Occurrence of a Diploid Type and a New First Intermediate Host of a Human Lung Fluke, Paragonimus westermani, in Korea

Park, Gab Man; Lee, K.J.; Im, Kyung Il; Park, H.; Yong, Tai-Soon

doi:10.1006/expr.2001.4668

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…Metacercariae of Paragonimus westermani were recovered from naturally infected crayfish collected from Youngam, for triploid P. westermani, an indigenous area, Jeollanam-do, Korea (Park GM et al, 2001). Collected crayfish were digested with artificial digestive juice by incubation for 2 h at 37°C.…”

Section: Collection Of Parasitesmentioning

confidence: 99%

Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani

Choi

Lee

et al. 2006

Korean J Parasitol

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Abstract:The mammalian trematode Paragonimus westermani is a typical digenetic parasite, which can cause paragonimiasis in humans. Host tissues and blood cells are important sources of nutrients for development, growth and reproduction of P. westermani. In this study, a cDNA clone encoding a 47 kDa hemoglobinase of P. westermani was characterized by sequencing analysis, and its localization was investigated immunohistochemically. The phylogenetic tree prepared based on the hemoglobinase gene showed high homology with hemoglobinases of Fasciola hepatica and Schistosoma spp. Moreover, recombinant P. westermani hemoglobinase degradaded human hemoglobin at acidic pH (from 3.0 to 5.5) and its activity was almost completely inhibited by E-64, a cysteine proteinase inhibitor. Immunohistochemical studies showed that P. westermani hemoglobinase was localized in the epithelium of the adult worm intestine implying that the protein has a specific function. These observations suggest that hemoglobinase may act as a digestive enzyme for acquisition of nutrients from host hemoglobin. Further investigations may provide insights into hemoglobin catabolism in P. westermani.

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Section: Collection Of Parasitesmentioning

confidence: 99%

Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani

Choi

Lee

et al. 2006

Korean J Parasitol

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“…1989; Park et al . 2001). Paragonimus westermani metacercariae from Sri Lanka (656 × 465 µ m) and India (420·6–1012·7 µ m) were more oval and much larger in size with a thin inner cyst wall (Iwagami et al .…”

Section: Discussionmentioning

confidence: 99%

Molecular and morphological variation of Paragonimus westermani in Vietnam with records of new second intermediate crab hosts and a new locality in a northern province

Doanh

Bui

et al. 2016

Parasitology

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Paragonimus westermani is one of the most medically important lung flukes and is widely distributed in Asia. It exhibits considerable variation in morphological, genetic and biological features. In central provinces of Vietnam, a high prevalence of metacercariae of this species has been reported from the crab intermediate host, Vietopotamon aluoiense. In this study, we detected P. westermani metacercariae in two additional crab hosts, Donopotamon haii in Quang Tri Province, central Vietnam and Indochinamon tannanti in Yen Bai Province in the north. The latter is a new locality for P. westermani in a northern region of Vietnam where P. heterotremus is the only species currently known to cause human paragonimiasis. Paragonimus westermani metacercariae found in Vietnam showed considerable morphological variation but slight genetic variation based on DNA sequences from the nuclear ribosomal ITS2 region and the mitochondrial 16S gene. Co-infection of the same individual crabs with P. westermani and P. heterotremus and/or some other Paragonimus species was found frequently, suggesting potential for co-infection in humans. The findings of the present study emphasize the need for highly specific molecular and immunodiagnostic methods to differentially diagnose between P. westermani and P. heterotremus infections.

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“…Experimental dogs were orally challenged with the metacercariae of P. westermani , which were collected from naturally infected crayfish in endemic areas of Korea (Haenam, 2n; Bogil-do and Youngam, 3n) and China (Nanchang, Jiangxi Province, 2n) [ 39 ]. Five months after the infections, adult worms of the parasite were harvested from the dogs' lungs.…”

Section: Methodsmentioning

confidence: 99%

PwRn1, a novel Ty3/gypsy-like retrotransposon of Paragonimus westermani: molecular characters and its differentially preserved mobile potential according to host chromosomal polyploidy

et al. 2008

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Occurrence of a Diploid Type and a New First Intermediate Host of a Human Lung Fluke, Paragonimus westermani, in Korea

Cited by 10 publications

References 15 publications

Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani

Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani

Molecular and morphological variation of Paragonimus westermani in Vietnam with records of new second intermediate crab hosts and a new locality in a northern province

PwRn1, a novel Ty3/gypsy-like retrotransposon of Paragonimus westermani: molecular characters and its differentially preserved mobile potential according to host chromosomal polyploidy

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