bla CTX-M-65 gene was amplified in 13 strains. Only 3.4% of the strains were fluoroquinolone resistant, and the qnrB gene was amplified in two strains. Colistin resistance was exhibited in 28.7% of the strains, but mrc genes were not amplified in any strain under study. The isolated S. Infantis strains are pathogenic and antibiotic multi-resistant, and thus, this Salmonella serotype should be under surveillance in the poultry food production chain with the aim of protecting public health.

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“…Salmonella is a common pathogen that can survive and pass through the technological steps of poultry production ( Vinueza-Burgos et al., 2019 ). Human gastrointestinal infections caused by Salmonella usually are associated with the consumption of poultry products; therefore, the control of this type of pathogens is of great importance ( Wegener et al., 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Study of the antibiotic residues in poultry meat in some of the EU countries and selection of the best compositions of lactic acid bacteria and essential oils against Salmonella enterica

et al. 2020

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In this study, the presence of antibiotics ( ANB ) residues was evaluated in poultry meat purchased from German and Lithuanian markets. In addition, the antimicrobial activity of 13 lactic acid bacteria ( LAB ) strains, 2 essential oils ( EO ) ( Thymus vulgaris and Origanum vulgare L.), and their compositions were tested for the purpose of inhibiting antibiotic-resistant Salmonella spp. ANB residues were found in 3 out of the 20 analyzed poultry meat samples: sample no. 8 contained enrofloxacin (0.46 μg/kg), sample no. 14 contained both enrofloxacin and doxycycline (0.05 and 16.8 μg/kg, respectively), and sample no. 18 contained enrofloxacin (2.06 μg/kg). The maximum residue limits ( MRLs ) for the sum of enrofloxacin and ciprofloxacin and for doxycycline in the poultry muscle are 100 μg/kg. Finally, none of the tested poultry meat samples exceeded the suggested MRLs; however, the issue of ANB residues still requires monitoring of the poultry industry in Germany, Poland, and Lithuania, despite the currently established low ANB concentrations. These findings can be explained by the increased use of alternatives to ANB in the poultry industry. Our results showed that an effective alternative to ANB, which can help to reduce the occurrence of antibiotic-resistant salmonella, is a composition containing 1.0% of thyme EO and the following LAB strains: Lactobacillus plantrum LUHS122, Enteroccocus pseudoavium LUHS242, Lactobacillus casei LUHS210, Lactobacillus paracasei LUHS244, Lactobacillus plantarum LUHS135, Lactobacillus coryniformins LUHS71, and Lactobacillus uvarum LUHS245, which can be recommended for poultry industry as components of feed or for the treatment of surfaces, to control the contamination with Salmonella strains. However, it should be mentioned that most of the tested LAB strains were inhibited by thyme EO at the concentrations of 0.5 and 1.0%, except for LUHS122, LUHS210, and LUHS245. Finally, it can be noted that the agents responsible for the inhibitory effect on Salmonella are not the viable LAB strains but rather their metabolites, and further studies are needed to identify which metabolites are the most important.

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Occurrence, genotypes and antimicrobial susceptibility of Salmonella collected from the broiler production chain within an integrated poultry company

Cited by 54 publications

References 48 publications

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in Salmonella Infantis Strains Isolated from Chicken Meat: First Findings in Chile

Study of the antibiotic residues in poultry meat in some of the EU countries and selection of the best compositions of lactic acid bacteria and essential oils against Salmonella enterica

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