Occurrence of insertion sequences within the genomes and Tn-like elements of glycopeptide-resistant enterococci isolated in Brazil, and identification of a novel element, IS

Camargo, Ilana Lopes Baratella da Cunha; Zanella, Rosemeire Cobo; Brandileone, Maria C. C.; Pignatari, Antônio Carlos Campos; Goldman, Gustavo H.; Woodford, Neil; Darini, Ana Lúcia da Costa

doi:10.1016/j.ijmm.2004.11.003

Cited by 23 publications

(19 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our clinical isolates were characterized by IS1216V insertion in the orf2-vanR region and IS1542 insertion in the vanX-vanY region (Table 1). These results are similar to the results of other researchers (Handwerger et al, 1995;MacKinnon et al, 1997;Willems et al, 1999;Yu et al, 2003;Huh et al, 2004;Camargo et al, 2005;Jung et al, 2006a;Park et al, 2007). Changes in the phenotype of VRE carrying the vanA gene have been reported in many countries (Jensen et al, 1999;Hashimoto et al, 2000;Lauderdale et al, 2002;Park et al, 2007Park et al, , 2011Gu et al, 2009).…”

Section: Molecular Epidemiology Of Korean Humans and Poultrysupporting

confidence: 90%

Comparison of genetic epidemiology of vancomycin-resistant Enterococcus faecium isolates from humans and poultry

Cha

Jung

Lee

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

2 (i)-ro, Osong-eup, Cheongwon-gun, Chungcheongbuk-do 363-951, Republic of Korea This study was conducted to investigate the molecular characteristics and genetic relatedness of vancomycin-resistant Enterococcus faecium (VREF) isolates obtained from humans and poultry in Korea. A total of 147 VREF isolates from humans (71 clinical isolates) and poultry (76 isolates) in Korea were compared with respect to their antibiotic susceptibilities, organization of the Tn1546 transposon element, detection of virulence genes (esp and hyl), pulsed-field gel electrophoresis (PFGE) typing and multilocus sequence typing (MLST). All of the human and poultry isolates had the vanA gene and 11.3 % (8/71) of the clinical isolates showed the VanB phenotype/vanA genotype. PCR mapping of the Tn1546 elements was different for isolates of the two groups: human isolates were classified into five transposon types, whereas all poultry isolates were identical to Tn1546 of E. faecium strain BM4147. The esp gene was detected in both human (93.0 %, 66/71) and poultry (26.3 %, 20/76) isolates, as was the hyl gene (human isolates: 80.3 %, 57/71; poultry isolates: 26.3 %, 20/76). Using MLST, the 71 human isolates could be divided into ten sequence types (STs) belonging to clonal complex (CC) 17 (except for one singleton). The 76 poultry isolates were categorized into 14 STs and 88.2 % (67/76) of the poultry isolates belonged to CC26. PFGE typing of the human isolates demonstrated diverse PFGE profiles among the strains. However, the PFGE patterns of the poultry isolates were possibly related to the strains collected from individual farms. These data suggest that epidemic clonal groups of human and poultry VREFs in Korea have evolved through different evolutionary processes.

show abstract

Section: Molecular Epidemiology Of Korean Humans and Poultrysupporting

confidence: 90%

Comparison of genetic epidemiology of vancomycin-resistant Enterococcus faecium isolates from humans and poultry

Cha

Jung

Lee

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Integration appeared 17 bp after the 3 0 end of the vanS gene. However, similar IS1251 insertions were found recently also in VREF from Brazil, Ireland and Norway (Camargo et al, 2004(Camargo et al, , 2005Simonsen et al, 2000). Whereas the Norwegian and our two clusters (D and D1) show an identical DNA sequence for IS1251, the US/Brazilian VREF showed only 98% identity with the former IS1251 sequence contradicting a recent intercontinental spread.…”

Section: Discussionsupporting

confidence: 78%

Increasing rates of vancomycin resistance among Enterococcus faecium isolated from German hospitals between 2004 and 2006 are due to wide clonal dissemination of vancomycin-resistant enterococci and horizontal spread of vanA clusters

Werner

Klare

Fleige

et al. 2008

International Journal of Medical Microbiology

View full text Add to dashboard Cite

“…Presence of a transposase (IS204/IS1001/IS1096/IS1165) between vanY and vanX was partially resolved. This vanA cluster constitutes a subtype of Tn1546-like elements which often bear IS elements like ISEf1, IS1678, or IS1216 V, ISEfa5, IS19-like between vanX and vanY (Werner et al, 2008;Jung et al, 2007;Camargo et al, 2005;Lopez et al, 2010). ermB, which encodes resistance to macrolides, lincosamides, and streptogramin B antibiotics was also identified on pLG1.…”

Section: Resistancementioning

confidence: 99%

A multiresistance megaplasmid pLG1 bearing a hylEfm genomic island in hospital Enterococcus faecium isolates

Gomez

Schaik

Freitas

et al. 2011

International Journal of Medical Microbiology

View full text Add to dashboard Cite

Occurrence of insertion sequences within the genomes and Tn-like elements of glycopeptide-resistant enterococci isolated in Brazil, and identification of a novel element, IS

Cited by 23 publications

References 29 publications

Comparison of genetic epidemiology of vancomycin-resistant Enterococcus faecium isolates from humans and poultry

Comparison of genetic epidemiology of vancomycin-resistant Enterococcus faecium isolates from humans and poultry

Increasing rates of vancomycin resistance among Enterococcus faecium isolated from German hospitals between 2004 and 2006 are due to wide clonal dissemination of vancomycin-resistant enterococci and horizontal spread of vanA clusters

A multiresistance megaplasmid pLG1 bearing a hylEfm genomic island in hospital Enterococcus faecium isolates

Contact Info

Product

Resources

About