Occurrence, molecular characterization and predominant genotypes of Enterocytozoon bieneusi in dairy cattle in Henan and Ningxia, China

Li, Junqiang; Luo, Nannan; Wang, Chenrong; Qi, Meng; Cao, Jianke; Cui, Zhaohui; Huang, Jianying; Wang, Rongjun; Zhang, Longxian

doi:10.1186/s13071-016-1425-5

Cited by 66 publications

(53 citation statements)

References 23 publications

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“…The prevalence of this parasite in cattle was 11.9% (33/277), in accordance with a previous study in Henan (Li et al. ). This infection rate, however, is higher than some studies previously conducted in Henan (6.4%, 19/298) and northeastern China (6.0%, 32/537), and lower than a large survey of E. bieneusi in cattle in Hebei (19.4%, 202/1040) (Hu et al.…”

Section: Discussioncontrasting

confidence: 56%

“…Microsporidia were previously classified as protozoa, but have been recently reclassified as fungi (Didier and Weiss ; Li et al. ; Matos et al. ).…”

mentioning

confidence: 99%

“…Over 240 E. bieneusi genotypes have been identified in humans and animals, based on DNA sequence analysis of the ribosomal internal transcribed spacer (ITS) (Li et al. ; Santín and Fayer ; Thellier and Breton ; Zhao et al. ).…”

mentioning

confidence: 99%

“…MICROSPORIDIOSIS is an emerging, opportunistic disease in humans and other animals, caused by microsporidia, a complex group of obligate intracellular eukaryotes. Microsporidia were previously classified as protozoa, but have been recently reclassified as fungi (Didier and Weiss 2006;Li et al 2016;Matos et al 2012). To date, among approximately 1,300 microsporidian species in 160 genera formally described, Enterocytozoon bieneusi is the most frequently detected species in humans Keeling 2009).…”

mentioning

confidence: 99%

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The Potential Role of Synanthropic Rodents and Flies in the Transmission of Enterocytozoon bieneusi on a Dairy Cattle farm in China

Zhao

et al. 2018

J Eukaryotic Microbiology

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Enterocytozoon bieneusi causes microsporidiosis, a condition with complex epidemiology involving both direct and indirect transmission routes. To assess the potential role of synanthropic rodents and flies in the transmission of this pathogen, a total of 277 cattle fecal samples, 199 synanthropic rodents, and 50 batches of 20 flies were collected from a cattle farm. These samples were screened for the presence of E. bieneusi by PCR and sequencing of the internal transcribed spacer (ITS) region of the rRNA gene. The positive rates of cattle, synanthropic rodents, and flies were 11.9% (33/277), 4.0% (8/199) and 12.0% (6/50), respectively. Nineteen genotypes were identified, including 11 known genotypes (BEB6, I, COS-I, EbpC, D, J, CHS5, CHG1 to CHG3 and CHG14) and eight novel genotypes (named CHC9 to CHC16). The dominant genotype detected in the present study, BEB6, was found in all three categories of hosts. Moreover, human pathogenic genotypes D and EbpC were also observed in both synanthropic rodents and flies. These results demonstrate that synanthropic rodents and flies may act as biological disseminator or mechanical vector in the transmission of microsporidiosis to humans. Efforts should be made to minimize threats from these commensal animals to public health.

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Section: Discussioncontrasting

confidence: 56%

“…Microsporidia were previously classified as protozoa, but have been recently reclassified as fungi (Didier and Weiss ; Li et al. ; Matos et al. ).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The Potential Role of Synanthropic Rodents and Flies in the Transmission of Enterocytozoon bieneusi on a Dairy Cattle farm in China

Zhao

et al. 2018

J Eukaryotic Microbiology

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“…), CHC5 isolates have been found in dairy cattle and wild boar (Li et al. , ). This study shows the capacity of CHG19 and CHC5 isolates to adapt to different pig breeds.…”

Section: Discussionmentioning

confidence: 99%

Multilocus Typing of Enterocytozoon bieneusi in Pig Reveals the High Prevalence, Zoonotic Potential, Host Adaptation and Geographical Segregation in China

Zheng

Zhou

et al. 2019

J Eukaryotic Microbiology

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Enterocytozoon bieneusi is one of the most frequently diagnosed Microsporidia of humans and most animals. However, there is no information on E. bieneusi infection of pigs in Tibet and Henan, China. In this study, 1,190 fecal samples were collected from pigs in Tibet and Henan and screened for the presence of E. bieneusi. The overall prevalence of E. bieneusi infection was 54.2% (645/1,190), with differences in prevalence observed among geographical areas, ages, and pig breeds. Moreover, 10 E. bieneusi genotypes were identified based on internal transcribed spacer region genotyping, including eight known genotypes (EbpC, EbpA, CHG19, CHC5, Henan‐III, I, D, and H) and two novel genotypes (XZP‐I and XZP‐II). Multilocus sequence typing revealed 18, 7, 17, and 13 genotypes at minisatellite/microsatellite loci MS1, MS3, MS4, and MS7, respectively. Strong linkage disequilibrium (LD) and few numbers of recombination events, suggest a clonal structure of the E. bieneusi population examined in this study. The low pairwise genetic distance (FST) and gene flow (Nm) values indicated limited gene flow in the E. bieneusi population from different hosts, with phylogenetic, structure, and median‐joining network analyses all indicating the existence of host and geographical isolation. The identification of isolates belonging to nine human‐pathogenic genotypes indicates that pigs play an important role in the dissemination of E. bieneusi, improving our present understanding of E. bieneusi epidemiology in the studied region.

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Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks

Zhang

Cai

et al. 2018

Parasitol Res

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Few studies have been conducted on the distribution of Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks, which live outdoors in extreme climate with high altitude. In this study, fecal specimens from 312 Tibetan sheep and 554 yaks in Qinghai, China, were collected and examined for E. bieneusi by PCR-sequence analysis of the ribosomal internal transcribed spacer. Among them, 73 (23.4%) specimens from Tibetan sheep and 40 (7.2%) from yaks were positive for E. bieneusi. There were eight E. bieneusi genotypes in Tibetan sheep, including three known ones (BEB6, COS-I, and NESH5) and five novel ones (named as CHS13-CHS17). Similarly, seven E. bieneusi genotypes were found in yaks, including five known ones (J, BEB4, BEB6, COS-I, and NESH5) and two novel ones (named as CHN13 and CHN14). Most of the E. bieneusi genotypes and all frequent ones identified in the study belonged to group 2. One new subgroup of genotypes was identified within group 1. The distribution of E. bieneusi genotypes was different between Tibetan sheep and yaks, with BEB6 as the dominant one (42.5%) in Tibetan sheep and J as the dominant one (47.5%) in yaks. These data support the occurrence of host adaptation among E. bieneusi genotypes within group 2.

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Occurrence, molecular characterization and predominant genotypes of Enterocytozoon bieneusi in dairy cattle in Henan and Ningxia, China

Cited by 66 publications

References 23 publications

The Potential Role of Synanthropic Rodents and Flies in the Transmission of Enterocytozoon bieneusi on a Dairy Cattle farm in China

The Potential Role of Synanthropic Rodents and Flies in the Transmission of Enterocytozoon bieneusi on a Dairy Cattle farm in China

Multilocus Typing of Enterocytozoon bieneusi in Pig Reveals the High Prevalence, Zoonotic Potential, Host Adaptation and Geographical Segregation in China

Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks

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