Occurrence of genes coding for MSCRAMM and biofilm-associated protein Bap in Staphylococcus spp. isolated from bovine subclinical mastitis and relationship with somatic cell counts

Zuniga, Eveline; Melville, Priscilla Anne; Saidenberg, André Becker Simões; Laes, Marco Antonio; Gonsales, Fernanda Fidelis; Salaberry, Sandra Renata Sampaio; Gregori, Fábio; Brandão, Paulo Eduardo; Santos, Franklin Gerônimo Bispo; Lincopán, Nilton; Benites, N. R.

doi:10.1016/j.micpath.2015.08.014

Cited by 31 publications

(33 citation statements)

References 17 publications

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“…(189 samples) isolated from bovine subclinical mastitis. Apart from this, frequency of bap gene occurrence was significantly higher in coagulase-negative strains as compared with coagulasepositive 21 . The involvement of polysaccharide-intercellular adhesin (ica-dependent) component of the S. aureus biofilm matrix has been studied comprehensively 22 .…”

Section: Introductionmentioning

confidence: 78%

Staphylococcus aureusBiofilm Removal by Targeting Biofilm-Associated Extracellular Proteins

Shukla

Rao

2017

Preprint

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Abstract:Aim: Among cell surface proteins, biofilm-associated protein promotes biofilm development in Staphylococcus aureus strains. Aim of this study was to investigate proteinase-mediated biofilm dispersion in different isolates of S. aureus. Methods and Results:Microtitre plate based biofilm assay showed that 2 µg/mL proteinase K significantly inhibited biofilm development in bap-positive S. aureus V329 as well as other S. aureus strains, i.e. SA7, SA10, SA33, SA352 and but not in bap-mutant M556 and SA392 (a weak biofilm producing strain). However, proteinase K treatment on S. aureus planktonic cells showed that there was no inhibition of planktonic growth at any concentration of proteinase K when tested up to 32 µg/mL. This observation ruled out the possibility of S. aureus biofilm inhibition by altering the cell viability. Proteinase K treatment upon 24 h old preformed biofilms showed an enhanced dispersion of bap-positive V329 and SA7, SA10, SA33 and SA352 biofilms, however, proteinase K did not affect the bap-mutant S. aureus M556 and SA392 biofilms. Biofilm compositions study before and after proteinase K treatment indicated that Bap might also be involved in eDNA retention in the biofilm matrix that aid in biofilm stability. When proteinase K was used in combination with antibiotics, a synergistic effect in antibiotic efficacy was observed against all biofilm forming S. aureus strains.. CC-BY-NC4.0 International license not peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was . http://dx.doi.org/10.1101/102384 doi: bioRxiv preprint first posted online Jan. 23, 2017; Conclusion:Proteinase K inhibited biofilms growth in S. aureus bovine mastitis isolates but did not affect their planktonic growth. An enhanced dispersion of preformed S. aureus biofilms was observed upon proteinase K treatment. Proteinase K treatment with antibiotics showed a synergistic effect against S. aureus biofilms.Significance of the study: The study suggests that dispersing S. aureus by protease can be of use while devising strategies against S. aureus biofilms. Proteinase K treatment has a wider scope for control of S. aureus biofilms.

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Section: Introductionmentioning

confidence: 78%

Staphylococcus aureusBiofilm Removal by Targeting Biofilm-Associated Extracellular Proteins

Shukla

Rao

2017

Preprint

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“…The bap gene has been identified in up to 25% S. aureus isolates and up to 95% of CNS isolates from bovine mastitis [144,145], and up-regulation of the bap gene with the presence of low concentrations of milk or lactose in the growth medium has been shown in vitro [141,146]. Zuniga et al studied the presence of genes encoding Bap and a group of adhesins in staphylococci isolated from subclinical mastitis [147]. The median somatic cell counts, which are markers of sub-clinical mastitis, were higher in milk samples where the bacteria had the bap gen and the adhesin genes eno, fnbA, fib, than in samples with staphylococci without these genes.…”

Section: Mastitismentioning

confidence: 99%

Bacterial Biofilm and its Role in the Pathogenesis of Disease

et al. 2020

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Recognition of the fact that bacterial biofilm may play a role in the pathogenesis of disease has led to an increased focus on identifying diseases that may be biofilm-related. Biofilm infections are typically chronic in nature, as biofilm-residing bacteria can be resilient to both the immune system, antibiotics, and other treatments. This is a comprehensive review describing biofilm diseases in the auditory, the cardiovascular, the digestive, the integumentary, the reproductive, the respiratory, and the urinary system. In most cases reviewed, the biofilms were identified through various imaging technics, in addition to other study approaches. The current knowledge on how biofilm may contribute to the pathogenesis of disease indicates a number of different mechanisms. This spans from biofilm being a mere reservoir of pathogenic bacteria, to playing a more active role, e.g., by contributing to inflammation. Observations also indicate that biofilm does not exclusively occur extracellularly, but may also be formed inside living cells. Furthermore, the presence of biofilm may contribute to development of cancer. In conclusion, this review shows that biofilm is part of many, probably most chronic infections. This is important knowledge for development of effective treatment strategies for such infections.

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“…Sung et al (2008); Vautor et al (2008), and Szweda et al (2012) did not find this gene among S. aureus isolated from bovine mastitis specimens, whereas Darwish and Asfour (2013) found only one isolate harbouring the bap gene. Cucarella et al (2004) in Spain and Zuniga et al (2015) in Brazil reported that 25.6 % and 15.8 % of S. aureus isolates from bovine mastitis samples harboured this gene, respectively. Vautor et al (2008) reported that the prevalence of the bap gene among S. aureus isolates, including bovine mastitis isolates, was very low.…”

Section: Biofilm Formationmentioning

confidence: 99%

Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms

et al. 2016

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Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.

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Occurrence of genes coding for MSCRAMM and biofilm-associated protein Bap in Staphylococcus spp. isolated from bovine subclinical mastitis and relationship with somatic cell counts

Cited by 31 publications

References 17 publications

Staphylococcus aureusBiofilm Removal by Targeting Biofilm-Associated Extracellular Proteins

Staphylococcus aureusBiofilm Removal by Targeting Biofilm-Associated Extracellular Proteins

Bacterial Biofilm and its Role in the Pathogenesis of Disease

Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms

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